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About apricimo

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  • Birthday June 13

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  1. I think this is my going out with fires of glory. This forum is filled with a bunch of people who don't have a clue about 90% of topics they post on. This includes YOU. Some of the "answers" you gave to my topics have been completely off base. Why chime in stuff you don't know. Why. People try to answer questions to topics they have no clue about. I'm done with it. Yes and thank you I know how committees are put together but again thank you for knowledge you put in that no one asked about. That seems to be your bread and butter. TO ANYONE WHO READS THIS BE ADVISED THIS FORUM IS
  2. "General science advisor" on his thesis committee? What is general science? First of all what may that be. Secondly if you are on his committee in which case no one would refer to themselves as "general science advisor" why are you asking these things in science forums? Find related articles to confirm or disprove what he said. Were/are you working in the same as lab him and you don't believe what he has to say or just someone who knows this guy through random people? Thesis committee member asking questions about a thesis defense in science forums what a joker. Some people on
  3. Looking to coexpress two vectors pCDFDuet and pETDuet in E. coli. Origins of replication are CDF and F1 respectively. Anyone know if these are compatible? Yes they are compatible
  4. First Part: "Create" no but if you have the DNA sequence you can express it in e. coli or some other organism Second Part: Yes if you have the DNA sequence you can pretty much express any mutant you want. Doesn't mean it will be viable. On the other hand you can create a supermutant that has properties superior to the wild type Third Part: It is done in the lab all the time. Manually? Well if you are the one doing the work then it is manual I guess. No such thing as automatic work that you can do.... Comment: People who work with viruses typically make coat proteins to study. The g
  5. All these types of "theories" start with something that has already evolved. For example, let's say that no theories on how life started exist as of right now, that is, no creationism or evolution or religion to cloud your thoughts. I come into the picture and say that life begins with water because water is needed for everything and continue on with this logic and maybe get two or three more "fundamental" essentials of life. But water is not fundamental, I used water because I see it and I am used to it but who says that water always "was" and whose to say what came from what. Best you can do
  6. You lost me... looking at the character table I don't see a secondary axis.... that's fine my question was more of applying definitions in instances where they "don't apply" but I got it figured out...
  7. Figured it out... In the absence of C2 perpendicular axis it applies to a vertical plane of symmetry... the labels make sense now Where is there i symmetry in C2v?
  8. Ok question about the Mulliken symbols... In a C2v character table z is assigned a mulliken symbol A1. A is defined as a nondegerate and symmetric to principal axis and 1 is defined to mean symmetric with respect to C2 perpendicular to principal axis. Taking Water as an example for C2v symmetry which has no perpendicular C2 axis how do you assign the mulliken symbol A1. Also, same thing for y and x which are assigned B2 and B1 respectively. Don't get how these symbols come about when they don't apply to the definition of what they stand for. Please help....
  9. Has anyone got gabedit 2.3.6 and ORCA 2.8 to work together? If so please let me know how... Gabedit seems to create the input file just fine but when called on ORCA through gabedit it seems to stall...
  10. I am doing some cloning and using Blue/White screening and I was wondering if anyone has ever done this and ran into a problem of having all white colonies. I am using pGEM-T easy system for cloning. My background control is white also, but I don't understand why this would be.
  11. I heard about a "rule of thumb" that you need your vector to be twice the size of the insert. So for a 10 kb fragment I would need a vector that is 20 kb. I can't think of any vectors like this and have you or anyone heard of this rule of thumb?
  12. Hello, I am cloning a large, 10 kb, DNA fragment and I was wondering if anyone has had any experience with cloning large chunks of DNA. Background: The 10 kb DNA fragment has four genes in an operon. I would like to basically take this operon and express the proteins in E. coli so that they can do their thing (i.e. make the product that they make). Anyway, I heard different things from different people as far as what subcloning vectors to use or not use. For example, pGEM-T some people say use some people say that my insert is too large. TOPO vector I understan
  13. define topology with Spartan or with the server website? The energy minimisation in Spartan is on par with any other QM program out there (Gaussian).
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