Genetics

Amplifying means making many the same copies (replicates) of DNA sequence. This can be made by different methods including cell cloning. One particular method of DNA amplification has proved important in recombinant DNA technology and is used in a series of applications in medicine and forensic science. That method is PCR... Please share your ideas about: What is PCR?

The researchers who investigated bioluminescence and quorum sensing found that E. coli transformed with a plasmid containing a 9 kb fragment of V. fischeri DNA could glow when the cell population was dense. They mutagenized these E. coli cells and isolated many mutantions that map to the 9 kb fragment and prevented the cells from glowing. They then performed complementation testing with these mutants by transforming E. coli cells simultaneously with two plasmids, each containing the 9 kb fragment with one of these mutations. To ensure the E coli cells were transformed with both plasmids, one of the two plasmids had a gene conferring resistance to ampicillin, while the oth…

Hi all, Im trying to do a FISH on DNA combing for pericentromeric regions. As you may know, the human centromere is actually separated into two distinct regions: 1) centromeric region - which is involved in kinetochore binding 2) pericentromere region - a heterochromatic region which is involved in sister chromatid binding. Im looking for pericentromere specific probes which do not overlap with any other region (centromeric or non centromeric). Does anyone have a clue whether this kind of probes/BAC/HAC/anything else exist? if so - a link would be great! Thank you! Matan

How many genes are involved, solely or mostly, in the functioning of human brain?

Hi I am wondering if it would be possible to put the autoflowering cannabis genes into food crops and even redwood trees to plant in Alaska. The gene gives the cannabis plant the ability to flower under 24 hours of light and that might be a good thing if you are a tree or plant in the north. The permafrost is melting out in many areas anyways so maybe it can support large fast growing cold tolerant trees for cleaning air and harvesting wood. Also since fusion power is hopefully right around the corner maybe the trait would be beneficial for space travel and feeding the worlds population. What you think?

I'm interested in performing a mitochondrial DNA test on a bone sample, but am having trouble finding labs that actually perform this type of test. I want to perform a test on the sample in order to see whether or not the sample belongs to my mother. So, technically, this would be a paternity test. If you perform an Internet search on paternity testing, you'll get a ton of hits that give facilities that will perform swab DNA tests. These are the types of tests that most people use in order to identify a baby's father. We have no shortage of those facilities. But when it comes to mtDNA testing on a bone sample, the closest one I could find is on the other side of…

Im taking a comparative genomics class and working on an assignment where we have to find orthologs that are expressed in thaliana, which may give some insight into ESTs that are regulated in olives when infected by a fungus, specifically OLEST23. Genetics isn't my strong suit, but im required to do at least one module per year. If anyone is familiar with this database can you give me some insight how I might go about finding the ortholog?

Hi all, For an upcoming third year uni genetics exam in a past paper I was asked to derive the Hardy-Weinberg equation for two alleles in the population. Whilst I can use the equation no problem I've never been asked to derive it before.. If someone could walk me through some simple (as possible) steps that would be very much appreciated! Thanks all! A stressed biochemist.

Hello, I'm new and I have this problem that I cannot figure out by myself so I thought someone could help me here. When we know how many recessive homozygotes we have, then we change it into decimals and a square root would be the frequency of q (the recessive allele). It is simple and logical. Why do I sometimes see that people calculate q as the suare root plus 1/2 pq? I saw it in Campbell and the example was about codominance. So are the calculations different when you have codominance and three phenotypes for three genotypes? Thanks. : )

Ok, I am very sorry, but I am very new to genetics, and know relatively little, so please bear with me! Talking about dog genetics, many breeds have different types of coats A poodle will almost always have the poodle coat, since purebred poodles have been bred with other purebred poodles for many many generations, I would assume all the traits associated with the poodle, such as the shedding and coat, are all with 2 pairs of the alleles that cause these traits. This would be the same with other breeds that are crossed with the poodle, such as labradors, in that they will have a double of the gene which causes their coat due to only being bred with other memb…

newest research confirms that fair skinned people have lowest vitamin d levels and thus suffer from lowest bone density, osteoporosis, rickets, multiple sclerosis and other health problems caused by lack of vitamin d. this scientific findings go against the mainstream theory of nina jablonski who says fair skin evolved for better vitamin d absorbtion in low uv environments. nature would have never selected a mutation which hinders you to absorb necessary levels of vitamin d by threatening you with skin cancer. the fact that fair skinned people need sunscreen and vitamin d pills to survive is evidence for suffering from geneic disorder rather than being adapted to cloudy n…

http://gizmodo.com/frozen-tardigrade-brought-back-to-life-after-30-years-1753152359 Sorry if this is the wrong place for this post am only posting to get a few good responses if possible . Is there any way we could extract the properties in the gnome of the tardigrade and genetically engineer them inside of our own gnome ? Nanotechnology could be instructed to implement the genes ?. If we could we could probably stay alive through space travel. If anybody is doing there A-Levels don't listen to what I am saying.

Hi everyone, new to the forum - wondering if anyone here has any experience with gene trapping, and may be able to help me interpret the 'details' section on MGI (mouse genome informatics). Specifically, I'd like to know which genes have been disrupted. (I've attached a screenshot of the targeted allele details section for one of the gene traps I want to look at) The method is new to me, and no one in my lab has any experience with it - I'm also still learning my way around all the functions of MGI. Hoping one of you might be able to help me out!

Hello, I am facing a situation with a pregnancy with single female fetus where amniotic karyotype revealed a 45XX result in one culture and 46XY in the other. The father is 46XY, the mother is 46XX. There was no comment on the result but an indication to repeat the amniocentesis - this is not an ordinary procedure to indicate easily, especially when it comes to repeat it. To me, this result really makes no sense, the discrepancy between the two cultures seems to indicate different origins. What is your opinion on this result? Thank you in advance!

Is cross species genetics possible, like splicing animal dna into human dna?

I have a genome sequence for a bacterial strain with an ORF that I'm sure encodes for a luxR gene (or similar), so a transcriptional regulator. I've also found the start and stop codon, but am having trouble finding the ribosome binding site (i.e. the Shine-Dalgarno sequence). I know typically it's AGGAGG, and that it does not always have to be so, but I'm unsure how 'different' it can be from the general consensus. Either way, I cannot seem to find a RBS. The closest I've managed to find is GAGAG, but I'm still uncertain about whether it could even be the RBS and am now starting to wonder if the ORF I have found even encodes for a real gene. I also know that a RBS is on…

I am trying to find what the percentage or even general odds of a baby being born with a cleft chin, whose parents don't have cleft chins, and whose grandparents didn't have cleft chins. From what I've read, virtually any mutation is possible with or without the genetic history. So, I'm not asking if it's possible, but apart from terms like "rare" or "uncommon", is there any data or calculation that can suggest just how rare it is? From what I've perused, it is suggested that it is more common for a mother or father with a cleft chin to birth a child without one than the opposite. Any thoughts on any of this?

Hello, my name is Benjamin Stroup and I will be performing a student study on Heredity. I will be studying a simple way to predict descendants traits. For this I will need your help in research. I would like if as many people as possible could fill out the form below on you, your parents, your siblings, and your grandparents. Fill in as much as possible. Thanks in advance. Sincerely, Benjamin Stroup FILL OUT THE FORM BELOW FOR YOU, YOUR PARENTS, SIBLINGS, AND GRANDPARENTS Eye Color (See attached image): Hair Color: Hair Style (Curly, Straight, etc.): Nose (Pointed, Round, etc.): Earlobes (Attached, Detached): Height: Weight: Age: Facial Hair (Male)…

I have been trying to research this for a short story I am writing but to no avail. What karyotype, SRY mutation, or other genetic mutation would be required to produce a true hermaphroditic human that has both fully functional sexual organs?

I knpw that 23andme has done a Gwas of sexual orientation and they couldn't find a genetic association for male or female homosexuality. I guess individual prenetal hormonal and epigenetic processes are the major reason why some people are gay. But I have still the opinion that there is indeed a unknown genetic reason why some people are gay. Can we say when a gwas was unsucessfull in finding a genetic association for a phenotype there isn't a genetic background existing? They also have done two linkage analysis for male homosexuality. If the major cause for homosexuality is prental hormonal/epigenetic and male homosexuality is very common in the population, s…

Hello i have a question about the expression of miRNAa in cancer tissues I want to know does it always mean promoting invasion and metastasis when a miRNA upregulates in cancers?

Do you believe there was a genetic predisposition that caused Freddy Mercury to be homosexual?

So yesterday a question popped up in my mind: Let's say we know all the genes responsible for our DNA repair system and let's set the ethical aspect of embryonic genome editing aside, do you guys think, that the mutation rates would go significantly down if we were to insert in one generation a copy of each gene into our genome?

Does any thing in this life affect the genetical structure permanently? I heard that genetics evolve slowly but could there be a sudden genetic mutation in the human body , if yes is it or not impossible to correct ? Feel free to share any information that might -even if slightly-help Thanks , regards.

Hy everyone! I am 24,5 years old and I am from Belgium. I have a question about delayed body growth. I have noticed something peculiar. I measured my body height a few times over the past years. At the age of 16 my height was 1m63. Between the age of 18 and the age of 23, my height was 1m74 to 1m74,5. Today I measured my height again and it was 1m75,4. It must have increased by 1cm over the past year/year and a half. How is this possible and could I grow even more? I always measure it in the morning, because I am aware of the fact that it can vary 1 cm over the day. I have never measured 1m75,4, until today. I ask this question because my diet and exercise habits durin…