Microbiology and Immunology

Yes, you read that right. A stool culture was s/o to xld and mac. The colonies are buried into the xld and mac agar after 24 hours. Colonies are dug out and g/s. The g/s = GNB. They are lactose fermenters too. They look like little beads in the agar. Dug those out and s/o to a bap/mac bi plate, and chocolate. The GN broth at 24 hours was s/o to a fresh xld and mac agar. Today there is ng on the Chochlate, Bi plate and xld/mac (from gn broth). Dug more colonies out of the primary plates and inoculated then into a Thio broth and a TSB. Re incubated all plates and put the Chocolate one in a CO2 incubator. Has anyone encounter this before? Will post …

Can anyone help me to explain what does anti -bovine monoclonal antibodies mean? Am I right thinking, this is a group of specific antibodies that fight against particular antigen, that are present in bovine blood? thanks for help?

I wonder, microbes are very weak organisms, and every microbe can be affected, if not disintegrated, by thousands of chemicals [different types of attack], so, why till now there are many incurable infectious diseases?

Read here http://www.winnipegfreepress.com/arts-and-life/life/style/seniors-at-risk-of-catching-infections-in-emergency-departments-study-137895558.html How can this happen in today in this time with top standards ? I can see 100 or 150 years ago but not today.

http://topdocumentaryfilms.com/phages-the-virus-that-cures/ Very interesting doco. From my university days I am well aware of bacteriophages, but this documentary details serious US efforts to develop bacteriophages into high standard and practical treatments for bacterial infections in the face of inexorable rise of antibiotic resistance. There will be one limitation however that will be very difficult to overcome. And that is bacteriophages cannot be injected into the blood stream as they are soon destroyed by our own immune response. Hence they are likely to be limited to gastrointestinal, respiratory, wound and topical applications.

Generally Laminar air flow chambers are used for Microbial culturing, there is any method to do sterile culturing in the absence of LAF?

Dear All , A very Happy Darwin Day to all.On this day I would like all of you to introduce with the fascinating world of Darwinian or Evolutionary Medicine. Evolutionary Medicine is all about understanding the evolutionary origin of diseases , understanding the selection forces that have shaped those traits.The field is emerging like anything and due to the constant efforts of the founders evolution will soon be part of Medical Curriculum in USA.I would like to share my brief write up on Evolutionary Medicine with you all : INTRODUCTION: Darwinian Medicine which is the synthesis of two different disciplines of Evolutionary Biology and Medicine is emerg…

I had an idea for how to make cellulose digesting bacteria which could possibly be used in the third world in order to create a limitless(virtually) food supply. The idea was to grow a huge quantity of cow intestinal bacteria which possesses the cellulase enzyme complex, and grow a huge quantity of human intestinal bacteria, and keep them seperate. Then take some of the cow intestinal bacteria, kill it, then mix human intestinal bacteria in with the dead cow intestinal bacteria and add cellulose containing products(wood chips, grass ect...). What I'm thinking is that the bacteria will collect DNA from the cow intestinal bacteria, and by chance some of that …

Im currently reading the book "The China Study" and want to do one of my reports on it for one of my classes how ever the book makes some pretty bold statements stating that almost all autoimmune diseases are caused by diets high in proteins more specifically animal proteins, cancers are controllable with a low protein diet example rats fed aflatoxin were given different diets of varying amounts of protein the group at 5% protein did not develop any cancer vs 20% protein had a 100% cancer rate, and (this one i need explained) that animal proteins increase blood acidity and the body in order to neutralize the high acidity takes Ca from the bones leading to things such as o…

In the book I'm writing a there is group of researchers who are trying to cure a pandemic disease. For plot reasons the cure has to be a retrovirus. My problem is how to administer this retrovirus. My first thought was injection, but due to the setting obtaining a reliable supply of clean needles would be nearly impossible. So, now I'm thinking some kind of concentrated drops administered orally/sublingually. Could this be a plausible means of transmitting a retrovirus? If not would eye drops or nasal drops be any better?

In the identification chart given by Bergey's manual, in the flowchart for the identification of Bacillus spp. there is a test written as swollen cell (containing spore) for voges-proskaeur test negative organisms? I wanted to know the test principle and procedure. Thank you.

Hi: Thank you for answering my question. My question is concerning the efflux pump and MDR 1/ I was under the belief that efflux pumps were specific to biofilm formation, if the pathogen is not a biofilm related organism, can efflux pumps still exist? 2/ Are efflux pumps the main obstacle in multi drug resistance? 3/ Does anyone have any resources on natural or pharma efflux pump disablers? Thanks for your insight!

Can someone find out the mechanism by which macrolides cause hepatotoxicity in detail?

I have some questions regarding varicela virus. I have checked Internet but the information varies so much. If anybody knows that: What is the chance to get infected with this virus for adult during the close contact with infected one ? For children it is written 97-100% but I didn't find any information for adults. Thank you.

Hello, 1.Do you know if Salmonella, E-coli and others can survive in freezing temprature of -18C ? 2.Can they survive in dogs stomach acid in PH level of 1? Thanks!

Hi: 'Salmonella fluid' is a theoretical liquid that consists solely of pure water and live cells of bacteria in the Salmonella genus. This liquid has the same viscosity of healthy human blood and contains an equal amount of each and every species, subspecies, and serotypes of Salmonella. This high viscosity is due to the large concentration of bacterial cells relative to the water. Let's say I were to go 4 days [96 hours] without eating/drinking anything other than this fluid. In this scenario, for 4 days, I eat five small meals consisting solely of 'salmonella fluid'. The first meal of the day is the biggest while the last meal of the day is the smallest. The 2nd…

Dear, I have been given a topic to find out the "Effects of different containers on the Microbiological Quality of Bottled and Fresh water". Can you guide me and tell me what are the microbial parameters that i need to consider while finding the effects of containers, Also tell me, how do we perform the Heterotrophic Plate count and APC count on the water, can you give me the link to some papers for this?, i would like to know different materials and method required to find to run the HPC count in the water Waiting for your Kind reply.. Regards Basra

If a mother is vaccinated for a virus during or shortly before pregnancy (the vaccine used is a live attenuated virus).. And many many years later, her son is vaccinated for that same virus... Why would he not be responsive?

Hello, I'm currently working on a 3D conceptual rendering of a sperm cell and I found that most illustrations that are out there show the thickening behind the 'head' as a bunch of toruses, sometimes a thick spiral. From schematic cross-sections I know it supposed to be mitochondria and to my simple mind those suppose to be a bean/capsule shaped objects. So are they just very closely wound around the center ? Also amazingly 99% percent of the illustrations get the proportions completely wrong (the "head" is a different shape, the thickening in question is exaggerated, very often the 'head' is symmetrical as in fact it's flat ... etc). I know that…

Please give me some help in that discipline, I want the name of authors, titles book etc. What is the best book about geomicrobiology?

hello to all. i have a curious eye problem which im hoping someone might help or advise me with. on certain backgrounds ie; a bright sunny day,foggy day,or even while im looking at my computer screen typing this,i can see what i can best describe as parasites swimming in my vision. they,re not floaters because they actually move or swim about and theres a glint off them as they move. i cant see them all the time and they dont effect my day to day routines. when i go to sleep at night though they appear when i shut my eyes for a few moments and thats it basically. if anyone has an idea to what they are or if it might lead to something more serious,i would be very grateful …

S.epidermidis is a gram-positive pathogen that eats away connective tissues and walls of blood vessels from the outside and then inward. Wounds caused by S.epidermidis do not coagulate, clot or harden. S.epidermidis dissolves connective tissues and the walls of blood vessels instantly. S.epidermidis is also a lethal anticoagulant. It does not prevent clotting initially but liquefies any clots that have formed. Platelets in the wounds are allowed to go through the chemical reaction that would clot blood, however these clots dissolve right after they are formed. Here are the characteristics of a wound caused by S.epidermidis: 1. The epidermal, papillary-…

Hi: What is the smallest bacterium that fits all of the following categories?: 1. Not gram-negative 2. Free of lipopolysaccharide 3. Coagulase-negative 4. Relies solely on homolactate fermentation for energy and therefore does not generate any CO2. 5. Aerotolerant-anaerobic [can survive in oxygen but doesn't use it for respiration or otherwise require it]. 6. Hemolysin-negative 7. Free of super-antigens 8. Non-allergenic Please note this is not a homework question but simply a question of my genuine interest. Thanks a bunch, GX

What nutrients and bodyhacks (exogenous chemicals or, to use the word liberally, yoga) boost neutrophil counts? Why can't I type 'neutrophil doping' into WolframA and get protocols for boosting neutrophil concentrations? I blame British empiricism for the retarded sQuo in spite of the Enlightenment philosophers' victory at the end of the Dark Ages and the level of science at the beginning of the Cold War.

Hello fellow scientists, I have been having a slight problem with protein solubility, when i overexpress my protein of interest (14kda) using LB (luria bertani) broth, my protein is soluble and i am able to purify however when i do the same overexpression using minimal media (M9) the protein is insoluble, what kind of variations either to the media or growth conditions would anyone recommend in order to get this protein soluble, i plan to do NMR to resolve the full protein structure, any feedback would be most appreciated!