Microbiology and Immunology

I was going to get a mircoscope and I was searching on ebay. I found a Amscope that sold superizingly cheap microscope and I was wondering if anyone bought a microscope from them and how relayiable they are and if it is worth buying? Site: http://www.amscope.com/ Product: http://store.amscope.com/b100b-ms.html

We have discussed food proteins in injected vaccines and their association with the development of food allergies. http://www.sciencefo...food-allergies/ http://www.sciencefo...?hl=polysorbate http://foodallergyca...allergy-causes/ We have discussed pollen proteins injected by insects and their association with the development of pollen allergy. http://www.sciencefo...eatment-plants/ We have also discussed the possibility that skin/muscle proteins injected by intramuscular shots resulting in allergies, autism or autoimmune disorders. http://www.scienceforums.net/topic/85502-vaccines-allergies-autism-and-autoimmune-disorders/ Flucelvax is an influenza vaccine …

We have discussed food proteins in injected vaccines and their association with the development of food allergies. http://www.scienceforums.net/topic/78023-direct-evidence-from-the-cdc-that-vaccines-cause-food-allergies/ http://www.scienceforums.net/topic/83698-polysorbate-80-vaccines-and-federal-allergen-regulation/?hl=polysorbate http://foodallergycauses.wordpress.com/category/food-allergy-causes/ We have also discussed pollen proteins injected by insects and their association with the development of pollen allergy. http://www.scienceforums.net/topic/80120-pollen-allergies-related-to-insect-bites-and-water-treatment-plants/ It is the flu season and I have been researc…

Is it necessary to discard a given amount of sample from the last tube while doing serial dilution? Why is it done in some cases?

Hi, everyone! A new guy here. Do you know NHP Research? As far as I know, NHP are as follows: Macaca mulatta (rhesus monkey), M. fascicularis (cynomolgus monkey), Macaca arctoides (stump-tailed monkey) and several other primate animal species for specialized biomedical applications. In addition, there are also non-human primates bio-research studies including: Proof-of-Concept Studies Challenge Studies Efficacy Studies Potency Studies Therapeutics Vaccines Antiretrovirals mmunological and Virological Assays Interested in this topic? Do you know anything about it? Share with us! Do U have any questions about it? Feel free to ask me! Hope to disc…

Immunohistochemistry (IHC) is a compound name of three parts: “immuno” refers to antibodies and antigens based, “histo” meaning tissue, and “chemistry” referring to chemical substances reactions. Immunohistochemistry detects antigens in cells of a tissue section by using the idea of antibody binding specifically to antigen. Immunohistochemistry is a variation of histochemistry which is a technique that reveals tissue structure and abnormalities but the stain does not distinguish among proteins. In 1941, Dr. Albert Coons, a pathologist and immunologist physician, was the first person who began to do IHC in collaboration with Louis Fieser, an organic chemist. They succeede…

Copy pasta removed To read more on this topic visit this website Link removed If anyone has more interesting topics related to this please let me know. Thank you

Popular game pandemic has conquered the iPhone market. Owned by many people, the game's point is in essence to kill every last human being on earth...but would a disease actually do this? To start, a disease is really only ever as deadly as it needs to be. Virulence, the level of how deadly a disease is is primarily determined by how much a disease needs or doesn't need to spread. The cold for example, has little to no side-effects because it's very easy to spread and gains nothing from hurting you. Something like Ebola would be on the opposite end of the scale. So in theory, if the cold was given to everyone, would it become harmless? Evidence suggests that such has happ…

Hi all, I've been working with FACS analysis for a while. For evaluating my results I need some literature (books, publications) to get an impression of immune cell distribution such as CD4+ Cells in lymph nodes. Some ideas? Thanks for helping me! Best, Immu

Hi all, this may be a naive question. I saw papers using cholera toxin to infect mice to induce antigen specific responses by T cells. Does anybody know if cholera toxin is dangerous or not? Does it infect human? How do I handle it? It's available from sigma, so I assume it shouldn't be dangerous. But just want to make sure. Thanks!!

Can anybody give me a rought estimate of the concentration of IgG in normal mouse serum? Thank you.

I'm not a scientist, but a lowly physician untrained in the lab skills needed to maintain a bacterial culture. I do home-brew beer and maintain yeast cultures... but I'm not sure how the two might relate. The reason I'm posting is because I recently purchased some AObiome "cosmaceutical" mist, and I'd like to see if I can maintain the culture so s month's supply lasts a full year or more. It's very expensive at $99/bottle, so if the stuff works as intended, maintaing a culture will be cost effective. If this were a yeast culture for brewing, I would just use some and then top up the bottle with nutrient mix (wort), and wait. Would it be this simple for a bot…

Polysorbate 80 contains oleic acid derived from vegetable oils. So it is not possible to guarantee that it is free of allergen proteins. For example, NOF Corporation claims "number one quality product in the world" for its "injectable grade" Polysorbate 80 HX2 product but it is not allergen free. http://polysorbate.jp/ Federal regulations below prohibit the addition of extraneous protein known to be capable of producing allergenic effects into injectable vaccines. http://www.accessdata.fda.gov/scripts/cdrh/cfdocs/cfcfr/CFRSearch.cfm?fr=610.15 Title 21 of the Code of Federal Regulations (CFR) Section 610.15 Constituent materials. These regulations state …

Hi! Has anyone grown E.coli K-12 under anaerobic conditions using ethanolamine as the sole nitrogen source or even carbon and nitrogen source with cobalamin? E.coli has the enzyme ethanolamine ammonia lyase which requires cobalamin (B12) to break it down to produce ammonia and acetate. I have successfully grown E.coli K-12 in ethanolamine (N source) + glycerol (C source) with added B12 under aerobic conditions. I have also grown E.coli K-12 in ethanolamine (C and N source) with added B12 under aerobic conditions. But when I try to grow E.coli K-12 anaerobically with ethanolamine + glycerol, it does not grow. I realize this has something to do with the electro…

Hi folks, I'm new to the field of microbiology and have a few questions. What are the parameters and "special techniques" than can be changed/used to grow Streptomyces? My goal is to grow Streptomyces to produce a certain product which it normally doesnt produce. The only things i came up with were the variation of media/time and the conjugation with a plasmide that codes for regulatory proteins. Can you help me ? thanks in advance

I 'm searching for good scientific information on this topic : "the interaction between human Norovirus and gastrointestinal microbial environment". I don't find enough relevant articles on the free databases on internet. Can someone advise me some good & free databases or send me good articles ? + is there some software to organise articles in order to save all the articles and sites I found and to make it easier to make the acknowledgement when writing a paper? Thanks a lot! (english is not my native english.. but i'm working on it. the articles have to be in english.)

Hello I want to prepare some new antibacterial ointments, but i can not find an assay/protocol for checking ointments. ?How do the industry or the academy check the antimicrobial activity of ointments ? TNX

hello friends, just a simple Q, could anyone tell me what the maximum daily intake for pumpkin & and any side effects ?? (i don't want to see my skin turn into pumpkin) after google for 2 days, i cannot get a reliable answer. thanks.

How to calculate sensitivity and specificity of the virus neutralization test (incubation period 1h/37 and 24h/37? Positive and negative samples : 15/20 in first test (1h/37) and 22/13 in second test (24h/37). I need to compare these two test

why do allergies exist?

I know that HIV is a virus that attacks the white blood cells making other bacterias able to capatalize. But isn't the job of white blood cells is to kill viruses and bacterias. Why doesn't the white blood cell just attack and kill the HIV virus?

Has anyone worked with fast-growth bacteria in the lab (batch culture or chemostat). I will be grateful for any insights on your own scientific experiences. For more information, see this article: link removed Thanks

Hi, Is a UTI the same thing as a urogenital tract infection? Glowstar

Question 155 says An aspirate of a deep wound was plated on a blood agar plates aerobically &anaerobically. At 24hrs there was growth on the anaerobic plate only. the next step is: begin organism identification The answer is to begin organism ID. Why would we not let the plates continue growing? (I hope this is not a very obvious Q.) Glowstar

Hi everybody! I have a question. What's the difference between serotype and serovar, in relation to the Salmonella genus? I am doing a HW where I'm supposed to find a test that is able to differentiate between Salmonella enterica typhi and Salmonella enterica Enteriditis. So far, I discovered that the Kauffman-White method is the gold standard. However, there is a drawback (from the website below): "The problem with this conventional method is that it is laborious, time consuming, and cannot differentiate within serovars." Does this mean that by using KW, I cannot distinguish between Salmonella enterica typhi and Salmonella enterica Enteriditis? Glowsta…