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Thanks for your question. Just as this is your first gel electrophoresis of PCR, this is my first time reflecting on PCR in quite a few years, so don't take my word for it completely but I'm going based on memory here to try to help you/answer your question since I see no one else has yet. Hopefully someone else chimes in and corrects me where I'm wrong. 1.- The impression I'm getting from your professor, since she's asking you to do them separately, is that she wants you to retain how to make the mix and also she wants you to be careful/not wasteful of the products. It's a tedious process, and the best way to learn is by repetition. The fact that your professor's asking you to repetitively go through the protocol shows me she really wants you to learn and perfect your method of PCR. More importantly, I think your professor doesn't want you to affect the quality of the outcome of PCR. Perhaps making a larger batch could cause you to dilute the primers more than what they should be, which with one mistake the whole batch would be ruined. From what I recall (years ago), primers are expensive so she also probably doesn't want you to waste the whole thing in one go. Instead, if you work on them one at a time, it'll help for keeping the outcome consistent with minimal/negligent errors. So, although I don't think it's a necessary measure, I do feel it would be the smarter thing to do if you really want to ingrain PCR technique and also take precautionary measures as well as keep the outcome consistent. Also, it could be the case that one of the primers may require a different step which cannot be applied to other primers. 2.- Primer = DNA piece that will be basically copied/recreated. DNA is molecular compound which means having the DNA of one species of bell pepper should be similar to another species of bell pepper. This means the environment in which DNA reproduces should be the same. So, no I don't believe each primer will have temperatures different than one another's but perhaps there may be additional steps required to produce a certain result you're looking for (one of the species). 3.- Blank is supposed to stain so that you have an independent/standard component to compare the rest of your strands with. The ones that didn't show either weren't dyed or something may have gone wrong – most probably a forgotten ingredient (which is probably the reason why your professor wants you to repetitively make the batches), or something may have gone wrong. This link may help with that: https://www.fws.gov/aah/PDF/PCRTS1_NoBand.pdf Try troubleshooting using that. Hope this helps!

Chess. Lol. Wanted something short and sweet to login and play chess online with people. Yup.

College is where you go for undergraduate studies in a certain concentration of a subject (examples of studies that you would major in, in a college are: anthropology, biology, chemistry, math, physics, english, linguistics...just google any college/university and search through “undergraduate programs”). Graduate programs are an even more specific course of studies that you undergo after completing a bachelors degree at a college/university. MCAT is an exam taken for medical schools. GRE is an exam taken for graduate schools. Are you looking to find a way into medical school or graduate school?

Definitely agree with you and thanks so much for the additional post!!! Really appreciate you taking the time you do to answer all of it!

If longer exposure of heat leads to breakdown, does it lead to breakdown of that chemical to make it safer to drink or no?

Exactly! I just don’t understand how the thought of arming teachers was seriously considered—even if it was momentarily so—as a reasonable solution to school shootings... How? I just don’t get it.

I just caught this, too!—which is why I tend to read posts a good few times before responding. Big oops!

If they arm teachers, we’re going to hear about it on the news one day of this teacher who was “mentally ill” and attacked children. It’s a horrible idea. And I hate to emphasize this but it’s starting to seem more and more like a govt run conspiracy to dismantle the public. If you don’t believe me, just check Trump. What rules hasn’t he broken? And yet he’s still in office. He should have long since been impeached, but he’s not. And I hope for goodness’ sake there’s no one out there thinking Trump has anyone’s best interest at heart except his own. He’d run to take up the opportunity of dismantling the people and becoming a tyrant. They want YOU to choose to let go of your own arms. And to be honest for a long time I supported gun control, but the conniving ways of Trump and the government have proved that this issue could be an insider thing. I don’t know what the question was, I got too passionate about this. It’s just downright upsetting!!!

Thank you! Thank you!! Thank you!!! This made my day!

Thanks! Yeah I mean without having that little bit of insight I’d be incredibly bull-headed for no reason. I resent that quality—would hate to be unreasonably stubborn. I’ll definitely look it up. Yeah, after reading CharonY’s first post about twenty times it made more sense to me that that was most probably it. Kind of ruined that magic I felt about the bat. Sigh. I see... Thanks for this post! I’m a little confused again though so I’ll definitely read your post a few more times and compare it to previous ones... First, it could have been the light in my room, emitting heat that could have interrupted the way my camera processed a photograph... But then, it could have been the bat’s effect on the lamplight and ultimately the way the photograph was captured... Yet, the bat had little to nothing to do with it, and so it had to be the camera itself with the way it takes photos... But it could also be— so much regret for not taking more photos. I’ve yet to look back on them and find them. From this, I got shutter speed... Thanks again CharonY, I’ll definitely try this. Exif data? I’ll be in touch

In-laws not getting along is their problem. That has nothing to do with the couple’s personal life. “Siblings are much more likely to be matched than parents but only about 30 per cent of people needing a transplant will have a compatibly matched sibling.“ What about the other 70%? From strangers not even living in the same country (see article). Seems as if, regardless of whether or not one marries outside their gene pool, transplants are simply hard matches to come by as they are—even amongst family members...so it’s not that great of a reason to avoid an interracial relationship. Now we’re getting into the semantics of this notion of “belonging”. You belong when you are acknowledged, appreciated and loved. Do you have any pets, maybe a dog? They don’t communicate in the same language as you, let alone participate in the same cultural customs and traditions as you, but I bet you you’ll definitely feel a sense of “belonging” when you’re with your pet... How sad is that? The people you surround yourself with (certain family members, I assume) won’t allow you or your of-a-different-race spouse or interracial children to feel a sense of belonging just because they “can’t communicate in the same language”. Try hugs? Kisses? ...The idea that a dog can love better than most humans is just sad. As for your last point, I’ll speak from experience: I’m a “mixed race, third culture” person who grew up not feeling lonely and in fact have a beautiful interracial relationship with a Hungarian man who understands me completely. It’s a soulful union, and as different as he and I may often be, we complete each other. I will add, I’ve never spent much time with neither my paternal grandparents nor my father’s side of the family, but it hasn’t impacted my life even in the slightest. When love happens between two people, it happens. Let it happen. Interracial, opposite religions, whatever it may be, when it happens, let it happen. It’s a kind of experience that can’t be measured by your “weighing of pros and cons”...

I just saw this, thank you. It seems though as if everyone’s set on the fact that it’s the camera...but that didn’t happen when I was further away from the bat. Only when I was near... That’s why I held the thought in my mind like a conviction that the bat must be emitting something that was causing my camera to take unclear photos... I’ll try anything at this point though. Thanks for the suggestions

I just had mine, black, with no regrets—and I plan on having at least four more by the end of the night. Any thoughts on this? Is there any molecular biologist, geneticist, chemist, or whoever, who wants to chime in on that news article and explain why or why not the California judge’s ruling is harsher than it should be? Was it reasonable, or does it seem that there’s a motive behind passing the law that was passed (Proposition 65)? The dr in the article says odds aren’t likely that someone would develop cancer from drinking coffee (I guess because of this negative feedback effect—is it?—that coffee has on the liver as it detoxifies the liver after it’s worked long and hard to clean up the body’s toxins, protecting the liver from cancer despite potentially being carcinogenic itself)...but do the consequences of consuming large amounts of coffee depend on a specific diet—aka the “average american diet” that goes something like 3-5 meals a day, mostly meat, bad cholesterol, carbs, hardly ever much fiber, etc., or is it worse when following a vegetarian lifestyle? Is that possibly why the law was passed? If the law was passed for coffee, why not for other foods, as the article mentions (example: french fries)? Specifically, isn’t it the case that not only heated foods but also some nonheated foods have cancerous effects on the body? Your thoughts?

Made me laugh so hard. U r an astrological genius #Level10