Bluenoise

Senior Members
  • Content count

    867
  • Joined

  • Last visited

Community Reputation

10 Neutral

About Bluenoise

  • Rank
    Biology Expert
  • Birthday 02/06/1982

Profile Information

  • Location
    Canada
  • Interests
    Women, Biochemistry, Music, Partying
  • College Major/Degree
    Msc Biochemistry
  • Favorite Area of Science
    biomolecular engineering
  • Biography
    A serious Islander
  • Occupation
    Masters student/TA
  1. Message resent as private.

  2. Precipitating a Compound from a Solution

    The MgCl2 wont dry completely this way, well maybe if your humidity is close to 0%. It will likely not dry past a hexahydrate state (6 water molecules per MgCl2). When I typically buy MgCl2 this is the state that I get it in. At this level of hydration the mass per unit of magnesium is the most stable I beleive, due to being less suseptable to change in water content under typical conditions. You can probably get it alot dryer by baking it 100C for an hour or two after evaporation. (Don't take my word on it though, inorganic chem has never been my thing) You might want to try Ethanol precipitation as well. However the sucess of this will be dependant on the concentration of MgCl2 and how high you can get the ethanol content. (Also how cold you freezer is too).
  3. Drunk knees

    Damn how'd you know? I did eat a Kebab... I don't goto the gym to burn calories anyways, I'm slim enough. I just go for the physical activity.
  4. Drunk knees

    Do you think it counts as excercise? It would save me some time if I could counting it towards my 3 days at the gym a week...
  5. Drunk knees

    Okay so this might seem like a stupid question, but I'm curious nevertheless, and have been unable to find the answer anywhere else. Often when I go out for the night and drink probably a bit more than I should I find that my knees feel weak the next morning. They don't hurt, they just feel weak and stiff the next day and sometimes it carries on for another day. Now I'm curious as to the exact cause of this. Is it directly caused my drinking or indirectly? Like is the alcohol doing it or is it more likely that I'm just overexerting myself running around, stumbling, falling over, dancing, going on long walks at 2am in search of pizza. Ummm yeah.
  6. enzyme problems

    Could you tell us something about the reaction?
  7. A little missunderstanding

    I wasn't aware that high schools specialize to such a degree. Informatics high-school!! I think it's a horrible idea. I can't imagine that they actually want 13/14 year olds to make up their mind about their future profession at such a young age, and so little experience. But that being said their opinion on other subjects is definatley going to be biased. And no most people who do biochem don't become teachers. All most all however do some minor teaching along the way however. I started my masters a year ago and I already teach part of a course, I even have a student working under me. However almost all of what I do is research.
  8. Nothing is removed when bleaching. Bleaching simply oxidizes chemicals that are responsible for colour. Colour is a result of highly conjugated chemical bonds that allow a molecule to absorb and readmit light at discrete wavelengths corresponding to the difference between electron energy levels. Bleaching simply removes these conjugated bonds, usually by attaching an Oxygen molecule to them. I'd assume that the brown colour in wood is either due to certain arrangements of lignin or pigments in the wood. But don't quote me on that.
  9. Electrolysis of water...

    I got this to work really well once with tap water a nine volt battery and some wire when I was a kid. For electrodes I basically opened up a couple batteries carefully enough not to break anything.
  10. Did life begin in space?

    I know quoting myself isn't a good idea... But I just want to apologize for my poor spelling and grammar in that post. I reread it and almost threw up.
  11. Did life begin in space?

    Yeah I don't buy it. First of all life needs a renewing energy source to sustain itself. And I can't possibly thing what they maybe suggestion in serving that function inside a comet. Except for the surface where there might be light from the sun etc. However unfortunately the surface of a comet would be so bathed in radiation that it and a good few feet below the surface would be constantly sterilized. I'm pretty convinced that earth is by far the best place in the solar system for life to form, and I think the chance of it forming in comets and seeding the earth is pretty null. Edit* Now having said that. I think it's entirely possible that comets played a role in seeding the earth with minerals and small molecules water etc that was fundamental to the eventual formation of life. I think it's highly likely actually. The early earth was only a tiny fraction of the mass that it is now anyways. But I think that's the extent of their possible part.
  12. What's your field?

    Biochemistry grad school. However my secret identity is in synthetic biology and genetic engineering. I choose my Biochem projects based on if they allow me to design new techniques/methodologies and constructs. Not based on what I'll discover. Drives my supervisors insane having to explain all these hair brained ideas to them lol. At least they work.
  13. Yeah plus anything with the word acid in it is likely the scare the shit out of people who don't know better.
  14. Cloning a Bacterial mRNA

    I know that has me worried a little bit, DNA runs in a Gaussian distribution on gels unfortunatley. But hopefully the effect isn't that large. Yeah seems like a nice easy protocol I can get an undergrad to do in a day .
  15. Cloning a Bacterial mRNA

    After the first amplification you run the PCR products on a gel. You excise a region far larger than the small RNA. This way you've eliminated any PCR product that resulted from the small RNA. Next you amplify with the overhand to get enough insert to clone.