Medical Science

Whats an advanced textbook on Nutritional science or sports nutrition for people who already have a solid understanding of biochemistry. Recommendations much appreciated!

Hi, I'm using the Qiagen miscript rtqPCR kit for small RNA quantification. I did a gel purification for RNA bands >40bp and those <40bp. When I then performed miscript with miRNA specific primers, I still had a strong signal ~24 ct for the >40bp gel fraction. However, melt curves and pcr products looked identical between >40bp and <40bp. Could it just be my gel run wasn't efficient in separating the bands? Seems hard to believe. Any insight would be helpful, thanks!

Does anyone think intracellular pH is an important physiological factor that regulates cellular function? The STAP papers have been retracted but perhaps intracellular pH may still have physiological relevance outside of stem reprogramming?

Wikipedia says that meldonium inhibits γ-butyrobetaine hydroxylase (IC50 = 62mcM), carnitine biosynthesis, and beta oxidation. How does this mechanistically link up with improved exercise tolerance in stable angina (and athletic performance)? Supposedly myocardial work is reduced decreased by the lack of beta oxidation feeding acetyl CoA into the Kreb's cycle, and this improves cardiac endurance?

I want to do a study of medical records... How do i get permision for it ? (I am not a doctor)

In 2007 I broke my femur and had a titanium rod put in (through my knee), held in place with titanium nails. I went to a "knee specialist" (yeah, I don't know, guess in Florida, which is where I happened to be at the time, they like to specialize in things like 'just knees', probably because there's so many people down there, they have to break the work load up; "Hey, if I take knee patients and you take back patients, that'll free us up for golf on Thursdays" ) recently to see if anything needed to be taken out, as I was starting to have a lot of pain in my knee where the 'nails' are. He basically told me "if you want to deal with the bills and hassle involved with s…

Sup people, I want to ask if anyone has experienced any growth after age of 19, I'm struggling with my height and I want to know all the possible choices I have to force some growth if possible! Share some of your stories here, or links, anything you have.. Reason to believe I could still grow? 1.I've been the same height since 14-15 (maybe gained 0.5 inches since) 2.I don't have a facial hair and I don't see it comming too soon 3.I have the build of a junior high kid If I won't be able to gain about 2 inches(atleast) I want to kill myself, I'm already researching on how to. So please no troll.

Super Bowl 50 Live Stream

Hi all, If anyone has had experience with preparing and dosing prednisolone, I would greatly appreciate your advice on a few things. I am looking to prepare prednisolone in 0.5% methyl cellulose - preferably at a concentration of 2 mg/mL. Does anyone have experience using 0.5% MC as a vehicle for prednisolone? How is prednisolone’s stability in MC? Also, could you tell me how often you prepared the prednisolone solution for dosing? Any information would be great. Thanks!

I have a pretty crazy idea, that probably won't work, but it's still worth thinking about. So you know with how we have to go to school, and learn. How cool would it be if we could "upload" information into our brain. I don't know how we would do that. So I have a few questions. When we learn something, are the things we learn put into our memories(like what we ate yesterday), or does what we learn go into a different part of the brain? And what are memories anyways? How does the brain store memories? And are what we learn memories? How are things like learning karate or gymnastics stored in our brain? Is that stored in our memories, or is that a w…

Why would somebody not develop antibodies after vaccination/infection??

Hello everyone, Lets say there is a guy with colorectal carinoma and he developed metastasis in liver. 1. My question is now this guy has a liver cancer as well. Does this liver cancer contain dysplastic cells from colon or does it contain dysplastic hepatocytes. In simple words what I'm asking is in metastatic liver cancer is it the liver cells that are growing out of control or is it in this case cells from the colon has got implanted in liver and this colon cells are growing out of control in the liver. 2. If it is colon cells, does that mean metastatic cancer of liver would not affect the function of liver that much because liver cells are still normal. As opposed…

Does anybody know any websites that will teach me about conjugation in the body?

Hello everyone, I'm talking about retaining urine in the bladder,not renal failure, so I can't think of uraemia as a course. So what is the reason behind this. Thanks

Hello everyone, How does Lymphoedema cause ulcers? Example people with filiariasis having skin ulcers.

So I recently heard about this syndrome which states that there is some sort of a problem with chromosome 17. But also that it is typically not inherited but occurs in problems with reproductive cells (sperm or egg) or in early fetal development. How would such a mutation occur?

Why is that usually, Diarrohea due to large intestine problems usually cause blood and mucus diarrohea While diarrhoea due to small intestine problems usually cause watery diarrohea. Thanks

Hello everyone, From what I have read I have deduced that both bowel peforation and strangulation have same clinical features, which are fever, leukocytosis, pain, tenderness, increase in vital signs (eg tachycardia) 1. Why is this? Is it becase strangulation bowel (which means necrosis really) eventually lead to peforation. If not how do you differentiate between the two? 2. If bowel peforation occurs, peritonitis is bound to happen, so are these clinical features due to peritonitis. If not why do these two things cause these clinical features. Eg why cause fever? 3. If some abdominal organ burst, peritonitis can occur. If a thoracic organ occurs infect…

Hello everyone, Charcot's triad is there to describe the clinical features of acute cholangitis. They are 1.Fever 2. Right upper quadrant pain 3. Jaundice 2 and 3 in charcot's triad is confusing to me. As I want to know if they occur due to cholangitis (infection) or due to gall stone obstructing common bile duct and then getting infected causing cholangitis. Is this charcot's triad made assuming that a gall stone causes cholangitis, or even without a stone would 3 features mentioned in Charcot's triad occur in acute cholangitis. Thanks

A question that was asked on a previous edition of the exam concerning the cardiovascular system, was: Nose decongestion, hypertension and mydriasis may be evoked by a Parasympatholytic Sympathomimetic Parasympathomimetic Sympatholytic Of course, the last 2 are false. Now, there is a big discussion on which of the 2 remaining would be the correct answer. I plead for the sympathomimetic, whereas others prefer the parasympatholytic. Arguments for parasympatholytic: Removal of the sympathetic inhibition by the parasympathetic nervous system Inhibition of the parasympathetic effects by the vagus nerves on SA and AV nodes, increasing heart rate Arguments for sym…

Hi everyone An aspect of my course of energy and metabolism is the influence of cyanides in our body. This is what I've written down during the course, which is confirmed by Wikipedia: Cyanide ion binds with the iron-cation in cytochrome oxidase complex (cytochrome a-a3), inhibiting its possibility to continue the electron transport (the reaction 2 cyt a3-Fe2+ + 1/2 O2 + 2H+ <--> 2 cyt a2-Fe3+ + H2O is inhibited), leading to suffocation. I understand that the blockage of this electron transport inhibits a normal ATP-production, leading e.g. to a reduced or completely inhibited function of the Na-K-ATPase, eventually leading to depolarization, eventu…

Hello! Have a few questions about cancer. Would appreciate in depth explanation if possible since a family member was recently diagnosed with prostate cancer. Do you know if it in theory would be possible to starve away cancer by fasting (Only water) over a longer period? Cancer cells depend on much more glucose? Or will the cancer cells get what they need anyway? Can they then switch to glutamine or something else and still spread? In the studies i have seen they use short term fasting + chemo. On a website they claim fasting on nothing more than such things that cancer don´t like - grapes, curcumin etc can cure cancer. They also mention that mixing something like c…

Let's say there is a bowel perforation and this results in sepsis, I know surgery would have to be done or else the person would die. But my question is , is the person first given aggresive IV antibiotics first and when infection is controlled only surgery done, or in emergency situations like this it is done asquickly as possible as there is no time to wait. Why I ask is wouldn't general anaesthesia be hazardous in a sepsis patient. Also can't the surgery further disseminate the infection if it not resolved first. Do they just take the risk because there is no choice?

Hello everyone, How does this occur. I'm talking about retention (eg urine stuck in bladder) not the pre renal failure which myocardial infarction can obviously cause. Also while we are at it, I also read diuretics can cause retention of urine also. That's interesting because that defeats their purpose in the first place. So how do diuretics also cause urine retention. Thanks

Hi everyone Most of you have been the lucky ones to have received a blow to the stomach (right under the proc. xyphoideus), whether on purpose or not, and first of all, I was wondering if it hurts with you guys as bad as it does to me. To clarify, it's an extreme pain that causes nausea, dizziness, extreme sweating (in short, for all males here: same symptoms you might experience when getting a blow to the scrotum). If not, then I shouldn't ask my next question and well... Not consult a doctor since it's been extremely painful for all my life. I mean serious, just pressing on the top of my stomach (let's call it epigastric region from now on) even feels uncomforta…