Biochemistry and Molecular Biology

I wish to discuss a Biochemistry-Biophysics based treatment of Cancer, that has been languishing for more than 25 years because it is not patentable. A 1985 study published in the journal Cancer Research showed 98% reduction of tumors in 5 hours over 5 days of treatment? Strangely there were no follow ups. Thanks. Jay

How do humans produce it and what are the nutritional cofactors? Is it also produced in the brain? Your help is appreciated.

Hi all, Looking for a bit of help finding articles to show how Calories related to bodily processes. I know a Calorie (kcal) is the amount of energy needed to raise the temperature of 1 kilogram of water 1 degree Celsius. I understand the Atwater system uses the average values of 4 Kcal/g for protein, 4 Kcal/g for carbohydrate, and 9 Kcal/g for fat. But how does the burning of food translate into ATP bonds that we use for energy in vivo? just curious thanks

There is a contradiction here. If the mitochondria is energy efficient, then the aerobic regimen should provide maximum power. However, high-intensity exercise provided by an anaerobic regimen based on the Cori cycle

Please help. I could not find the cause except for a few specific diseases. Thank you

Hello, sorry if this isn't the appropriate place to post this but I need help. First of all, I am not a biochemist, but I need one. You guys are superheroes, I hope we can make this work! I've been into researching male pattern hair loss for nearly a decade now,, I have a good enough understanding of the steps of it, the most major one being the overproduction of the DHT on the scalp tissue and the prostaglandin respond it creates. There's a very promising in-vitro study with zinc, b6 and azelaic acid. This has been formulated into a DIY topical called Zix 20 years ago and it works fairly well as a natural product. That being said, the in-vitro studies has …

I hear of dispute on the nutritional value of protein. Some say it's good for you and helps sustain sugars and starches for longer periods of time; others say it inhibits calcium absportion and Google suggests the truth is if nothing else in the same general direction. If the issue were the need to sustain sugars and starches for longer periods of time, would it be comparably healthy to eat sugars and starches in more frequently, but in smaller portions, such that the sugars and starches are sustained by the fact that you're eating them a little bit at a time?

I have data of a cell migration assay that I need to evaluate. I have a number of replicates for each condition and the total number (#) of migrated cells for each replicate and condition (control vs treatment with different substances). Now, there are various ways to do the evaluation - I could do the following comparisons: * total # of migrated cells under the different conditions * % of migrated cells relative to total # of seeded cells * % increase in # of migrated cells vs control mean * fold change of # of migrated cells vs control mean Which type of evaluation would you favor and why?

My friend says that the vitamin B12 produced as a food supplement is harvested from animals. I pointed out that online articles indicate that B12 is produced through fermentation. He claims that the strains used for fermentation get "old" and have to be replaced by new ones harvested from animals. I'm skeptical about that. Who's right? If you could provide sources so I could prove it to my friend (assuming I'm right), I would appreciate it. Thanks.

What's the safest way to preserve a small amount of phospholipids that were (rather crudely) extracted from chicken egg yolks? I find that new learners in biochemistry are always more interested when they can see materials in their physical form rather than a structure on paper before an experiment is done, so I've decided to explore the idea of collecting biological chemicals. I'm thinking that I could use a little bit of solvent or preservative in a glass vial, but that's about all I've got so far. What materials could you use to do this, and how long would it last before any undesired effects happen? If possible, I might collect multiple samples and store them in …

Hi all, we are thinking of upgrading our gDNA quantification method from the agarose gel method to the NanoDrop. I've been trying to research online what quantification methods are best for DNA extracted from plant tissues, including wood and decayed wood, because I know these extracts can introduce unique contaminants that would not be present in something like blood, for example. I haven't found much information online about this topic specifically, just lots of discussions about NanoDrop vs. Qubit vs. qPCR quantification, etc. I think the NanoDrop will be a good move for us but I was wondering if anyone out there with expertise in plant DNA extracts has a favorite quan…

I found an article on this via twitter which is at https://cen.acs.org/physical-chemistry/Astatine-chemistry-puzzle-shows-anticancer/98/i31?utm_source=Twitter&utm_medium=Social&utm_campaign=CEN I am just interested in science here so tried to build the molecule from molecular model kit. I am not sure if I have built it correctly though, mostly between the C6H4At – and N2 portion, i have had to put a carbon to allow for the bend so assumed that is taken up with a carbon and perhaps 2 hydrogens, (based on my limited understanding of this) there is a molecule diagram on the above website, I made a video (granted not very good with a webcam) of my effort…

Hi, I wanted to ask if anybody has extracted DNA from dried leaf samples that were stored at 4 Celsius. I know that DNA is quite stable in solution after extraction, but I don't know about the tissue. I'm assuming there will be degradation. But in any case I thought to ask if anybody has ever tried, before throwing the samples... Thanks

Hello I'm trying to calculate eGFR using the CKD-EPI formula but my results are wrong and don't know why. I use this site to check my results: https://qxmd.com/calculate/calculator_251/egfr-using-ckd-epi And I use this site for a very simple formula: https://www.niddk.nih.gov/health-information/professionals/clinical-tools-patient-management/kidney-disease/laboratory-evaluation/glomerular-filtration-rate/estimating I'm testing on a white male 40 years of age and with serum Creatine 51 so the formula is: GFR = 141 × (Scr/0.9)-1.209 × (0.993)Age I calculated in many ways, this test is done directly at Pos…

Hi Guys- have a question , I’m trying to clone guide RNAs into a vector with Bfu1 sites. I was told to make an oligo that has a protospacer sequence- bfuSite/forward- reverse bfu1 site. the protospacer is the same as the forward guide sequence without the Bfu1 site i don’t understand what the protospacer is???

Dear friends, I would like to ask whether someone acquainted with Vectastain ABC-AP immunostaining kit. I would like to use this kit for my research but I am confused with catalog numbers of the kit. I would like to buy full kit for convenient using, so whether I choose AK-5002 or AK-5002-NB as it seems that 2 kits contain similar components? Thanks! Have a good time!

I know you guys is smart enough to know the answers and I know how generalized this sounds. But my girl is dying from cancer rapidly. Her hair is gone and it's spreading and she doesn't have time left. I know it's a longshot and I know she might die or we might die before it even happens if it happens but I want to know how can we acheive immortality or at least reversing the aging process. Telomeres I heard is the key. If our cells keep destroying themselves then there has to be a fucking way to stop them from destroying themselves. Have no one tried testing this shit on rats or mice and tried to do a DNA reversal. I don't understand why nothing is happening but we're su…

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Hello all, Me and my supervisor have developed a new way to purify proteins. It's an affinity based method that used magnetic nanoparticles. We have been undertaking some market research to see at what scale this approach would be the most sensible to implement (e.g. large scale enzyme purification, medium scale high value biological production for pharmaceuticals or small-scale academic/RnD environments). If I told you that we can: purify the proteins to the same degree or better than your current affinity purification system produce same or higher yields produce the proteins without affecting their native conformation (quality control) …

Hello! I've recently started studying mostly fungi, but a little bit about bacteria as well - I got some agar plates (Potato dextrose) to play around with. I know to store them media side down, but 2 of the plates got flipped over accidentally & the lid shifted so it left the agar exposed to the air for a little while. As a result, there was some growth happening on these exposed plates and I was wondering if anyone might be able to help identify what I'm seeing? I know a lot for the short time I've been researching, but I'm still really new to this! I have tried to take decent pictures of the plates but I have them sealed up in a bag so I'm not breathing that in, and…

Hi everyone! One of the steps of my research project requires delivery of miRNA to the hCMEC/D3 cell line. I've read that naked miRNAs are poorly taken up in vitro due to their negative charge - can someone suggest delivery methods (along with protocols) that overcome this issue? Any advice would be greatly appreciated! Thanks!

Hi everyone! I'm using the hCMEC/D3 cell line to mimic BBB endothelial cells but am not sure how to create MS-like inflammation in vitro. Is it possible to do so? If so, please suggest any models you are aware of. Any advice would be greatly appreciated! Thanks!

Hi all, I'm working on a project on multiple sclerosis and am having a difficult time finding an immortalized B cell line that mimics the b-cells that infiltrate the BBB in multiple sclerosis. Can anyone suggest a cell line? Thanks!

Anyone know the difference between molecular dynamic simulations and normal mode analysis am a bit confused of how they both differ in terms of what each tells you.

I'm a Med Student who is learning coding Matlab for bioinformatics. I would need to refresh the molecular biology concepts to understand any problem or situation that I encounter with with regards to analyzing experimental biological data through bioinformatics. Could anybody please recommend me a good, clear and concise source (pdf, webpage, etc) to review molecular biology concepts overall? So thankful!!!!