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Auréliedep

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  1. Hi! First I apologize for my english. I electroporated a vector (containing 3 repeated regions of 250bp) in bacteria DH5a. I think that normally, DH5a are known to have a mutation in RecA that permit to avoid rearrangement of the plasmid. But the bacteria recombine the repeated regions of my plasmid and I obtain a plasmid of abberant size. So I search a strain that do not cause rearrangements or deletions within plasmid DNA. Can someboby help me? Thanks a lot Have a good day
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