Genetics

Hi! I was wondering if any one knows how to find the location of a promoter region from a transcription start site? I'm specifically looking for the AR (androgen receptor) promoter region. For example, if it's -5000 to +100 bp relative to the the TSS. I've been using the Eukaryotic Promoter Database (https://epd.epfl.ch/cgi-bin/get_doc?db=hgEpdNew&format=genome&entry=AR_1) but am not sure what to define the sequence position/how to find that. Any suggestions would be appreciated, thanks in advance:) (sorry I think this should be under the Genetics section)

I am learning about cre-loxp recently at this is all new to me. I want to have a mouse strain that will have a tissue specific knockout of a certain gene. Is there a way to introduce a reporter in that same tissue? For example, if I have a liver specific cre and I want the liver tissue to express lacz after knocking out the mitochondrial transcription factor from liver cells with floxed strains, what should be my approach?

Is there an easy way on Ensembl or other resource to find out whether a SNP alters a amino acid and / or protein? e.g a missense variant may change the codon but this could code for the same amino acid

Hi. I would like to ask, has there been attempt at erasing imprinting done on a chromosome, for instance by cultivating them in bacteria or a different genetic system, or individual... or merely cells cultural of the same organism ? That way we could get mono-parental disomy to be viable, since the only reason they don't (I think NO such mouse develops, right ?), is imprinting. I am a student, or researcher... whatever that means, interested in consanguinity, its effect, beyond the usual "recessive" mantra. I figured out that the simplest, to reveal every "defect" or considered as such regards to functionality, would first be this: - father gives an ab…

I have a simple question. Can a virus be sequenced if it hasn't been isolated? If yes, could you please tell me how it's done?

Hi, I'm quite new at this and now I have some data to represent but I'm not sure of which is the best way. Could you please help me? I have 12 lineages with different quantity of sequences analysed in each one. I also have a list of genes (200) to check if these genes have deletions on these sequences. These genes are subdivided in groups of gene function. So my results are separated by lineage. Example: For lineage 1, the gene 1 (virulence gene) is deleted in 60% of sequences, the gene 2 (virulence gene) is deleted in 0% of the sequences, the gene 3 (cell wall gene) is deleted in 50% of the sequences, the gene 4 (cell wall gene) is deleted in 25% of the se…

I was thinking about my personal top four smartest people of all time. In chronological order; Isaac Newton, James Clerk Maxwell, Michael Faraday, and Albert Einstein. Unfortunately only one of them had children. Would it be possible to exhume the remains of these geniuses and sequence their DNA so that we could clone them?

Hello, I have some doubts about genetics that I had during my studies. Considering the sequence ACGCGT, what does it indicate? A splicing site, a restriction site? The other part is: If I have the virus B. Subtilis, what is true about the transcription of his genes? I did not understand if it uses or not the polymerase of bacteria and If it uses or not sigma70 for transcription of early genes. Also what it is not really clear to me is if it uses or not sigma of bacteria and if it uses or not viral sigmas to transcribe intermediate genes. I know that are maybe difficult but I am pretty confused. About the question relate…

Hey everyone its AgentF2S So, I decided to try and make a Pokémon using genetic engineering (in my mind ofc) u can suggest and and make ur own too! Mine is Pikachu and I decided I will use multiple DNAs So my starting point will be a bunny embrio, I will inject it with golden bat's yellow fur dna somehow get it red cheecks the Idea for the electricity is it will have mini electrostatic charge generators.... Hundreds of them on each cheeck, so that will be using multiple mini muscles to make furry hundreds of fuzzy balls to rub together, but I have to figure out a way to make pikachu's body an insulator... Idk how, I'm open to suggestions Nothing else is nee…

I'm interested in expansion of this definition, specifically the difference between anatomic and segregational relationships. syn·te·ny. (sin'tĕ-nē) The relationship between two genetic loci (not genes) represented on the same chromosomal pair or (for haploid chromosomes) on the same chromosome; an anatomic rather than a segregational relationship thanks

What are some specific examples of gene duplication giving rise to new beneficial traits in organisms?

Hi. I’m new here and am a native Software Engineer with a M.S. in Computer Science. I’v grown an interest Recently I’ve grown an interest in Bioinformatics however. There is an internship that I really want to get. But that’s beside the point. My question (I have a lot) has to do with petri dishes. If I run a wire with a charge, grounded, through the fluid in the petri dish is it possible to 1) detect the EM frequency of any other things in the petri dish using an oscilloscope Thanks Ted

Hello everyone I have a genetics related question on how to achieve a transformation in agrobacterium without having the gene being expressed bu the agrobacterium. According to what I have learned it seems relatively simple to achieve a transient transfection with plasmid vectors and cationic liposomes in bacteria. However my concern is that this approach will result in the agrobacterium expressing the gene instead of adding the gene to it's Ti plasmid. The goal is to insert a firefly luciferase gene into Arabidopsis.

If a particular trait is determined by having at least three mutations on six involved genes and this trait provides a strong survival advantage over those that don‘t have it - how long will it take ROUGHLY till the trait has for example doubled in a given population? I mean: Are we talking more of several generations or several hundred or even thousands of generations? Is there any kind of online calculator to solve that kind of problem?

Hi all, I wonder what your all think about all the following situation. We have a son who was born with a de novo (spontaneous) mutation for DMD (Duchenne's muscular dystrophy). There is no family history of this disease on either side, plus my wife was tested and came out negative as a carrier. Unfortunately a week/two weeks after conception, my wife ended up visiting her mother in hospital, she was getting a cancerous lump in her leg removed and part of the treatment was with a radioactive isotope. On the first visit (I wasn't there), the specialist told her and my father in law of the precautions, one of which is if you are possibly pregnant then there should…

My RNA concentrations from brain tissue were at highest around 80ng/mL and as low as 5ng/mL. Can I converse my RNA to cDNA with this low concentration of RNA?

Hello everyone I'd like to learn more about calli and tissue culture in general, my goal is to grow an arabidopsis callus so that I can hopefully insert a gene into it. If anybody has resources related to growing arabidopsis calli please let me know. Additionally if you guys have recommendations for suitable growth medium also let me know. Thank you

Pardon for my ignorance. Does the new field of epigenetics show that all humans have the same intelligence?? That is, all humans have same genes related to intelligence (hence all humans are exactly equal in intelligence) but those genes are switched on and off by epigenetic processes. So, the the IQ genes were switched on by epigenetics for people who migrated to cold climates increasing their IQs, but were switched off by epigenetic processes for people in the tropics thus making it low. Has this been shown in epigenetics research??

My husband and I have always had straight hair. We had IVF and now have a beautiful 15 month old girl. She had straight hair until the last few months or so whereby she has wavy curly hair at the back but mostly straight elsewhere. Curious to know why she currently has this type of natural style going on we do have one or two members on both sides of the family who have had/do have some curls going on-not the grandparents we don't think. Have we passed down some sort of dominant hair Gene's but display differently in our girl or has a spontaneous trait occurred? Would love to know more thank you.

why phage DNA molecue is not maintained stable condition in host cell?

I once saw a diagram in my science textbook vaguely explaining how advanced genetics can inject a gene for insulin production into the 'plasmid' (not sure how it's spelled) of a bacterium, allowing it to produce insulin and produce offspring with the insulin gene. Is this real? And if so, how is it implemented in the real world and what problems could pop up with it Thanks in advance!😁

Hello everyone I'm currently a student so apologies in advance if I'm incorrect or describe what I'm trying to do poorly, but essentially I'm kind of confused on how extracted mice embryo are fertilized and genetically modified during in vitro fertilization. To be a little more specific consider the following situation and again if I'm wrong about anything below please correct me: 1. A pre-pregant female mouse is euthanized and then dissected to retrieve it's embryo. 2. A mammalian plasmid vector such as pcDNA3 with a cationic liposome coating is added to the embryo so the embryonic cell can uptake the plasmid DNA. One of the things I'm confused about is how t…

Aneuploidy is when there is an abnormal number of chromosomes in a haploid set. It can be caused by A: meiotic errors during gamete formation B: mitotic errors in the post-zygotic period. But how can you distinguish if the aneuploidy is caused by A or B??

I'm currently a student learning about genetic engineering and one thing that I didn't quite understand is before extracting an egg cell from a female, the female mouse must first perform coitus with a vasectomized male mouse. Does anybody know why or how this is related to the female mouse releasing an egg cell?