Biochemistry and Molecular Biology

I remember hearing of a chemical, a neurotransmitter to which THC is an analouge of. It was called anadamine or amandamine or amide or something. I have tried looking up all of the variations of what i thought it was, but im not getting any results so i must not have the name right. So thats all im wondering is does anyone know the name, or does it even exist

Anybody know any good acronyms for the substrates and enzymes of the urea cycle??? My test is Friday and I am freaking out!

I know that it is possible to engineer bacteria in order to create biofuels but it is possible to say turn bacterias chemical energy and transform it into useful electrical energy?

In the prophase, the genetic information of a cell, usually long strands floating around in the nucleus like noodle soup, suddenly condenses immensely into tightly bundled, extremely packing-efficient structures. How is this orchestrated so quickly? What molecular mechanisms are at work?

Question 1 The inner (plasma) membrane of E. coli is about 75% lipid and 25% protein by weight. How many molecules of membrane lipid are there for each molecule of protein? (Assume that the average protein is Mr 50,000 and the average lipid is 750.) Question 1 answers 1 50 200 10,000 50,000 Question 2 The shortest  helix segment in a protein that will span a membrane bilayer has about _____ amino acid residues. Question 2 answers 5 20 50 100 200 Question 3 According to the current model for HIV infection, which of the following is not involved in the process of membrane fusion? Question 3 answers A cell surfac…

Q. Hemoglobin is a tetrameric protein with two alpha and two beta polypeptide subunits. The structures of the alpha and beta subunits are remarkably similar to that of myoglobin. Myoglobin is also a heme containing protein that binds oxygen that has a structure that is similar to that of hemoglobin. However at a number of positions, hydrophilic residues in myoglobin have been replaced by hydrophobic residues in hemoglobin on the outside of the individual subunits. a. How can this observation be reconciled with the generaliztion that hydrophobic residues fold into the interior of proteins? b. In this regard, what can you say about the nature of the interactions that d…

Hi everyone! I'd like to measure concentration of NR3C1 in sputum of asthmatics. I suppose real time PCR s the best method and becase of that I have some questions. What do I need to do this? I found primesr for this receptor. http://www.realtimeprimers.com/vhps-1818.html what else do I need to start and where can I read more about how to do this. Of course I have an access to lab but I'd like to read on my own before asking my assistant professor everything. I am a beginer in molecular technics. hope to read from you. best wishes.

Hello, this is my first post. I was wondering if anyone here as any experience working with lymphocytes extracted from blood and checking their viability with trypan blue exclusion. I get the impression that I am overcounting dead cells or that I may be counting red blood cells that are not fully removed after purification. What are your thoughts on the matter? Thank you.

Can you suggest where can I buy Molecular Biology Laboratory Equipment in UK? I only want some general laboratory equipment. Which shop(s) are suggested?

Can anyone please explain the difference between "Inhibiting" a protein and "Allosterically regulating" a protein? Thanks in advance.

I have looked through my notes and book and can't find the answer so any help would be great. "In x-ray crystallography it is imperative that 3D crystals of highly pure protein be grown in the lab. Why is this necessary? In other words, why can't an x-ray source generate a diffration pattern from a single molecule or even 2D crystals?

I just started biochemistry and am very rusty when it comes to buffer calculations.. can anyone can offer me any tips on solving these two problems? Please? 1. What weight of glycine (pk1=2.4, pk2=9.6) and what volume of 0.5N HCl would you need to make 100 mL of 0.2M buffer of pH 2? 2. What weight of glycine and what volume of 0.5 N NaOH would you need to make 100 mL of 0.2 M buffer of pH 9.6? glycine mw=75

http://partsregistry.org/Main_Page Has anyone done anything interesting with this (in real life?) In my bioinformatics class we have to design a theoretical bacteria culture that can process an input and have two different outputs. I'm trying to design a bacteria that glows and smells like mint when the lights turn off. I'm interested to know though, if anyone has done any bioengineering stuff like this. It seems like it has enormous potential is people utilize it correctly.

I have a problem that I cannot figure out. Can someone please help?? Suppose you have a peptide with the sequence: val-pro-ala-glu-ala-cys-lys-met-ile-val-his-ser-leu-arg-glu-gly If you add the enzyme carboxypeptidase and check the concentrations of amino acids every 30 seconds which amino acid will always have the highest concentration?

Welcome to the Molecular Machines A thread to lump together all the interesting discoveries regarding the intracellular biomolecular machinery that are crucial for life to exist. Please post interesting discoveries and perhaps describe the functionality of these intracellular biomolecular machines. Intracellular biomolecular machinery include the following: 1) DNA replication and repair machinery (replisome) 2) DNA transcription machinery and RNA processing and translation machinery (Spliceosomes and ribosomes) 3) Cell cycle signaling network (pRB, e2F, CDKs) 4) Programmed cell death machinery (Apoptosis, autophagy, mitotic catastrophe) 5) Protein process…

What is the most reliable/reasonable way to go about searching for unknown proteins in conditioned medium. I have cultures of cells and need to look at soluble proteins released from these cells. I'm looking for relatively small proteins. I'm starting from scratch.

Hey guys, I was wondering if anybody knew an acronym for glycolysis intermediates or glycolysis enzymes? My test is coming up in three days so I NEED SOME SERIOUS HELP

How much energy do we get from each phase of oxidizing a triglycride molecule with 12c chains of fatty acids. I tried calculating it but i get numbers not in the range that i think is right. --------- -so Glycerol goes into glycolysis and make 1 nadh and 1 atp? -in the intermediate step pyruvic acid is made while forming 1 nadh? -in beta oxidation i know that 2 carbons are broken down each time but iam not sure where to go from there thanks

This is the earliest description of de novo production of authentic oxytocin in Leydig cells that I can find in the scientific literature: Mechanisms by which hypoxia augments Leydig cell viability and differentiated cell function in vitro (PhD Dissertation, 1993) by Mark A. Kukucka, MS, DVM, PhD Department of Biomedical Sciences Virginia-Maryland Regional College of Veterinary Medicine Virginia Polytechnic Institute & State University Blacksburg, Virginia 24061-0442 ABSTRACT: The 1980s heralded the discovery and identification of extra-pituitary sources of the neurohypophysial hormone oxytocin in non-neural tissues of several anima…

Hi! First I apologize for my english. I electroporated a vector (containing 3 repeated regions of 250bp) in bacteria DH5a. I think that normally, DH5a are known to have a mutation in RecA that permit to avoid rearrangement of the plasmid. But the bacteria recombine the repeated regions of my plasmid and I obtain a plasmid of abberant size. So I search a strain that do not cause rearrangements or deletions within plasmid DNA. Can someboby help me? Thanks a lot Have a good day

Hello everyone, I am new here. I have a question about this Biochemistry: See # 16 on this link (http://www.ncbi.nlm.nih.gov/books/bv.fcgi?rid=stryer.section.556#575 ) and the answer can be found when you click on "answers". When I did it, the sequence came out as (AV)®(SY)®. Under the Trypsin, it said it cuts on the Carboxyl side, so I put R-SY but in the answer its the other way around ...®-(YS)... Can someone explain this? Thank you.

Just a quick question for the practising biologists on the forum. I've just started a research project and will be performing some nested PCR on a 4kb sequence that will be genotyped in a number of individuals (~50 initially). Does anyone know, off the top of their heads, of a high fidelity polymerase that is capable of performing long PCR's? I'm going to be testing some pre-designed primers and such, and all the other reagents and thermal profile are going to be tested empirically so they aren't an issue right now. Cheers in advance.

Hi.... I’m just thinking that if atoms arrange themselves with each other by exchanging electrons (no?).... Then what makes them distinguish between each other? Meaning why don’t the atoms of my hand (whatever they may accumulatively be) mix with the atoms of a wall, as I lean up against it? Any suggestion, comments and opinions are appreciated. Thank you in advance....

Do you think physical chemistry is important to molecular biology? I think organic chemistry and biochemistry is important for such but I am thinking physical chemistry would also be helpful. Would that be enough education in say chemistry to study molecular issues in the life sciences like microbe ecology? Such as if you wanted to be able to independently study bioremediation via microbe behavior what education in chemistry would be sufficient?

hi i m studing "Exploring Genomes and Proteomes in practice" and its written that u can disrput the cell membrane either by repeated freeze-thaw cycles, osmotic shock, grinding or homogenization. The resulting homogenate is fractionated (separated into individual fractions) by centrifugation, chromatography or electrophoresis. so can someone explain it to me in brief