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Genetics

DNA replication, Mendelian Genetics, mechanisms of gene expression, and related topics

  1. Dear ladys and gentleman, I am very new to genetics, but regardless I got myself into the responsibility of designing a PCR workshop. Now I struggle to find out what PCR-Primers I need if I want to multiply an entire DNA strand, rather than one specific sequence. Can anyone help me please?

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  2. Started by Externet,

    Hello. If a DNA sequence is taken from some tissue of a creature -say liver- is compared to another tissue -say kidney- of the same; are they identical ? Should they be ?

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  3. Started by noha,

    what is meant by compromise in the following context, does it mean to hinder? The heat shock response offers another example of the genetic control of cell activity. Heat shock proteins (HSPs) are produced by all organisms when they are stressed by heat and other factors that compromise their biological activities.

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  4. Started by deappri,

    Can someone help me solve the following question: In man, the two genotypes D/D and D/d are Rh+ and d/d is Rh-. The frequency of Rh- people in the population is about 16/100. 1. What proportion of the children in a series of families in which one parent is Rh+ and the other Rh- would be expected to be Rh+? 2. What proportion of the children from a series where both parents are Rh+ would be expected to be Rh+? 3. Amongst the Rh+ children from part b, what proportion are carriers? I somehow got 84/625 for part a and 1-(84/625) for part b. I'm pretty sure it's totally off, so if someone can help explain this to me, I would apprec…

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  5. Lactose intolerance has prevailed in China for a long history. It is generally believed that more than half of the people in China are troubled by this problem. Some studies even believe that 100% of Asians have some degree of lactose intolerance. Therefore basically what is lactose intolerance? Scientifically, lactose intolerance is also known as lactose indigestion or lactose malabsorption. Too little production of an enzyme (lactase) in the small intestine often leads to lactose intolerance, in which people with lactose intolerance cannot fully digest the sugar (lactose) in milk, so they eat or drink dairy products Diarrhea, gas, and bloating. In plain langu…

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  6. I was looking up why dogs don't live as long as humans, and I found a lot of very sophisticated-sounding answers about things like metabolism and the trade off between maturing and reproducing more quickly vs taking longer to develop but having a longer lifespan (the whole--live fast, die young thing), and that makes sense, but... I saw something on a show..a Korean rom com, of all things...about telomere length and aging, and after researching it, I want to know--isn't that the real cause of aging? Aren't the explainations we often hear about reproduction and metabolism missing the real reason why species have different lifespans? Or is that too simple (or inaccurat…

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  7. Started by observer1,

    ok so chameleons change the color by changing the refraction light to form desires color. So theocratically, if there are some kind of nerve that detect the color of light( possibly cones) and send the signal to the other side and the cells recreate the color input to reflect the same, it could be a very camouflaged animal an possibly invisible.

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  8. Started by noha,

    Hello, I am translating a very short introduction to microbiology by Nicholas P. Money and I don't understand how complement of rna is studied using this technique : This is the context: Millions of copies of each DNA sequence are dotted onto the chip in a specific two-dimensional pattern and the investigator can study which of the genes are being transcribed under a particular set of conditions by adding a labelled solution of the mRNA from a population of cells. The mRNA is labelled with a fluorescent dye, and after this sample has been allowed to react with the microarray, mRNAs bound to the segments are identified from the positions of their complementary…

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  9. Melbourne University has recently received a philanthropic gift of $5 million to begin work on trying to revive the now extinct Tasmanian Tiger. The technology to do so had been there for a while. There are many doubters on whether this is possible, but many refuse to provide concrete reasoning to their doubts. People are even claiming its 100% impossible (again, no concrete reasons given). Including some links below: Thylacine (wikipedia) Tiger! Scientists Look to Revive Extinct Species (including this one because most others are behind a paywall)

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  10. Started by noha,

    Hello, I am translating a book called Microbiology: A very Short introduction, and I don't understand the meaning of arbitrary measures of genetic novelty in the following context: The application of the term ‘species’ is even more problematic for viruses than bacteria and we rely upon arbitrary measures of genetic novelty when we refer to different kinds of bacteriophage.

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  11. Started by noha,

    Hello, I'm working on Microbiology: A Very Short Introduction (Very Short Introductions) and I came up with the following sentence which seems unclear to me The induction of cancer is indirect, with the involvement of a specific viral gene (gene X) affecting the expression of genes in the host cells that control cell division. does the second sentence (with the involvement of ...) is an explanation why the induction is indirect? Here is the full context: The link between persistent infection with hepatitis B virus and cancer is clear from epidemiological studies showing that countries where the infection is most prevalent also show a high inciden…

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  12. The ubiquity and prevalence of viral genes in the genomes of cellular organisms is a measure of the influence of viruses on the evolution of prokaryotes and eukaryotes and we are only beginning to grapple with the origin of viruses themselves. The science of virology is younger than the field of bacteriology. I think it means to discover or to understand not to face or stand against. Source: A very short introduction to microbiology by Nicolas P money

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  13. Started by JoshAldursUK,

    So im wondeing if dna is just . how things are made . so if i picked up 2 sticks and put them together with string in only the middle and left it wind comes and moves it or something falls and moves it or somebody walks on it causes it to just take lie i

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  14. I am confused about this question, any advice would be greatly appreciated :)

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  15. Started by stubbsey12,

    Is anyone able to explain this biphasic amplification that I've been seeing in my positive controls (Green curve)? For reference, this is using TaqMan probe technology, whose targets are simultaneously nsp16 and orf1ab (COVID-19). Please ask for further explanation! I cannot find any resources out there which can explain this..

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  16. I'm working with a transmembrane protein and I'm interested in understanding the folding differences between its variants. I've used AlphaFold and some other tools to generate predictions, but this protein folds in the lipid bilayer, which doesn't seem to be supported. I've read here (https://www.frontiersin.org/articles/10.3389/fmolb.2022.906437/full) that AlphaFold's prediction of membrane proteins is surprisingly good. Can anyone confirm this or explain it? It seems that the protein's secondary structure would be very different in water.

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  17. So, i was reading about the origin of life, and i've understood that one of the reasons why we believe there was a LUCA, is due to the fact that there are a lot of characteristics common to all organisms currently living on Earth. Then i've thought that maybe, the use of comparative genomics has already proven that certain characteristics, e.g. the existence of a DNA polymerase, have had the same origin. I don't really know how comparative genomics work, but i'm assuming that they compare the DNA sequences that code for a certain protein, and if they have a relatively low ammount of mutations between the different organisms, then they can assume that the DNA sequence came…

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  18. Hi there, confused EPQ student here. I'm writing a research paper on genetic modification in cattle and I'm just wondering about the differences between the GM of plants and animals, if there even are any. It wouldn't be a big deal if not, I just wanted to make a clarification. Also if anybody has in-depth methords for any of the following, I would really appreciate your time: Microinjection Retrovirus-mediated transgenesis Embryonic stem cell-mediated transgenesis Thanks in advance!

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  19. Hello. At about 4:53 in this video - https://www.youtube.com/watch?v=-QdtwRJdVsM there is a map of the world with major haplogroups. A lot of Europe and Middle East is in red. Africa is mostly green. However there is an island of red a little above the middle of Africa and a little west of center surrounded entirely by green. What is that area? Which countries and peoples belong in that chunk? What is the connection between that chunk and the red areas in Europe and the mid-east? What are the actual haplogroups, haplotypes represented in the red chunk? Any time taken to respond is appreciated in advance. …

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  20. You have the canonical five (A, G, C, T and U), although I'm seeing articles where it's a little more complicated because there's also a non-standard base of I, although I'm a little fuzzy on when this one appears. There also seems to be a new base, discovered in viruses, dubbed Z. (Because viruses get involved, I'm looking at both RNA and DNA together.) https://www.wired.com/story/dna-has-four-bases-some-viruses-swap-in-a-fifth/ I seem to recall reading this one does appear in certain fungi and not just viruses, but can't find the article on that. Besides which, it's not always reliable to go with one-off pieces, especially outside of the scientific journa…

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  21. Did the original human beings have the genes of every single nationality? Adam and Eve must have had genes common to French, African, Indian, Australian, Chinese people etc to have spawned the human race. On a side note, that must have made them look rather strange. Were they multicoloured, for instance? Can you imagine a human being who is equally Caucasian, African, Asian and European all in one? Adam probably had a pink nose and a yellow face. He probably had brown hands and a black chest. Does this rather curious theory make sense? or in other words... IF, and only if, in BC ad infinitum... THEN, in 2022...

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  22. Мне нужна литература по теме «Особенности устойчивости генотипов риса к абиотическим заболеваниям». And what resistant new varieties do you know?

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  23. Started by ikhanym.7,

    hello everyone! my topic is related to the resistance to leaf rust of isogenic wheat lines. Please tell me which article or book to start studying?

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  24. Hello colleagues! We all know that by studying nucleotide polymorphisms (SNPs) in the human genome obtained using a biological chip, it is possible to identify genes that affect the development of many diseases found in humans. I am interested in whether there are scientists among us who have researched this topic. If yes, I would like to ask a couple of questions: 1. What programs were used to determine homozygous areas of a person? 2. Did you have practical experience and what results were obtained? I will be sincerely grateful for your help! Have a great day.

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  25. What new computer programs are being used to analyze the virus genome?

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