Homework Help

How many fragments will be generated if a 6000 bp plasmid construct was cut 2 times with the restriction enzyme SmaI at 1000 bp and at 4000 bp? What will be the size of these fragments? How many bands will be visualized on a gel?

An 8000 bp plasmid construct has 2 restriction sites for EcoRI at 0 bp and at 5000 bp as well as a restriction site for HaeIII at 1000 bp. If the plasmid was digested with HaeIII, how many fragments would be generated, and what are their sizes? if it was cut at 1000bp by HaeIII then wouldn't it be just one fragment? but i am confused as to why they would also have EcoRI in there? so the answer to this question would 1 fragment with a size 6000bp? right?

An 8000 bp plasmid construct has 2 restriction sites for EcoRI at 0 bp and at 5000 bp as well as a restriction site for HaeIII at 1000 bp. If the plasmid was digested with HaeIII, how many fragments would be generated, and what are their sizes?

Can somebody please tell me in what I should do in this question and how to work it out and also explain what the difference is when it says "excess" or not being excess. thanks

Hi, I really need help answering the following, thanks: When an action potential reaches the end of the A, the message it carries is converted from a/an B signal to a/an C signal in order for the message to be D to the next E. The region called the A is made up of the B terminal, the C membrane region, and the small space between them known as theD. The E is an extremely small gap, about F nm. The G membrane contains specialised H to which the correct I substance can attach. The membrane voltage during undershoot is: A (select a logical membrane voltage value from this list: +60 mV; +50mV; +40m V; +30mV; +20nV; +10mV; 0mV; -10mV; -…

Hey, so i'm having a bit of a hard time trying to work out these two questions. If anyone can provide any insight it would be much appreciated. 1) Convert the 7-bit binary code 011 1101 into a suitable Hamming code that can detect and correct a single bit error. Use EVEN parity, writing your final answer as a 3-digit hexadecimal. 2) When using Hamming code with EVEN parity for 7-bit ASCII characters, the following symbol is retrieved: 01100110101 Assuming a 1-bit error, what was the original stored symbol? Write down your answer as a 7-bit binary, with no spaces.

So my professor sucks. I feel bad about saying it but I´m like almost to the end of my course and I still understand nothing of Stoichiometry. I have a lab of just Stoich questions and I am really struggling. She gave the answers but I do not want the answers, I want to know how to do them. Anyway at the moment here is the question I am stuck on (before going on to the next ones) I´ve already balanced the equation. Al(OH)3 + 3HCL --- AlCl3 + 3H2O c. What mass of water (H2O) is produced by the reaction of 10 grams of AL(OH)3? All my attempts at anything have been wrong. I´ve done random stuff like 10g AL(OH)3 = .128 moles AL(OH)3 H2O = 18.…

Please excuse [deflection] error. Could you help me with the following. Using continuous beam theory, constructing BM diagram from points b to c, to calculate the max deflection. I only found a have a single solution, though the BM digram show two points of zero bending. I can provide the solution. your thoughts. [edit: Rb = 685 N] Please find revised attachment. attach1(revised).pdf

Determine scalar, vector and parametric equations for the plane that passes through the points A(1,-2,0), B(1,-2,2), and C(0,3,2). This is what I got... AB AB=OA-OB =(1,-2,2)-(1,-2,0) =(0,0,2) AC=OA-OC =(0,3,2)-(1,-2,0) =(-1,5,2) AB x AC (0)(2)-(5)(2) =0-10 =-10 (2)(-1)-(2)(0) =-2-0 =-2 (0)(5)-(-1)(0) =0 The normal vector is (-10,-2,0). -10x-2y+0z+d=0 -10(1)-2(-2)+0(0)+d=0 -10+4+d=0 -6+d=0 d=6 The scalar equation is -10x-2y+6=0 The vector equation is (1,-2,0) + s(0,0,2) + t(-1,5,2) Parametric Equations: x=1-t y=-2+5t z=2s+2t Did I do this right? Thanks for the help in advance.

So i was given these questions to do by my teacher and for 27, not only was she unable to help with the question but even didnt know about shapes of molecules. Can somebody pls teach how I can identify shapes from just looking at an equation plss. Also, my teacher couldnt even do 17.... shes the one who gave out the homework... i really need help cheers

Hello, I am here to ask you some questions about anatomy. Which answer is correct? a)the fibers directed to the colliculi through brachia conjunctiva superiores cause the activation of the medial longitudinal fasciculus; b)there are fibers that depart from the pretectal area of the mesencephalon and are directed to the neurons of Lamina VI in the spinal cord; c)mydriasis is linked to the action of the ciliospinal center d)a+b are true e)a+c are true Which of these nerves does not pass in the cavernous sinus? a)III b)IV c)VI d)VIII e)VII Thanks a lot!!

Can Somebody pls show or explain how the dot and cross diagram works in this question. I've tried drawing in many different ways but this ones a little weird. cheers

Hi, The problem uses a continuous beam, but I'm unsure if I'm correct in solving for the max deflection between pts A and B (I've included the BM and SF diagrams btw pts A snd B, only). Deflection should be a maximum when SF = 0. I've also formulated an equation to solve for max deflection using the second area-moment, where 'x' is measured as the distance from A. The BM at B ([latex] M_{B} [/latex]) is straight forward, but the BM due to load is non-linear, I believe this needs revision, but your thoughts. [latex] \Sigma A\bar{x} = \int_{0}^{X}wx\frac{x}{2} \,dx - M_{B}\frac{x}{L}\frac{x}{2} [/latex] BM cont beam.pdf

Sorry, I'm new in this community, Scienceforums.net looks interesting so I JOINED ! How to solve this !? I'm not looking for an answer I just want to know how to solve it, formulas or etc? what do I need to do, sorry if my English is bad I'm still an ESLC Student ! in 11th Grade ! correct me if I spell something wrong ! Thanks xD By the way, I may be in the wrong site ! Because this is Science ! not Geometry ! I apologize If I cannot post Homework about other subjects !

https://books.google.com/books?id=Y8nl4GFx1uIC&pg=PA5&lpg=PA5&dq=a+reduced+mathematical+model+of+the+acute&source=bl&ots=cCcTGLBb49&sig=NJRP0-rd49BJUcGVAAt1SJoTHIw&hl=en&sa=X&ei=4ZoRVerGMsHjoASq8IGoCA&ved=0CFcQ6AEwCA#v=onepage&q=a%20reduced%20mathematical%20model%20of%20the%20acute&f=false The above is the book. The picture is Figure 11 on page 27., which I try to reproduce. The ODE is (2.9)-(2.11) on page 24. The vertical coordinate is marked P0. But in order to solve the ODE systems, I have to set the initial value of P(t), especially I have to use numerical method to solve it . Usually, we set P[0]. But P…

Hi all i was wondering if any one could help with this question. i can do the calculations just need to see the formula and how it is set out the material i was sent to help me has nothing to do with this type of question. In a closed system 0.3kg of gas at 373 k is expanded isothermally and reversibly from 1MPA pressure to 200 KPA. given that Cv = 718 J/kg K and R = 287 J/kg K determine: the work done. the state of energy transfer within this isothermal process explaining your answer. many thanks scott

Answered The Following Questions Plz 01. The Combination of Py and Pz Orbitals can not form bonding & antibonding molecular orbitals. 02. Mn3+ion µs is 3.26 will it be covalent or ionic complex? 03. VBT does not explain spectral and magnetic properties of metal complexes 04. Non-axial orbitals are triply definerate 05. In s.pl geometry the ∆ sp between dx2 – y2 and dxy is equal to 10 Dq 06. In TD geometry the ∆ t is equal 0.45 ∆0 or 4.5 Dq 07. In tetragonal geometry dx2 dy2 is lesser in energy than dz2 08. The isotropic filed spilits into eg & t2g orbtals in oh geometray 09. In TBP geometary the isotropic field spilits a,g, eq & eg 10. ∆ o For in […

Please refer to the attached file. Can any one please explain to me where the (x-0.01) for O2 comes from? I have been stuck here for a long time. Thanks.

are both conc. and dil. sulphuric acid strong acid and hygroscopic ? if conc sulphuric acid is hygroscopic that can draw water from the air , can its H+ ions become more ionized and result in lower pH? i would be very grateful if anyone can answer me

Hi I have done lots of research but I still cannot figure out if there is a difference between inflammatory responses to viruses and bacteria? immune response to viral infection is composed of monuclear cells whereas bacteria trigger cytokine and chemokine release through macrophages but then there are also mononuclear cells ? so is there a difference in the inflammatory responses to bacteria and viruses as I am thinking ? or are they same. any immunologists help me out please thank you in advance

So I have a "hypothetical" worksheet for biochemistry. I have to separate the following 8 proteins as much as possible: Bovine Serum Albumin Myoglobin Hemoglobin Cytochrome C Alpha-lactalbumin Ribonuclease Ferritin Catalase I started with Size-exclusion and got these for the light proteins: Cytochrome C M`yoglobin Alpha-lactalbumin Ribonuclease And heavy proteins: Hemoglobin BSA Ferritin Catalase I figured out ways to separate the light proteins further and essentially have them completely separated. What are some techniques I can use to separate the heavy proteins?

So I have calculated everything and everytime i get an answer I always seem to end up having more Riboflavin in the power of the pill (which weighs less at .9647g) than the full non-crushed version of the pill (.9695g). I have been using the equation of (c (mg/L) = mass (mg)/V (L)) and I am plugging in the C value that I obtained which is about 51 ppm and then the two mass values and the volume of the dilution which is 250mL. Any help on seeing what I am doing wrong or what the variable letters mean as I am also confused on that front will be helpful. Thank you.

has anyone done the lab with an iron nail, filter papeer and copper sulfate, adding the NaCl and water? I was wondering what is the NaCl doing in the lab? This is urgent, thank you

Does anyone know few clinical importance of cystic fibrosis?

What will happen when i put 7g of CuSO4 then filter paper, 7g of NaCl, then filter paper, sanded iron nail, and H20. I am really stuck and i don't know how to explain it scientifically of what will happen, and how? And what is the green color in the experiment, and where the gas bubbles come from, what reaction? Thank you so much