Other Sciences

Hi, I did electroporation to insert to BL21 DE3 cells with pACDK4C plasmid which contains Cap resistance gene and Targetron (intron that can integrate into a specific location) that inserts to LacZ gene. The Taregetron cannot be transcribe without IPTG presence due to the fact that it located downstream to T7 promoter and Lac operator. I plated the transformants on LB+Cap (25ug/ml) plate. As negative control I plated BL21 cells without the plasmid on LB plate. Both plates were applied with 60ul Xgal (20ug/ml) + 40ul IPTG 0.1M. The strange thing was that in the LB+Cap plates the cells only grow in the edges of the plate while in the negative control the colonies…

http://www.newindpress.com/NewsItems.asp?ID=IE320050424061423&Page=3&Title=Features+-+Health+%26+Science&Topic=166 http://www.sciencedaily.com/releases/2005/04/050422165917.htm Also known as "microbial fuel cells", this technology is interesting because it suggests less energy-expensive ways to produce hydrogen, one of the biggest barriers in the search for alternative fuels.

I was looking at this very bad picture from another thread on this Forum. 1 Anyone should know some basic principle of perspective: when a photographer takes a picture horizontally from eye level, the eyes of a person standing upright are approximativaly at horizon level, as shown in the following picture. The rule does not count for the guy in the background: it is a strong indication that it is bad photoshop. 2 If the ground is horizontal all around, all other person standing upright must have his eyes at horizon level, no matter the distance from the photographer. That is because the horizon itself is a line at eye level, by definition. So, if the ground i…

For a long time, it's said bluetooth could cause cancer which is something I don't believe because if that's the case, it should have risen concern by the general public. However, I hope this is not a stupid question. What if a bluetooth device is made very poorly for sake of reducing the cost, or if it is made by an unqualified someone or factory, would it increase the any health risk to the end user? Or that said, is level or amount of radiation emitted relate to the how well the bluetooth is made?

Hi all, I was hoping someone could possibly shed some light on something that has been baffling me over the past few days... My girlfriend purchased a box of Lemonade Icy Poles the other day and put them in the freezer, all frozen as per normal. A few days later she came over and decided to bring two of them with her, the trip is approximately 15 minutes in the middle of summer and despite good air conditioning in her car they had melted, not completely though. Once she arrived they were immediately put in the freezer. This is where I get confused.. the next day we remembered the Icy Poles and decided to have them, however when I opened the freezer they were …

I have heard about ball lightning many times. Do any of you know what it is or what causes it? Or, have you seen it? http://www.amasci.com/weird/unusual/bl.html

Did anyone watch the national geographic channel special on lightning? What did you guys think of the ball lightning part? Was it all hype or could there be some new physics there?

This is acommunity idea as a branch off from this thread. The idea is to make a list of between 10 and 15 basic science questions any HS graduate should know the answer to. If you have any ideas of what should be included please post them here... at the end we can then compare our final results with those presented in the link given by the other thread Cheers, Ryan Jones

For my work, I utilise AWS Batch Service. Instead of sending the logs produced by AWS Batch to Cloud-Watch, I want to transmit them directly to Splunk. How can I set up the AWS Batch log-driver to accomplish this?

Bats have a clever systems built into them to help them see in the dark using sound I'm assuming most of you know roughly how it works http://science.howstuffworks.com/bat2.htm http://en.wikipedia.org/wiki/Bat However seeing in sound instead of light means they see in a completely different way, for example among other things the objects density and surface qualities of the objects like is it rough or furry or smooth or at an angle would have a large effect on how sound is deflected by the object in a very different way to light, also sources of sound are difrent than those of light. loud sound seems travels though walls for example to annoy the nabours when…

I was fooling around with a 9V battery recently, sticking graphite across the top, but the label said it could explode. How is that? Oh, and one of my teachers said if you ingested the battery acid it would kill you and there's nothing they can do about it. Is that true (I doubt it)?

Changing batteries in a friend's remote I noticed this as in the picture. I have never seen a battery with the positive terminal at the black end and the negative at the red end before. Comments ?

Why do batteries have a shelf life, for, say, 6 years? Why can't you let them sit there indefinitely and then use them? Please, don't make it too complicated for me to understand.

I discussed the idea of a brain computer interface with my 17 year old sister. At first, she seemed enamoured of the idea and could see many benefits. With further thought, she then became squeamish about the surgery that would be necessary for a true nervous system interface to function, and about the possibilities of a networked device attached to her body being hacked or otherwise having security liabilities. Then she decided that after all, it would be a very bad idea to have this kind of constant, easy access to something like the internet, or even the contents of her own hard drive, such as games and so on. She said that she herself had wasted many long hou…

http://saturn.jpl.nasa.gov/multimedia/videos/movies/pia08118-320-cc.mov has everything sky mountains rivers lakes (dry though) water ice rocks and hydrocarbon/ice rocks drainage channels, the landing site the whole experience of floating down by parachute clear day on Titan, no methane rain, otherwise I think you get a pretty complete show

1:00 am. Tired. Too much MatLab. Too much genetics. Need a good laugh. Solution; a free visit to M. Behe's website, Mr. Irreductible complexity. Very funny. First of all, unlike most scientists, Behe doesn't feel the need to use visually appealing graphics. Nooooooooooooooooo. But, he got a mousetrap. I could not believe this, I mean, this guy must sit on more $$$$ than most evolutionary theorists (of course, the source is different). Right after that, there's the "the Lehigh university is ashamed of my ideas but for some reason hasn't fired me yet" disclaimer. Which is also quite unusual. But hey, when you're a révolutionaire... And at…

what is it? i tried wikipedia, but couldn't understand the article.

So, I've been studying and i would like some opinions on the Bermuda Triangle. Thus far i have not found any geographical or climatological basis on which to lay the oddly large nuber of strange phenomena that claim ships and planes and such in the triangle. Im really interested in opinions. what do ya'll think?

Can anyone guide about this place,its location.And what type of research are the scientists doing on it?

What are in your case, the best or most mysterious cryptid out there? Tell the story of of it and what it might have been. THE BLOOP The Bloop was a sound that was heard in 1997 and was so loud that the "creature" that created it would have been the size of a mountain . It is consistent with icequakes generated by icebergs colliding or scraping each other. However, it sounded like it had a biological origin, and who knows. Maybe there is an Everest sized whale out there. According to Wikipedia, bloop is the name given to an ultra-low-frequency and extremely powerful underwater sound detected by the U.S. National Oceanic and Atmospheric Administration (NOAA) in 1997. Th…

I just got a new gym set and I'm going to start on a workout routine. I haven't figured out what I want to do, but I think I'm going to do some weight training. I also want to lose a bit of fat. Is it possible to build muscle and lose fat at the same time? I mean, they say that to build muscle, you have to eat a lot (a lot of protein and a lot of carbs), but to lose weight, you need to consume less than you're burning. Is it possible to do both at the same time?

I have an optical mouse, not a ball mouse. My dad told me that optical mouses work best on white surfaces, but from usage I find that red surfaces work best. Does it matter what is the color of the surface on which you use your optical mouse?

How exactly does this work? I've think that nitrogen is pumped into a sample and the amount that is pumped in can be used to measure it's surface area. Am I right?

Velociraptors had eyes at the sides of their heads right? If that's the case, and since it had binocular vision, could this dinosaur see better or worse than humans?

Do we need better intellect here to discuss science or should it be open for everyone to discuss? Atleast good intelligence is required.