Biochemistry and Molecular Biology

Hi all I have a question concerning transcription Problem goes like this: If RNA pol transcribes DNA from right to left, write newly sinthetised mRNA along with orientations. This is DNA: 5'-ATATCCCTAATCCG-3' 3'-TATAGGGATTAGGC-5' I understand that RNA pol can't go from left to right in direction 3'-5', thus we are talking about transcribing DNA which goes in 5-3 direction. So this is what I got a mRNA, and final solution to this problem: 3'-UAU AGG GAU UAG GC 5' Is this correct?

Hi all, have you ever tried to re-run an agarose gel after the run is finished and after visualizing it under UV? Last time I run a gel for a hour but the bands were not separate properly yet. I made the gel again but maybe (for a next time)it is possible to put the same gel on a second run after the analysis? Or does it get degraded?If yes, can you also explain me why? Thank you very much. Silvia

Green Grapes that are ripe and soft seem to taste more sour( tarter), while the stiff ones are much sweeter. How is it unlike other fruits grapes become tarter with ripening? Or is it to do just with enhanced tartaric acid and not actually the sourness?

Or the thermogenesis in brown adipose tissue. Can someone explain me the core of it and how is it connected with respiratory chain and ATP synthesis. Thank you!

Dear all, I have a basic question. I have constructed gene regulatory network for a bacterial pathogen based on expression data. In resulted network high degree of co-expression was observed between TFs and their corresponding targets. Can I come to this conclusion that as a result of such a considerable co-expression this bacterial species can better adapt to harsh conditions and can out-compete other bacterial species in for example co-infections? I will appreciate any explanation Nazanin

My biochemistry textbook says that "insulin increases glucose consumption that leads to elevation of G6P concentration".. But I'm failing to make a connection right here... Glucose consumption by what? by cells? Or is it about the glucose being absorbed in the glicolysis chain where glucokinase elevates G6P levels?

Hello everyone In order to write a paper, I need to understand the "precursor-product model" formula in order to calculate the FSR of mixed muscle protein. In articles using marked phenylalanine as a tracer, this is the given formula [math]FSR=\frac{\Delta E_p}{t} / \left[\frac{E_{M1}+E_{M2}}{2}\right]\cdot 60\cdot 100[/math] Where Delta(Ep) would be the increment in protein bound tracer, and EM1 and EM2 being "the phenylalanine enrichments in the free intracellular pool in the 2 sequential biopsies" and t being the time. I've got 2 questions: Can someone clearly explain to me EM1 and EM2, and why one should divide Delta(Ep) by the mean of EM1 and EM2…

Brucellosis in human can cause by B. melitensis, B. suis and B. abortus. Can anyone explain why Brucella melitensis is the most pathogenic in human? Thank you guys..

Hi all, I've a query about what stage fluorescence is measured at in a digital PCR reaction. Is it continuous or only at the end of the reaction? i thought the former, but I'm after reading a paper stating the latter in a matter of fact way. Can it be both?? Thanks guys!!!

hello i do not know if this forum is the right place but i am looking for researcher or molecular biologist in the study of DNA and how the role of the chromosomes involved with different cell activities depending on the type of human cell. where can i find such a specialist which could provide a service in the analysis of sample of human cells.

What're the top biochemical materials for snow i.e. cover for a motorcycle. Which materials would leave the least buildup? Which materials would likely lead to the snow basically sliding off, or at least a close second? Cheers

Is the coding strand of the DNA always the same as the leading strand being replicated? Or is there no correlation what-so-ever? My question arises from the incredibly more elaborate synthesis process occurring on the lagging strand. Sure it's being copied in the opposite direction and needs to be moved about to accommodate that fact, but it seems to be even more than that to me.

Why do some lesbians look like men without beard and some gay men do look like women? What's the biological reason? It seems for me that there is something biochemical and genetically going on.

So I figure increasing cellular PCr levels, say 10x would be very difficult to carry out and manage...i.e. synthesizing extra enzymes, getting extra nutrients etc. But if it were possible, would the individual be stronger, or just have ridiculous endurance? ~EE

I've been looking at the Michaelis-Menten constant, Km, being zero from a mathematical perspective and it doesn't make any sense - i.e. it breaks the Michaelis-Menten equation (the velocity of a reaction becomes undefined). But does having a Km=0 make any physical sense? Is it possible - does the model need amending to take into account zero values? Thanks for any insight.

I'm interested in supplementing my fitness with L-Argenine (nitric oxide). I've done a little reading (Oh wow look...an uninformed lay person who has read "a little bit" on a topic) about how nitric oxide is a vasodilator, which lends to its effectiveness. What I wonder though is how effective NO is when there is presence of a vasoconstrictor, such as caffiene in the sysm. Does consuming caffiene inhibit nitric oxide's effectiveness?

Maybe the questoin is simple but i couldn't find an answer in clinical textbooks. organic acidemias are related with amino acid catabolism and there's a block in catabolism pathway. so if the amino acids can't degrade to their end products such as pyruvate or acetyl coA, where the ketone bodies seen in these disorders come from?

Hi all, I am currently writing up an essay on Niacin and Vitamin B12; I am focusing on niacin currently. Part of the marking scheme asks for information on a specific metabolic pathway so I was going to talk about the process from tryptophan to nicotinic acid. The essay is to be 1500 words so not too much but how should I write about a specific metabolic pathway without just directly quoting the order of the diagram? I have included the diagram that i have within my essay. Is there a more exciting way to write about this?! and would this be the best metabolic pathway to discuss? Does anyone also have a suggestion for a good pathway to talk about when I co…

Hello can anyone tell me do the chromosones or nucleolus play a role in the context of an itch ? Do the chromosones produce any complex molecules which triggers the activty of the neuron cells ? And then there is a reversal process which stops the itch ? Do the chromosones or nucleolus play any role whatsover ? Thanks

Hi all, Im revising for my upcoming end of year exams and the 20 mark questions are stumping me...not sure what else to include. The question I am currently answering is 'Using suitable examples explain how anabolic and catabolic pathways connected within metabolism'. I understand what the questions means and I have given roughly 12 points worth but unsure what else I can add, therefore my question is can anyone indicate what I have missed please. The points I have made so far are the definitions of both catabolic and anabolic, which uses energy or produces it and in what forms, an example of both types of pathway. I have also mentioned amphibolic pat…

Can you get serotonin syndrome with the use of a highly selective serotonin agonist alone? without the use of SSRI's. And when do you know you have serotonin syndrome? from personal experience mostly all highly selective serotonin agonists, of say the the 5-ht2b receptor give you the same symptoms as that are described as serotonin syndrome i.e (increased heart rate, sweating, dilated pupils, comeup shits etc) are all generic symptoms of highly selective agonists without the use of SSRI's. So wheres the threshold to know when your in danger? And can dopamine RI's or RA's effect this syndrome or is it only relative to serotonin agents.

Hello and a happy new year, I have a question about the expressed sequence tags (EST). I'm given a specific gene with its name, aminoacid sequence and nucleotide sequence and I need the expression frequency of the linked EST's. Is there a database that gives informations about the EST expression frequencies? The genes are from the algea Chlamydomonas. Maybe somebody has an idea how to solve this problem. Greetings, Bello

Hello! I was reading about an experiment about how chopped up potatoes release more catalase from their cells when placed in a boiling tube containing hydrogen peroxide than a lump of potato. Why is this?

Due to stomach irritation of high doses of EGCG, I would like to use it transdermal. This is the extract I consider buying: https://liftmode.com/green-tea-extract-300-grams-10-58-oz-98-polyphenols-50-egcg-fblm.html#product_tabs_nutrition_facts So the questions are: 1) Would it be enough to simply dissolve the powder in simple oil and apply it to the skin or would I need something like Emu oil or DMSO to carry the EGCG into the bloodstream? 2) Since the EGCG absorbs directly into the bloodstream, does it bypass the liver altogether? Would this way of application avoid any (liver) toxicity? Thank you, Ben

Good afternoon, We have a been working on a phosphatase, which gives indifferent over expression levels in E. coli using a T7-based system. We also have a triple mutant variant which also does not express well. Both proteins are trickier to purify than other proteins with which I have worked, because they seem prone to precipitation. We have not yet found conditions which reliably keep them in solution at concentrations above roughly 2 mg/mL. More recently we were given a plasmid with the phosphatase as a GST fusion, and it over expresses well. We are wondering what is likely to be the best way to obtain a gene for our triple mutant as a GST fusion. We see two b…