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LukasJ

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  1. Thanks for the thorough answer. Definitely validated some of my assumptions. I can't say at this point what the savings would exactly be per sample. This sort of validates what I was thinking. In principle there is not resistance to new techniques, but it needs to proven to be worth it. Perhaps a focus should be on groups that already use magnetic resins as they are already benefiting from the ease of use enabled my magnetic collection. I wonder if hypothetically we had a product that really does match/exceed quality and purity expectations of the end product, how would one go about in communicating that. Would it be thorough documentation in publications? Trade-show demonstrations and lab visits? Shouldn't be any more issues than when using other affinity techniques. A desalting step (or even just a dilution as the eluent concentration is lower than Ni-NTA.
  2. Hello all, Me and my supervisor have developed a new way to purify proteins. It's an affinity based method that used magnetic nanoparticles. We have been undertaking some market research to see at what scale this approach would be the most sensible to implement (e.g. large scale enzyme purification, medium scale high value biological production for pharmaceuticals or small-scale academic/RnD environments). If I told you that we can: purify the proteins to the same degree or better than your current affinity purification system produce same or higher yields produce the proteins without affecting their native conformation (quality control) achieve this at a fraction of the cost (say compared to Ni-NTA resin and especially to magnetic Ni-NTA beads) would you consider switching, or at least trying this method? The only caveat is that you would need to clone your proteins to include our tag (comparable length to standard tags) We are basically trying to understand if it is feasible to expect protein purification experts to buy a product that expects them to change the tags that they are familiar with. Let me know if you have any questions. It would be cool to have a chat!
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