Microbiology and Immunology

don't know how many years already I had my sinus infection, when I'm 12 years old already as much as I remember, 17 now, and still has it until today, does this disease can't be cured at all? I mean, I'm tired of this already, especially when it caused me having flu or cold like once / twice a month! >.< so, If I'm having flu I'm taking pills, make it gone, and I don't need to sneeze every 5 minutes or having my nose look like "cave's leak" are there any side affect of taking this pills regularly just to remove my flu "temporary"?(well, this pills will lose it protection ability after several day) p.s. if I put this on wrong forum, mind to move it

I am in a summer microbiology class that only meets once a week. We were given a random unknown bacteria and have to figure it out. The possibilities for this random bacteria are 1. Eschericia coli2. Enterobacter aerogenes 3. Staphylococcus epidermis 4. Streptococcus salivarius 5. Clostridium sporogenes 6. Serratia marcescans 7. Kocuria rosea 8. Klebsiella pneumoniae 9. Mycobacterium smegmatis 10. Proteus mirabilis 11. Proteus vulgaris 12. Sarcina lutea 13. Pseudomonas aeruginosa 14. Lactobacillus acidophilus After doing a Gram stain and observing it under the microscope I discover…

AtlasT4SS: A curated database for type IV secretion systems. http://microbes-human-health.blogspot.com.es/2012/08/atlast4ss-curated-database-for-type-iv.html

Dear all, Can you help me on this matter? 0.1 ml of inoculam is added to the 10ml of Rappoport Vassiliadis medium but 1ml of inoculam is added to the selenite systyne broth. What is the reason to add less amount in to RV medium? This is regular test I am doing in lab but I need clear idea on what I follow.

Hello, I would like to get suggestions and tips on which methods I should use to remove completely the endotoxin on a recombinant protein expressed in E.coli. In fact, the protein I've purified was found to be contaminated by LPS. As I intend to use it in studying the immune response of Bone marrow-derived macrophage and other macrophages cell lines, the contaminating LPS will interfare with my results. I plan to use the Detoxi-Gel removing gel from Thermo scientific. Will anyone tell me, what I should expect as result (the kit ensure to remove 1 ml of gel removes >9,995 EU from a 10,000 EU challenge of LPS (greater than 99% efficiency)). What is the minimal endotox…

Whole Genome Deep Sequencing of HIV-1 Reveals the Impact of Early Minor Variants Upon Immune Recognition During Acute Infection. link removed

Dear friends In my research i need to isolated DCs in popliteal lymph-nodes and then culture them by LPS and then IL12 assay by Elisa. I used Anti CD11c magnetic beads and autoMACS to isolate DCs. My Elisa results shows even in culture of DCs with No Ag we can detect high level of IL12 production! Is it possible it be for magnetic beads?? any recommendation? Using cell sorter is not practical in this case because cell number is very low. hope to hear you soon. Thank you very much Masoud Akbari Nagasaki University Japan

Hey, I am an almost beginner in the field of Immunlogy and try to get rid of red blood cells by cell lysis after suspending whole spleens. The buffer I use for this purpose is a standard ACK buffer and I incubated previously for 5-10min on ice. Unfortunately, the only thing I get out of it is a pellet of debris. 1. is the incubation time really too long? 2. is it possible that the buffer decays by sitting on my lab bench for a few months? Thanks

Hello fellow scientists!! I am the lead researcher of a group that is trying to produce a functioning tissue-engineered ligament for anterior cruciate ligament regeneration. Anyway... we are currently using a rat model and implanting "scaffolds" (basically a polymer construct) with cells seeded onto them into the knee capsule. I am trying to figure out how to "track" the cells to evaluate survival and proliferation after we sacrifice the animals. By "tracking" I mean differentiate between our implanted cells and the host cells that infiltrate our construct. There are two transplantation models I am trying to figure out: 1) Rat cells into rats. We have GFP …

Dengue disease is so old.it has root in jin dynasty china 320 AD.but no treatment have been discribed yet.i thnk y thre is no treatment

Hi! I'm currently studying immunology and am quite confused about how our body can eliminate an viral infection. As far as I've heard, the dendritic cells are important in this because they can present extracellular patogens as MHC class I and thereby activate CD8-T-cells. But how could that eliminate the intracellular viruses when the dendrittic cells reacted on an extracellular patogen in the first place? Or is the explanation that the extracellular fragment could be a viral particle? Furthermore, how could MHC class I activate B-cells to undergo differentiaton to plasma B-cells? Is it so that we don't have antibodies against virus, and that they are prima…

Are the large number of microorganisms found in the mouth a cause for concern? Explain

One of the episodes of a TV show I like to watch (Fringe, season 2) was about a oil drilling operation resulting in the release of a deadly (to put it mildly) virus which one of the characters hypothesizes might have been responsible for the eradication of the mega-fauna of the last ice age. While the idea of accidentally uncovering a dormant virus buried deep underground does sound at least somewhat plausible in principle, I strongly suspect that the 'cure' the Fringe team come up with is just a load of nonsense, but I wouldn't mind a second opinion. The scientist discovers that what ultimately rid the world of this doomsday virus 75 000 yeast ago was the last supervolca…

Please give me a hand in interpreting in close to layman's tems this journal.....Thanks Regulation of D-cyclin translation inhibition in myeloma cells treated with mammalian target of rapamycin inhibitors: rationale for combined treatment with extracellular signal–regulated kinase inhibitors and rapamycin Abstract We have shown that heightened AKT activity sensitized multiple myeloma cells to the antitumor effects of the mammalian target of rapamycin inhibitor CCI-779. To test the mechanism of the AKT regulatory role, we stably transfected U266 multiple myeloma cell lines with an activated AKT allele or empty vector. The AKT-transfected cells were more sensit…

Is there any published information about the differences between TRI zol by invitrogen and Tri reagent by sigma?

hi immunologists. my goal is to expose the myth of vaccination (or the myth that it is some robust method of granting someone immunity from "disease") please in laymans terms (or with as much nomenclature/jargon as you want) lay out the vaccination "scheme" if you will, like the logic or thought process behind it. thank you for your time and your explanation.

Hello. I am conducting an experiment looking at secondary metabolite production though out the growth phase of a culture. I will need to take out fairly large volumes of culture over a period of 2-3 weeks to determine both microbial dry weights and metabolite concentration. In the past I have used a repeated measures design, i.e. repeated sampling from 4 large replicate flasks, never taking more than 10% of the original volume. However this most recent experiment using dry weights will require me to sample larger volumes and there is no way I can realistically use larger flasks. Instead I was planning on using many small flasks, and at each time point sacrifici…

Hi, i did my first microscopy practical last week and im just writing up my rough work now... However, i've realised i didnt record which magnification i was using! High school error, i know.. Anyway, I was measuring the head of a hog louse.. The measurements i scribbled down say: 3 Ocular units/ 75 micrometres I have also made a note of: 100 x 30 Stage units... (Dont even remember writing that, but it's here, so i obviously did. Dont know what it means though) Not sure whether this is of any use, but the specimen data sheet says actual size of a hog louse is usually 4-6 mm. (Complete size, obviously, not just head size, which is what i was measuring.) …

Hello, I recently saw one-way MLR in my course immunology. What is the difference between a one-way and two-way MLR?

I need to determine the MIC of some products that are used for filling teeth in dentistry... those products consists of different ingredients, that provide physical and chemical properties of the products... One of my goals in my study is to determine the MIC of those products... I have the products but not the raw ingredients... My Question is how to determine the MIC of those products? I know that I need to make a serial dilution of the product but this may not be possible...

Does anyone know whether it is possible for someone to get Herpes from a toilet seat? I remember watching an episode of House once in which he tricked a guy into admitting he cheated on his wife and got HSV because of it by saying that he in fact could have gotten the virus off of a toilet seat. Now, obviously in the episode it was all a ploy, but I can't help but wonder whether it might be THEORETICALLY possible to acquire a productive HSV infection in that manner. My intuition says that if the MythBusters were ever to put this to the test they would most likely deem it something like 'Plausible but ridiculously unlikely', or something similar. Does anyone know?

I am looking for someone with knowledge about pentraxins. I was wondering if there is anyone who would read my Bachelor thesis, to give me at least an idea if got it right or if I am totally on the wrong track. If yes I'd like to send you the file per e-mail. Stephanie

To me, it looks like some sort of branching fungus. Anyone able to identify what this could be? It was originally discovered growing inside a plastic container in my bathroom that was filled with some old earrings that were not fully cleaned. I am assuming the dead skin cells are what it was feeding off of. After stumbling upon this, I transferred some of the spore-like pieces into a plastic vial with some of the (for lack of a better word) "gunk" from the earrings. The fungus grew at a fairly quick rate; there was a noticeable increase of them within just a few days. ....Excuse my amateur vocabulary -- I have not taken Microbiology yet in school!

Hi there. I've been doing some biochemical tests on unknown microorganisms. They're from a UK kitchen, so nothing too exotic! I was just wondering if I have sufficient evidence to convict; 1) a microorganism as Bacillus spp with the following: Gram: positive rods, spores visible Catalase: positive Oxidase: negative MacConkey: negative 2) a microorganism as Pseudomonas spp with the following: Gram: negative rods Catalase: positive Oxidase: positive MacConkey: negative Urease: negative 3) a microorganisms as Klebsiella spp with the following: Gram: negative rods Catalase: positive Oxidase: negative MacConkey: positive Urease: positive …

Dark green,corrugated colonies on sabourauds dextrose agar appear within 1 week.Lactophenol cotton blue mount shows the picture given here.I need to identify these fungi.so,kindly help me.