Biochemistry and Molecular Biology

Hello, I am actually studing to be a lab technician. I have to study the non-invasif prenatal test for Chromosome imbalance. My question is about the fetal fraction. How the fetal fraction is mesured in the case of a female fetus? thank you for your help!

It is commonly accepted that 36 ATP molecules come from one glucose molecule. How is this number arrived at, determined, observed or experimentally verified?

I have been cultivating LP( cells (human peritoneal mesothelial cells) for over a year now, but they never reach confluence. They are newly purchased, mycoplasma-free cells, and I've tried cultivating them on different plastics and coating the plates with fibronectin. I use new EGF in the medium and I even increased its concentration to encourage growth, but the LP9 cell still aren't confluent. How can I solve this problem? Any help would be greatly appreciated!

Hello Assume I have a membrane of volume V, and potential Vm across it.Inside, there's some concentration of sodium Cna(in), chloride Ccl(in) and neutral macromolecule b (Cb). Also, the sodium is being actively transported from the inside to the outside with Jna_a the active current density (constant). The outside has also some concentration of sodium Cna(out) and chloride Ccl(out). And lastly, the conductivity of the sodium is Gna and of chloride Gcl. I am asked the following: Using the parameters above, what is the condition for electric neutrality, osmotic balance and equilibrium for each of the chloride and sodium ions? I answered that following: For electric neu…

I have a mature protein that has been proteolytically cleaved at its N-terminus. I wonder if this degraded protein may subsequently undergo acetylation at its (new) N-terminus? Can a mature protein be acetylated?

When there's no sound, you might tend to notice this high pitch ringing taking over your hearing. But how exactly do you hear anything without any noise? And while we're at it, how exactly can you see splotches when it's pitch black? Do the exothermic reactions from cells really produce enough light for that? Or is it like noise in a bad camera?

Dear all, I have the task to tag my gene with Hellfire (in 3`, so C-tag). I have an idea about what is carrying but I don't find original reference, neither sequence nor complete description. I wonder if someone could know. Thanks for any information about.

What is asynchronous RNA?

I recently tested some synthetic proteoliposomes made via in vitro transcription/translation of a GPCR in a liposome matrix. The manufacturer gives an approximate molar ratio of lipid:GPCR of 150:1, which seems rather high. I'm curious whether anyone has an idea of the molar ratio (or range) you might expect in proteoliposomes made from recombinant GPCR-overexpressing cell lines (either stable or inducible). First post here, thanks for any responses.

Hi can someone please explain to me why triglycirder not occur in the cell membrane? trigylcrider consists of polar and nonpolar part. So why ? thank you so much

For example, a kind of enzyme is characterized by the N-terminal Serine/Threonine kinase. And my question is how to find the kinase activity site, how to make the kinase dead with one site mutation without any reference.

Hey guys, I have a job to design something to increase the chlorophyll inside of plants, not kill them, and fertilize them at the same time. Uh I can get them the fertilizer, but I need something to make them green, which I assumed increasing Chlorophyll levels in a plant would be the best way. Any ideas on how to do this?

Hello guys. I am working on blood of sheep. Blood is hydrolized by pancreas of bovine. I do not know how to check rate of hydrolization. I used electrophoresis but is too limited. I hope you can help me. Thanks for your advice

As per the title. I searched Google and Google scholar briefly, but I was unable to find anything conclusive. I came across some people on another forum who claimed that it absolutely was, but I am not completely convinced.

Hello, I have to come up with a research project for university and I am planning to find possible binding partners of a specific signalling molecule in Drosophila. I have an antibody against the protein so I can co-precipitate the protein and its binding partners. Now my question, can I do the usal MALDI-TOF or would the data too hard to anaylse since there might be many mayn binding partners. Should I purify it before with a 2D gel to get results that I can easier interprete? Thanks in advance Tina

Hi All, Looking for some advice. If I have a protease liquid enzyme of unknown activity how do I calculate activity in U/ml using a colorimteric substrate? All best, Niall

Hi guys, below is a post I made in a gun enthusiasts forum. How likely is it that I can bring home enough lead from an outdoor shooting range to actually poison my child? Does anyone have any expertise in this matter? Bullets have a lead core and use lead styphnate as primer. I'm new to this forum, but figure there must be some pretty savvy and experienced individuals here. Does anyone here have expertise in the toxicology of lead? I'm a new shooter and have a young son (10 month old). I sometimes worry about exposing my little guy to "take home lead" and it negatively impacting his health. I asked my kid's pediatrician about this and she seemed surprised that I…

How to make homogenization from small intestine

Hello guys. I am working on blood of sheep. Blood is hydrolized by pancreas of bovine. I do not know how to check rate of hydrolization. I used electrophoresis but is too limited. I hope you can help me. Thanks for your advice

Hello. What kind of method fits to work on blood

It's pretty much the same concept...is it just that microarray's are high-through put and ELISA's are just less sample abundant? I don't see how a microarray could be more efficient in the theoretical sense (bind a protein to a surface, and react with antibody, conjugated anti-body, and measure signal). Perhaps in the volume sense of the number of samples, but I don't really see anything else. Am I missing something here? ~EE

Hello I want details regarding RNA-RNA EMSA. I require your valuable suggestion for my problem with experiment. I used two RNA for gel shift assay one of which is labelled with Digoxygenin. I get some shift in EMSA. I also perform the same thing with unlabelled RNA and perform EMSA assay as previous one and stained my gel with Ethidium Bromide but not able to detect any shift. I am not able to conclude anything out of it. Kindly suggest regarding Waiting for your reply Regards, Hasmatbanu Buchad

Hello everyone, I've made a recombinant protein with a long (GGGGS)6 linker between two domains, and refolded it from inclusion bodies by chemical refolding in the presence of a redox couple. Under non-reducing SDS-PAGE, I get a fairly broad (maybe 5-10kDa range) coomassie-staining band. This band is much more compact under reducing conditions (and shifted upwards, which I would expect). My concern is whether the apparent divergence in mass of the band is indicative of incomplete oxidative refolding, or whether this is a property of having a long flexible linker between the domains. Does anyone here have experience of expressing proteins with long flexibl…

Took a tour at a nearby distillery and they were fermenting the mash on huge tanks with no lid and at 66 degrees Farenheit. It seemed unusual to me, without much knowledge of producing ethanol. Does 'no lid' and '66 F' seem normal to you ? Or it all depends on the yeast type, and is there a yeast that 'works' at that temperature ?

Hey everyone, my question is, what makes the different in PKa of alpha amino and epsilon amino in amino acids? My teacher wrote to us that alpha amino is near the carboxyl group, and the O is electronegative so it want to gain an electron, but I don't get how this thing makes the amino group near the alpha carbon to want to lose a proton? Thanks