Genetics

hi DrDNA I am currently working on the 100bp DNA ladder (dsDNA) and it should produce 12 peaks on the CE. yesterday the profile showed 12 peaks!!!but all students in the lab are facing the problem of reproducibility, the result is not reproducible. After i got the sucssessful result I ran the same sample once again under similar conditions and I only generated one peak!! and I tried again after i changed the buffer and still one peak shows up. By the way I am running the ladder in buffer only without polymer. what could be wrong, I cannot get reproducible result for the same sample under similar condition??!!! which is very strange. if that would help you think with …

hi all; I am currently working on SSCP, capillary electrophoresis to separate DNA sample from multiple bacteria (extracted from animal feces). The profile does not show all the peaks expected but just few up to 7 peaks. I am expecting unlimited number of peaks to show up. the procedure I used is this: -1:18 dilution with Tris Buffer -denature for 5 minutes at 94C -cool on ice for 5 minutes -add the dye and leave for 5 minutes the electrophoresis condition: -inject the polymer -wait for 10 minutes -inject a low current (1KV) for 5 minutes -inject the sample (at 10KV, 60s) -separate the sample (at 12KV, 150 min) please if you can help email me back …

hi all! i'm a little bit confused regarding the EGF receptor signaling pathway. if there is a suspicion that one component of the pathway controls the expression of a gene but when a null allele of the receptor is used in a transgenic animal (a mosaic animal, if that matters) so that the cells in the clones have no receptor, wouldn't this mean that the signaling pathway is not activated at all in these cells? and if it indeed means that removing the whole pathway has no effects on the expression of this gene, how could components of the pathway downstream of the receptor be suitable candidates for the regulatory function? thanks in advance for any advice…

I was talking with my friend who is a 5th year med student...and we were discussing which of the two gender are better than the other. Apparently females have better genes than men leading them to suffer from less diseases and live longer and much moer which can't come to mind right now. I thought this to be more of a social and environmental impact on the male species not a genetic one which will keep on continuing. Your views please

http://http://www.foxnews.com/story/0,2933,299857,00.html Well this link is the story if you haven't read it already. This part of the story that gets me...The man-made chromosome will be transplanted into an existing bacterial cell and is expected to take control of the cell. When the synthetic DNA takes over, the cell will be a new species. Look if they created their own bacterial cell and put the DNA in then i think it is eligible to be its own lifeform......otherwise it is just theory working and working well.

This idea came to me this morning. I haven't had too much time to let it sink, so take it will a grain of salt, but use it as seed for the imagination. If you look at the DNA double helix, it is composed to two sets of base pairs. So essentially, the genetic code along the double helix is a type of binary coding, where the dot is one base pair and the dash the other. The nuclei acids or bases within the DNA are resonance structures. What that means are the electrons are delocalized so they share with the entire ring structure. The easiest example to see is benzene. It has six CH, that form a ring structure. At any one time, there are three double bonds and three sing…

My friends , do you know which forum is about the quantity genetics where everybody can communicate the quantity genetics ?

I have dark blonde hair (dirty blonde) naturally, with hazel eyes, my ex has brown hair and brown eyes, my daughter is starting to get dark blonde and greenish eyes.. i thought that brown was the dominated one from green? Isnt my daughter supposed to have brown hair and brown eyes? or is she going to look alot like her mom? i need some answers here. thanks

Well, i know that we all have genetic idenetities from our parents. However, my question is, for example, i have a not so intelligent father and mother, and i turn out not so intelligent too as a baby. But, when i grow up, i learnt lots of things, have an expanded knowledge. Which i can say, i turned smart after learning.. which is caused by environmental factors. So, would this change in intelligence also change the gene in my genome (accounted for the part in intellignce) to change too? So that when i have offsprings, my child would be smart and intelligent given that my husband is intelligent too?

Does anyone know the Shannon entropy of DNA (any representative organism will do)? Or does anyone know how to calculate this with any accuracy or even within the ballpark?

Can anyone tell me what percentage of the population has brown scalp hair and red pubic hair. Or any information about the combinations. Thanks in advance

Are there chemicals that will permanently depigment human hair?

What happens when point mutation occurs in the somatic cell of a person?Of course the mutation can't be transferred,but does it spread to other cells?and does it manifest itself in the affected individual? I'm sorry if this is a stupid question but I've asked two of my teachers and they don't seem to get my question.

hi all; I found a definition for genus Prevotella saying that it was under the Bacteroides species but after 1990 it became a separate genus from the bacteroides. that's understandable!!!!!!!!!!!!!! Using Bacteroides as a marker to identify the nonpoint and point sources of fecal contamination in the water, in these literatures they always write Bacteroides-Prevotella marker. what do they mean by that? do they mean that both the Bacteroides and prevotella species are used as markers or they still treat Prevotella as one of the Bacteroides species? I would mostly believe the first assumption!!!!!!!!!! your clarification is highly appreciated thanks

A team of researchers has discovered that a bacterial parasite (called Wolbachia) can insert almost its entire genome into the genomes of members of one host species (a fly called Drosophila ananassae), and can insert parts of its genome into the genomes of members of several other host species. "We've found at least one species where the parasite's entire or nearly entire genome has been absorbed and integrated into the host's," says Jack Werren of the University of Rochester, principle investigator of the study and a world-leading authority on the Wolbachia parasite. "The host's genes actually hold the coding information for a completely separate species." This …

Ever since I could remember, I associated selective advantage with an improvement in genetics. But quite recently, a basic observation seems to indicate that selective advantage can also come from regressive genetics. The easiest example to see is to compare the dinosaurs to the mammals. Most would agree that mammals have more advanced genetics. The data indicates that during the latter part of the dinosaur's dominance, mammals had already evolved, yet the dinosaurs had selective advantage since they remained at the top of the food chain. What it amounted to was less evolved genes having selective advantage over evolved genes. If one was a mammal in that environ…

With the advent or ability of humans to modify or understand genetic code, and such a code being responsible in large for the physiology of an organism what will the future truly hold for such an ability. I personally view genetics of course having to be able to operate basically in the reality that is physics and chemistry overall, that being said what would be available in a reality of total understanding of genetics. Will people be able to modify humans that can live in the vacuum of space, will we have people that are more akin to characters in the X-MEN comic book series for instance? IT sounds far fetched and even laughable, but the reality of understanding how to p…

hi all: In one article i found that they were successful in amplifying 16S out of eubacteria. what is the difference between the bacteria and the eubacteria and prokaryotes? also what is the difference between the universal primer that would amplify 16S gene and the one that is specific to 16S gene? please some one define the universal primer for me? thanks

hi all After i mix the PCR reaction mixture (DNA, primers, Master Mix) i need to add BSA (400ng/ul) how do I calculate how much i need to add of BSA to the mixture. thanks

When it comes to tRNA molcules involved with translation, they each will have a certain anticodon so they can put in their amino acid when the corresponding mRNA codon comes up. But how many different types of tRNA molecules would there be? Because you can say there are 64 (4 x 4 x 4), however are there ones for the 3 stop codons? So I'm guessing either 64 or 61 (i'm leaning more towards 61), but can anyone help me out here?

I've been listening to a series of audio lectures on replication and DNA. It's really cool stuff. But since it's audio only, I fail to get a good visual picture of some the - very complicated - processes. So I'm searching through the net for some good animations, particularly about ribosoms (sp?) and how they synthesize proteins out of RNA. Anyone know of any cool animations?

I saw something interesting so I desided to ask the brains that be: I was watching a program about military training and they mentioned that they have problems with some of their recruits that, even after long training, cannot physically cope with lengthy/long distance runs, and they suspected that it was genetic and that no matter how hard they train they will never be able to long distance run without collapsing. I just wanted an educated opinion on the matter thanks. ~Fool

Please see the BBC article below... http://news.bbc.co.uk/2/hi/health/6923577.stm

hi what are the positive and negative impact of gene cloning? thanks in advance

One gene can generate products that are involved in multiple distinct processes/pathways. Examples of this are plentiful. I was wondering if someone with experties on the area can direct me to literature source where I can find 1. the first example of a single gene/protein with multiple function 2. How such phenomenon complicate how we use genetics to decipher function of a gene. Thanks!! Muchado