rakuenso

Just tested 45Hz, for some reason it works quite nicely as well

Were you playing any music?

audacity I'm actually looking for a program that can shift through a series of frequencies 10 hz at a time so i can find resonance easier

what the hell would you NOT want to make that for? sometimes you do things for the hell of it its like asking a wiles "Why woud you ever want to solve Fermat's last theorem?"

its either going to be either definately america or definately not america

http://www.nottingham.ac.uk/~mbzspd/methods/DNA_Sequencing.html Here's one that lists reagents and buffers

if its just simple dideoxy kimball has a nice explanation on it: http://users.rcn.com/jkimball.ma.ultranet/BiologyPages/D/DNAsequencing.html

are you using the dideoxy method or shotgun method? and what kind of sequences are we talking about? Genomic? Plasmid? or etc...

I just made a 70Hz sine wave on the computer, it was pretty kick ass. Everything in my room shook on a standard 120W subwoofer Anyone else want to give it a shot? http://ancient.ssforum.net/70HzOwnage.wav Note: Don't bother if you don't have a subwoofer

that's why I always bring a plant whenever I go into a room

et nique ta mere!

By vitamin C do you mean soley ascorbic acid? I think (not verified) synthetic vitamin C is purely ascorbic acid and natural might be a bit more heterogeneous in terms of other compounds in might contain

ok thx =)

hehe mokele forgot to add the tag "unless its about why creationism should be taught in science classes"

oh.. i counted that as an intuitive approach

as stated, is there a none intuitive way of solving [math] cos(x)=cos(x+pi/2) [/math] I know from experience that 7pi/4 and 3pi/4 are the solutions, but is there a way to derive it?

is VB a one time fee or a yearly subscription fee?

Only in this exceptional cold will helium turn into a liquid (at −269 C). Onnes achieved this feat first. He received a Nobel Prize for his efforts. Man how the bloody hell do you turn helium into a solid then

here's what I did: first switch x and y [math] x=e^y/(1+e^y) [/math] [math] ln x = y - ln(1+e^y) [/math] [math] ln x + ln (1+e^y) = y [/math] [math] x + xe^y = e^y [/math] [math] y = lnx - ln(1-x) [/math]

HYPoThAlAmICoHYPoPHYSeAlS is the longest word that can be spelled using chemicals

if cells didn't die tadpoles wouldn't become frogs and we'd have webbed cancerous fingers

Times UK Kansas schools take step against evolution By Sam Knight and agencies Teachers in Kansas will have to spell out specific objections to Darwin's theory of evolution under a new set of teaching guidelines approved in the midwestern heartland state last night. In a major success for proponents of "intelligent design" and other creationist theories of evolution, the Republican-dominated Kansas Board of Education ruled by six votes to four that, from 2008, teachers will have to give reasons why Darwinism is just one of many theories to explain the origins of life. Until yesterday, Kansas had allowed teachers to take issue with the theory of evolution. Now they will be forced to do so, using an official list of perceived weaknesses in Darwin's theory. Kansas has a history of defying evolution. Notoriously, in 1999, the state deleted most references to evolution in the science standards, a decision that was substantially reversed in 2001. Critics of the new science standards say that that all the objections listed in them derive from "intelligent design" (known as "ID"), a theory that maintains that the life is irreducibly complex and must have been created by a higher power. "All the arguments inserted in the standards are only found in the literature of intelligent design," said Jack Krebs, a high school maths teacher and vice president of Kansas Citizens for Science, which opposes the change. "Teaching the criticisms is teaching ID." Eugenie Scott, director of the National Center for Science Education, said that the tactic used by the creationist lobby in Kansas - to portray criticism of evolution as part of encouraging diversity of thought - is likely to imitated in other parts of America. In August, President Bush endorsed teaching intelligent design alongside evolution. "This action is likely to be the playbook for creationism for the next several years," said Mr Scott. "We can predict this fight happening elsewhere." But supporters of the new regulations say they will lead to open discussions. "We are being very brave. We are brave enough to have all areas discussed," said Kathy Martin, a Republican member of the school board who voted for the change. "Students will be informed and not indoctrinated." The Discovery Institute, a conservative think tank, said that the new science standards in Kansas will led to a fuller understanding of evolution. "Under these standards students will learn more about evolution not less as some Darwinists have falsely claimed," said a spokesman for the institute. http://www.timesonline.co.uk/article/0,,11069-1865033,00.html

there really hasn't been enough study done to say that anti-oxidants is the gem in the quest for longevity. There's a reason as to why we die when we're suppose to die.... millions of years of natural selection has made this very evident.

The problem with DNA electrophoresis is that you can never be sure if the gene is actually going to be expressed. I'm not sure if plasmids also follow similar promoter/operon/repressor commands similar to the that of normal "DNA" and if plasmid DNA will always be expressed (not my field =) ) I guess you are right with the excess effort involved though.

Why can't SDS-PAGE be used? You would have a marker that tells you the relative position of the Beta-lactamase gene, and if it is successfully integrated into the genome such that it's expressed, you should be able to detect beta-lactamase via SDS-PAGE after lysing the cell and purifying it with centrifugation?