svenson

Hey, I am an almost beginner in the field of Immunlogy and try to get rid of red blood cells by cell lysis after suspending whole spleens. The buffer I use for this purpose is a standard ACK buffer and I incubated previously for 5-10min on ice. Unfortunately, the only thing I get out of it is a pellet of debris. 1. is the incubation time really too long? 2. is it possible that the buffer decays by sitting on my lab bench for a few months? Thanks