Biochemistry and Molecular Biology

I am a Textiles design student and i am trying to make plants have more involvement on everday life. I want to create a way of growing plants and vegetables in a clear solution so you can see the growth process i have no idea if that is possible?! i have looked into Perlite and even tryed adding plant food to water storing crystals to see what would happen but i think it would just harbor root rot in the plants. If i can't work something out i will have to turn to real hydroponics!! Also what are the benefits of growing with agar?!

Hello, this is my first post! I'm struggling slightly with predicting the km of my reaction,from the reaction mechanism, i'd just like to see which of two km values i've ascertained by two different practical methods is likely to be more accurate. I'm working on a system which consists of a reductive and oxidative half reaction in a standard ping pong mechanism. I have conducted pre-steady state and steady-state assays to ascertain all the relevent kinetic parameters. I'm currently trying to see if km values predicted by the mechanism match those determined experimentally, I know the kd values and klim values but the mechanism i'm using states: km of oxidising substra…

If you where to inject Ion (oxygen) (negative charged) into the human body more specificity body cells would this cause cellular processes to shut down such as the electron transport chain in respiration. Which ones , and how long.

What is the differences between: Elements Compounds Isotopes and Radioisotopes?

I'm looking at the 12 GLUT transporters and the mechanisms of action for each. I've found that GLUT 1 & 2 are passive transporters and I was wondering if all 12 are passive transporters or if there's a different mechanism for vital areas in the body (like the brain, testis, kidney). I've found that GLUT 3,5,6,8,9, and 10 receptors are found on these vital tissues, do they perform passive transportation also? I'm using Lehninger's Principles of BioChm as a reference. Any help would be much appreciated. Thanks. -Links

I am working on homework and i had a question about the TIM barrel which i will post here: The high-resolution crystal structure of TIM - posphoglycohydroxamate complex revealed a 10 residue loop, which closes over the enzyme active site in the TIM barrel once the posphoglycohydroxamate binds to the active site. posphoglycohydroxamate binds tightly to TIM. Explain why. In the enzyme alone structure, the loop is flipped up like a hinged lid. A deletion of this loop has no effect on the integrity of the TIM fold. However, a small molecule of methylglyoxal (CH3-CO-CH=O) is detected as a product. Provide an explanation for the formation of methyglyoxal by the mutant TI…

Assume I must do microarray for the first time. How long would ti take me to obtain good results? Take into account the time it will take me to master the technique.

I am a student of Molecular Biosciences (third year) and for a year I would be interested in this theme related to biochemistry. Unfortunately I can not find a text that adequately supports me. I'm studying "Essential of Computational Chemistry" - Cramer, but for my current situation, perhaps, is too complex. Do you know any more introductory text? Thank you! PS: sorry for my English, I'm italian and I're not yet able to write (unfortunately)

Hi.. I'm just wondering if anyone could help me with how would you generate a sequence for a plasmid that has been digested using Xhol/EcoRI Thanks in advance

Hi peps, I'm in dire need of question banks of 'molecular_biology_concepts_and_experiments_karp'. Can sombody please send me this question bank.

thanks

Hi all! I graduated 2+ years ago with a degree in biochemistry but have been out of the field since then, working retail as it was all that was available. There are now several job opening I am considering but after my first interview I feel out of the loop on much of the current science and research technology that is out there. Can anyone point me to a few good biochemistry or protein chemistry text books that would catch me back up? Thanks so much!

Hello Folks. I am a non-scientist with a few scientific questions, which I'm submitting here in hopes of an answer or two from you, the experts. I have purchased some used property through a normal, straight-forward business transaction, through a seemingly reliable source. As it turned out, this location was contaminated with something that, when damp, has made me ill, and when dry, has become very irritating when breathing, and on the skin. I normally wouldn't post on this, but it has been an ongoing situation for some months now and has been difficult to mitigate. I have tried bleach (didn't work), 35% H2O2 (worked but corrosive/toxic), 91% alcohol (works, but…

Hi, I searched for a while in Google couple months ago, but what website/link do you recommend for detailed processes for photosynthesis and cellular respiration? I had one year of college biology, but I know that what I learned for photosynthesis and cellular respiration are very condensed than what they are (I mean, we mentioned PFK and other enzymes, but still...). Do you have any good site for demonstration? Thank you~

I just have a simple question that I would appreciate any help with: Concerning transcription with eukaryotic RNA polymerase, what type of assay may be used to determine if promoter opening has occurred (i.e., distinguish between a closed and open promoter complex)?

I am confused about the principle of Ki. I understand that Ki represents the affinity of the enzyme for the inhibitor, with a large Ki showing a strong inhibitor. In competitive inhibition, Ki serves to double the slope, while in noncompetitive inhibition Ki serves to inhibit the enzymes by 50%, but what does all of this mean in terms of applying it to a problem. I understand there there is no formula for Ki, like there is for Km, expect [E]/[EI]. What is the relationship between Ki and Km and can a given Ki value describe the Km magnitude if it is not given? Thanks and sorry for the contorted wording of my question.

Arsenic is structurally similar to a phosphate group therefore many enzymes that require phosphate will also utilize arsenate. Predict the net reaction catalyzed by PGAL dehydrogenase if phosphate is replaced by arsenate. I dont know what i should write for this question however i did try: Arsenate structure is similar to the structure of a phosphate group therefore in Glycolysis, Arsenate can easily replace the inorganic phosphate that produces 1, 3 bisphosphoglycerate (BPG) therefore yielding 1-arseno-3-phosphoglycerate instead. The BPG molecule is expected to produce an ATP molecule by phosphorylating ADP to ATP (giving away phosphate to ADP) but because BPG i…

I am considering modified form of Michaelis-Menton equation specially concerning non-competitive inhibition. The picture attached described what I have done... But why must K3 equal K4, I know it is the experimental result, but is there any actual reason can account for this?

Hello everybody! I want to know if there is any relationship between Biochemistry and the feelings of Love for the opposite sex?

Who know at what temperature the viruses start to denaturate? and why when you use the autoclave, you always rise the temperature to 120 centigrades and no more? thanks.

What are the most important differences between the two?

When DNA is replicating, how can the polymerase add so many bases so fast? Where does she get them?

I've question in molecular .. Could anyone explain to me the idea of " Mapping the nucleus S1" for determining the initiation site at 5' end ??!!! Thank you

Pure red cell aplasia is said to be an autoimmune reaction. How can an autoimmune reaction cause the bone marrow to cease producing red blood cells?

1) hexose isomerase reaction is endergonic reaction G6P <-----> F6P deltaG= +1.7kJ/mole how does this reaction proceed in the forward dirction during glycolysis ---- im not sure but is it because the proceeding reactions are exergonic which would push this reaction forward 2) the body stores glycogen (a polymer of glucose)as emergency store for energy generation. Explain why we dont store a) glucose b) ATP c) fat as such emergency stock last thing can someone please explain the futile cycle for phosphofructokinase frutose 1,6 biphosphate thank you soo much in advance