Biochemistry and Molecular Biology

Hi, people. Wondering if the highly touted "drinking hydrogen peroxide" actually adds any usable oxygen for our body tissues. A friend of mine loaned me the book: hydrogen peroxide scam I started reading and while it has some interesting reading, it is touting this peroxide drink as a cure all, and reeks of theytypical scam publication, with no footnotes, no credentials of the author, no nothing science publications usually have. I started thinking, hmmmm, they say to add 3 drops of 35% H2O2 in a glass of H2O. Man, that cant amount to a whole lot of O2 even if it was O2 and not just atomic oxygen, which, I believe, is NOT usable in our tissues. Even if you dra…

Hello all, I was doing some reading on the chemical reactions responsible for feelings of love and discovered that the primary chemicals responsible were Oxytocin, Vasopressin, and Dopamine. What I was wondering is if, if one were to find the right composition of chemical agents, one would be able to neutralize or at least minimize the effects of said chemical reactions. Thoughts?

I'm reading about the neurophysiology of eating, and I considered a few things. 1: 2: As such, here is my main question: What molecules can be used to inhibit insulin? Here are the questions that revolve around that question: 1. If a molecule were used to inhibit insulin, then this would this inhibition induce the body to breakdown fat? 2. How safe is such a proposed idea?

Fish have to be placed on ice asap after caught. I remember if not, an enzyme/whatever starts converting stuff into stinks like the famous amines. Anyone know the details/correct explanation? Thanks!

Dears Friends scientists and specialists .. Please I need some help by information .. Is there a supplements or medications can restore or fix the mitochondrial damage ? I know about the supplement Q10 , but I want to know if there is any thing else or more effective .

Hello, a friend of mine (a Young Earth Creationist:doh:) is trying to convert me and tell me that motor proteins are irreducibly complex, and I don't know enough about it to refute him (I am not really much of a science guy.) He says that the kinesins move along microtubule cables, and that it couldn't have come about by evolution. Can someone explain to me how this thing evolved so I can shut him up? He keeps insisting that since I cannot explain this, that evolution is my religion, and I am getting tired of it. Thanks.

After amplification of the intergenic spacer region (IGS) of the ribosomal DNA from nematode species by PCR, gel electrophoresis reveals multiple bands. Why is this ? Here is some information that may be relevant. Depending on the species four to five bands can be observed which can have different intensities. The top band is usually the most intense The primers used are designed based on the conserved regions of the flanking 18S and 28S. Stringent PCR conditions of 60 C annealing temp and low MgCl conc employed to reduce non specific amplification. The target is part of the rDNA which is a tandemly repeated unit. I have two lines of thought on why this …

Will a specific antigen initiate the response of a B and T cell so there are receptors for the same antigen in both the humoral and cell-mediated response?

Is it possible to grow wood cells in bioreactor and print three dimensional mass with it ? Some researchers are talking about printing human at the future , his organs now. When you print human cells , cells tend to stick each other. Is it same for greens ? May be it would be possible to print musical instrument , than kill it , soak in acid and crystalise it. What do you think ? Which forum , list help me to find the answer if this is not ? Thank you , Mustafa Umut Sarac Istanbul

Let's say I could make the same enzymes that other animals/bacteria do in order to digest cellulose. In general, would that cause my body any harm? Let's say I make some transgenic mice that produce cellulase and cellobiose... Is anything going to go wrong? Can those mice eat wood? What about a bug? Is this a surface area issue, which might involve villi? Do you think I could make a transgenic bug that can eat and digest wood? I'm aware some bugs use gut prokaryotes already, but I'm thinking of what would happen if the animal/bug had the enzymes.

I belong to a forum dedicated to BDSM (Bondage- Domination- Submission- SadoMasochism). I am besides a biochemist, which makes me very interested in the transformation from pain to pleasure. There are a thread opened in the forum entitled “From the pain to the pleasure, is there only biochemist?” http://www.clubsumision.com/showthread.php?t=1633 (sorry, it is written in Spanish). On this thread we have argued about the steps who can produce this pleasant sensation when the pain and humiliation are produced in certain situations from certain people to certain other people. For example the action of the endorphins could be stronger in masochist people (perhaps they have…

Would the melting point of DNA be affected by the presence of an organic solvent? My guess is no because there would be no difference between the H bonds formed between, say, ethanol and water. H-bonds contribute little to the stability of DNA of nucleic acid structures anyway... I can't think of any other properties that may affect the melting temperature. thanks.

I tried looking into this, but I could not figure out what this comment was talking about. In some ways it seems self-evident, but I don't know what the discussion is about. It seemed to have received a lot of approval ratings from other youtube members. -

Have recently completed a lot of MAR work. When using labeled Oleic acid as substrate I first checked the pH of sample (pH=8). The pKa for Oleic acid is 5 leading to a 99.9% presence of carboxylate anion in the solution (enviro sample) I guessed that this would instantly lead to the formation of anionic surfactant. When the sample was aerated a foam instantly formed. What I am questioning is whether this would affect uptake of oleic acid. For example the critical micelle concentration for SDS is 8.2mM for Oleic acid it is 50uM. We run MAR at a substrate concentration of 2mM. WHAT would the surfactant created during this reaction do to the cell walls (G-ve) in the sample -…

I like putting a date on certain terms. One that I have recent come across was "membrane blebbing." I get the idea, but I've never really heard the term. I've known of lysis, necrosis, and apoptosis. But then I come across this term called "blebbing." How recent is this term? Anyone get a rough idea? I'm looking at the sources in Wikipedia, and it seems from a quick look that the term came around in the late 1990s.

Is there any proven way to prevent brain damage from alcohol, for example through combination with supplements?

Theoretical could a biological (even synthetic) organism have only the elements Carbon, Nitrogen, hydrogen and oxygen. Could the organism arrange the atoms in such a way so it could take over the role of other elements (sorry if that made little sense) Like Diamonds carbon conducts electricity but because the four bonds on the carbon atoms are taken Diamonds do not. Could replacements for NADH , ATP , DNA be made?

I really just want to make my own phosphate buffers....i use: Sodium phosphate mono and di (156 and 142g/mol respectively) i use the general HH equation (pH = pka + log (salt/acid)) where the salt monobasic and acid is dibasic. Ive worked out the ratio's (i.e for concentration acid + base = 1) and get answers that seem to be corroborated on the net: i.e for ph 7: 7 = 7.2 + log (1-acid/acid) acid = 0.613 salt = 1- 0.613 = 0.387 this works with others answers i.e.: http://www.physicsforums.com/showthread.php?t=163100 (very old post i googled) using this pka (7.21 ~7.2) you should expect 50:50 for a pH at 7.2 right? well all the tabl…

While I am not a scientist, my teenage daughter hold an interest in a career related to biophotonics and I have some related conversations with friends. I recall seeing a webpage flash or pdf, etc. a few months back that described the potential data storage of a single biophoton. I first suspected that I had seen it at Lifescientists.de or Quantumconsciousness.org but can't be sure. If I remember correctly, there was an illustration of a microtubule array and then a note of a potential of either 2 or 4 kb or MB. If someone could point me in the right direction so that I could link some notes to this source, that would be great. Also, an explanation of the implications of …

Anyone? I read about this each year. I keep getting the impression that scientists are stumped.

First part: 1) Used SDS page - size of protein: 4kilodalton 2) Used SDS page again - this time using reagents to break disulphide bonds - still 4kilodalton --> What does this mean? why are the results the same? --> how many peptide strands should i expect given 36 amino acids from the first SDS- page, which assumed 110 daltons/amino acid (4000/110 ~ 36) Second part: protein was analyzed: amino acid sequence as follows: A^2 C^2 D^2 E^4 F G^3 H L M N^2 P^2 Q^2 R^3 S^4 T^3 W, dansylated-R, and dansylated-K does this make sense given results from the first part?? Is it possible for two amino acids to be dansylated (I thought there was only …

Does NADH provide energy to move protons out of the membrane or does it send its own proton out of the membrane? When it oxidizes it produces a H+ so does this have any significance. I mean can any reaction that produce energy move the protons out of the membrane. Thanks!!

Which one would you guys say is more toxic inside a cell and why. No this is not a homework question I was just curious. I would suggest that it is Cu(II) since inside the cell the environment is reducing it would want to react with a lot of different moieties while Cu(I) would be more selective with what it will react. Not coordinate but react.

Does different strength of Murashige and Skoog (MS) media affects the germination and growth of Arabidopsis thaliana? Is full strength MS media not suitable for germination of seeds on sand?

I can not speak good English will be bad if I apologize for my words.How can Chlorophyll absorb light and Chlorophyll can use it?