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newmanreb

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About newmanreb

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  • Favorite Area of Science
    Molecular Biology
  1. Thank you CharonY! This helps me out a lot. As for the probe that binds to the target region, how long in bp would that tend to be? I suppose I assumed that the probes would be about the same length as primers, and although primers specifically bind to the target, they are clearly not enough to determine the identity sequence. However, they may be thinking of universal primers used to amplify the 16S region, for example, which are not designed to be species-specific and therefore not intended to identify the sequence.
  2. Hi all, I'm doing some research at my company about implementing qPCR, because we are still in the dark ages with conventional PCR (cPCR). I came across this information in a publication, and I haven't been able to find more help online to clarify what the authors are saying. I was hoping someone here could illuminate things for me. This is a quote from a 2002 paper, citation below. It says, "A major reason why classical PCR has not been adopted by most plant disease regulatory and diagnostic laboratories is the time and labor required to confirm the identification of the PCR amplifica
  3. Hi all, we are thinking of upgrading our gDNA quantification method from the agarose gel method to the NanoDrop. I've been trying to research online what quantification methods are best for DNA extracted from plant tissues, including wood and decayed wood, because I know these extracts can introduce unique contaminants that would not be present in something like blood, for example. I haven't found much information online about this topic specifically, just lots of discussions about NanoDrop vs. Qubit vs. qPCR quantification, etc. I think the NanoDrop will be a good move for us but I was wonder
  4. Hi there! I am with a company who is lucky enough to be expanding our operations into a new building. Our PCR laboratory will feature 3 rooms: A reagent prep room, a sample preparation room (DNA extraction) and a post-PCR laboratory. Since we are designing the building, we get to decide what air flow we need throughout this unidirectional workflow. Now. It's clear to me that the reagent prep room, the "cleanest room", needs positive pressure to keep contaminants out. Also clear - the post-PCR room needs negative pressure to keep amplicon contaminants IN. What is not clear to me is how to
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