Genetics

If governments would widely implement positive eugenics, could we create such world: all people around us are highly moral, intelligent, polite, altruistic and prone to help each other?

I understand the idea of how anergy happens but I cannot find a clear explanation for why it happens and why there is no co-stimulation? What regulatory mechanism is preventing the B7 and CD28 receptors from interacting? I ask because at least in the thymus, the T cells are in an isolated and controlled environment and they have a hard time leaving if they have receptors against self. However, the peripheral tissues are like an open area. So what is the regulation that is occurring there?

How can we slow down mitosis and meiosis or one of the two especially mitosis and what can be brought about?

If one always has one allele from each parent, how it's possible that in certain areas of phenotype only features of one parent are expressed? For example, it's possible for daughter to look exactly like her mother or have personality after her etc.

Forget about transhumanism. Idea... Each human being holds their own immortalised DNA within their reproductive system.. Sperm cells, Embreonic cells all house Immortalised DNA. All you need to do is break down those cells, either find a cell that has the immortal DNA and allow it to multiply or use all the cells. Here is how it works.. You aged DNA will recognise the Immortalised DNA within the cells seeing as the immortalised cells are produced in your own body, and they will prefer to have the Immortalised DNA over the aged DNA. When you inject or consume the Immortalised DNA, it will gradually take over the old DNA. Eventually you will start to age in re…

Hello, for my Masters thesis I'm doing a Footprinting experiment with HeLa cells. For this the DNA is cut at unprotected sites where no proteins are bound. Via 2 PCR steps the fragments are amplified and then analyzed by capillary gel electrophoresis. Most protocols use Vent polymerase for the whole procedure because it creates blunt ends which is important in the first PCR step of the procedure. However I wasn't able to get good results with Vent, so instead we used iProof to create blunt ended products, and iTaq for the 2nd amplification step because of its higher effectivity. My question now would be if iTaq adds an A at the end of the fragments like the ordin…

I'm looking for a little help or just confirmation on whether or not the results I'm getting from BLAST mean anything or if I am completely not usiing this tool properly. I am using a sponge sequence Geodia neptuni 18s ribosomal RNA gene, complete sequence GI = 53771873 and running a nucleotide blast against all bacteria and get this result; 92TUW20U01R A score on the alignment of 1459 and 82% identity with the bacterial sequence identified as Azotobacter vinelandii is far better of an alignment than blasting that bacteria against all other bacteria. That doesn't seem right to me.

What causes favorable mutations in the human genome ?

Hello, As we all know , AIDS is an dangerous disease. AIDS (HIV) virus attacks a CD4 cells and makes it's own copy by sending his RNA inside the cell. Using reverse transcriptase, viral DNA is formed and lytic cycle continues. Here is a thought, We know Genome of HIV virus, right ? What would happen if can encorporate a gene within CD4 cells growing on agar, which produces REN ( Restriction endonuclease) that cuts the Viral RNA into small pieces,thus making it ineffective? We can tranfuse the GM CD4 cells into an affected person. As there are no new infections possible, number of viruses will decrease gradualy. We might be able to cure AIDS ! I am …

If mother and father do not have flat feet, can the offspring have flat feet? I believe pes planus is a dominant trait, but admittedly, when it comes to genetics, I am lacking. Can someone clarify this for me. Thanks in advance!

Im not sure if someone already had a thread about this...But my friend told me he saw somebody with two different colored eyes and now he wants them:confused: I told him about these forums and he wanted me to ask, is it possible to give yourself two different colored eyes without hurting yourself or blinding yourself?? p.s w/o contacts of course...thanks

Hello, Im reading a book called:Textbook of Personalized Medicine As part of the book defines SNP but its written a few obstacles for their use which I do not understand well. Do you know why these 4 items are considered as the obstacles for the use of it? I put the part of the book related to SNP: "Using SNPs or a small set of SNPs is considered to be an excellent tool to discover genes for psychiatric disorders and potentially an excellent tool for psychopharmacogenetics as well. There are, however, a few obstacles for their use: (1) high-throughput, low-cost genotyping assay systems; (2) definitions of good disease phenotype; (3) a good collaboration effort amo…

I have been sick for many many years, i have had a medical investigation going on for 9 years and pretty much all my tests has been normal, at least nothing that can explain my symptoms. I strongly believe that i was born with a disease and then my symptoms became fully developed around the age of 13 or 14. its either that or i got my disease / infection when i was very young and it has stayed in my body ever since and cant be found, that just seems very unlikely to me. 10 years ago i got cured for 6 months when i took Roaccutan (Isotretinoin) against accne. I took Roaccutan for 7 months, a couple of months after i stopped taking it all my symptoms went away and then gra…

Soooo what exactly causes increased vigor in some F1 hybrids? I am talking about plants. Is it domianance or overdominance? Even when I read explanations about these two hypothesis,it is not explained in detal,on a genetic and molecular level.Also,what I don't quite understand,when creating vigorous F1 hybrids,do the pure lines *need* to be homozygous to achieve the increased vigor effect,and if so,why? Or is it done to simply impove uniformity? Any answers and/or sources of latest research on the topic would be greatly appreciated!

Hi, Im researching to find a list of miRNAs in melanoma CSCs but unfortunately I havent been able to find an article which exclusively related to melanoma stem cell . Do you know any paper about this topic?

Hiw many genes have so far been identified as contributing to development of depression and anxiety disorders?

Quite recently Svante Pääbo director of the Department of Evolutionary Genetics at the Max Planck Institute said “It is still unclear exactly how much of the complete Neanderthal genome exists today in people, but it seems to approach something like 40 percent.” But 10-15 years ago the hypothesis of neanderthals being involved in making modern people was considered by most of the scholars as ridiculous. They believed that time all modern humans to descend from one person "chromosomal adam" - a pure sapiens. I've just found this - where is that beautiful man on this tree I wonder?

Hello everyone! I have one question that I found a bit difficult, could you help me? Here it is- We're crossing white-eye female of D. melanogaster and a wild type male and the offspring is white-eye females and red-eye males. The question is how to explain the result. I was wondering if it can be because of different then 1( X:A) ratio. For example, if the ratio is 1.5 we have a Metafemale with 3 chromosomes X. Is there any possibility that on this additional chromosome we have red eyes gene allel which is then forming a red-eye males? I hope I wrote everything clearly

Hi I have recently joined the largest online cytogenetics discussion forum hosted called the Cytogenetics-Methods-and Trouble-shooting Forum. The forum is hosted by Google and its free to join. Thier web link is : http://groups.google.com.au/group/cytogenetics-methods-and-trouble-shooting. The group covers all areas in cytogenetics including: discussions of blood/bone marrow/solid tumour/prenatal/skins/POC cytogenetic methodologies. Trouble-shooting all cytogenetic problems such as harvesting problems, banding and cell culture. I highly recommended it to others to join.

Hello everyone, I have Myopia or near-sightedness. My mother is far-sighted and cannot see stuff that's near her without glasses. Is it possible that I still got my Myopia through genetics?

How did only the offspring of mitochondrial Eve survive and the offspring of other contemporary women died until today?

As you are probably aware mice and hamsters come in a myriad of colors. But I wonder exactly what the different alleals are doing that causes the color change. For example I've been experimenting with breeding roborovski hamsters. They have developed a few mutations in captivity. Husky is a beige color, dominant spot which is some white ticking around the rump and ears, and recessive spot which agouti color fur only in patches emanating from around the spinal area. The odd thing is when you mix these colors you get unexpected results. For instance 2 dom spot and 2 rec spot allele results in an animal that is completely white. 1 dom spot and 2 rec spot alleles res…

Hello Everyone! I am wondering about everyone's position on genetic testing? Do you guys agree with it or are you totally against the idea? I wrote a paper in my college English class about genetic testing and posted it on a blog the rules prohibit me from advertising, so I'll post more about it here in this thread. I feel that it is a great method to prevent or start treatment early on diseases, and a way for scientists to learn about what genes cause certain mutations.

As per the title, I'm looking at doing some gene expression analysis in S. cerevisiae as well as some pathogenic fungi (C. neoformans, for example). I am fairly okay with extracting the total RNA and assessing integrity of the samples, having done this pretty extensively in my last job, however I am unsure of where I should go for isolating the mRNA. My plan currently is to use the Qiagen RNeasy kit followed by mRNA purification with their poly A minikit (I think it's called Oligotex). It is a little expensive, however. If there are kits that are better or the same in quality for less, I'd be more than willing to try them. Biology is an expensive discipline to be involved…

hey... i have performed a small scale siRNA screen , comprising of 100 genes. i was wondering, if a data normalization approach like standard score or feature scaling can be applied or recommended, considering the small screening size? Thanks