Biochemistry and Molecular Biology

Can bacteria feed the world soon, and become 10,000 times more abundant than agriculture, enabling vertical farming that GROWS IN THE DARK? TED TALK: https://www.youtube.com/watch?v=mQVdJjToMYk June 2016 business article: http://www.launch.org/about/news/kiverdi-is-turning-carbon-dioxide-into-food-for-the-world I've also seen TED Talks that claim algae could end up being 1000 times more land efficient than current agriculture, and that's if we learn to eat it directly ourselves, possibly 3d printing it into artificial meat? Anyone know if there is progress in these areas? But there's also algae to food via fish, which is already happening in Australia: …

Say it's the apocalypse, and we need fresh drinking water. Silver is known for its anti-bacterial properties. Would adding a whole silver dollar, to say 1 liter of water able to kill bacteria in it? Just a curious question. ~EE

Hi, I'm a newbie in biology with interest in genome engineering and I wonder if you could help me with a doubt, it may sound silly but I need to be sure. If I introduce a lentiviral CRISPR/Cas9 system for gene silencing into a population of different types of cells, the system will only produce the silencing of genes in the cells containing the gene? What changes could produce the lentiviral CRISPR/Cas9 system in other types of cells?

A pre-RNA world #Genetic_code with only a single (but reversible) nucleobase? Today there are four different types of #nucleobases which stack on top of one another like plates to form the RNA molecule Perhaps in the original #PAH world there was only one type of #nucleobase but it could be put on the liquid crystal stack in one of two different orientations by simply flipping it over like a pancake. http://en.wikipedia.org/wiki/PAH_world_hypothesis #Polycyclic_aromatic_hydrocarbons are the most common and abundant of the known polyatomic molecules in the visible universe, and are considered a likely constituent of the primordial sea. #PAHs , along with fullerenes (or "b…

I dont get it? They say virus and bacteria grow in the body when its acid, sugar etc can feed the virus and the body becomes more acidic. But its stated that absorbic acid and vinegar etc kill viruses due to the acid. Can someone explain this to a non biochemistry guy thanks

Hello all! I am trying to work out if I can predict if a sequence is going to be difficult to grow in a culture. I know that things such as size (like in the muscular dystrophy gene) and secondary structures (like ITRs in AAV) famously make gene sequences very hard to yield in culture. Are there a number of elements I should look for that will help me predict if something will be difficult? Are there other examples of very difficult genes you can think of? Maybe G-protein coupled receptors? Do we know why these are so difficult, and what they do to the cells during culture? Thanks!

Hi, everyone!! Recently an interesting study has reported. Zhou et al., 2016. J. Cell Biol. http://dx.doi.org/10.1083/jcb.201605101 According to this study, enhancing anterograde axon transport of mitochondria is essential for regeneration of injured axons. Neurons need a large amount of energy provided by ATP to extend their exons when they are injured. Mitochondria produces ATP essential for neuron growth, survival and regeneration. This study shows that in mature neurons, the motility of mitochondria is reduced and the reduced mitochondrial motility might be the cause of the deficit in regrowth capacity of mature neurons. Moreover, this study found …

My Biochemistry teacher said it was a sp2d hybridization, then I read somewhere it was a sp2d3 hybridization instead. I know there are six coordination bonds, but which kinds of orbitals are part of those bonds? Also, how does the hybridization take place in this case? I'm very confused. If anyone could clarify this, I'd be very grateful. Thanks.

Does anyone know of a computer program that allows you to see the 3D structure of a protein, and all the side chain interactions? No urgent reason. I'd just like to have one ~EE

Hello! We analyse urine and we cout cfu number. Is that what we count number of bacteries (particular bacteries) or clums of bacteries (colonies)? So, if these are colonies,, then itis significant,when we have more then 100 000 COLONIES i 1 ml, and that is MORE then just 100 000 bacteries, isn't it? MANY THANKS FOR HELP!!!

Hi all, let me warn you in advance, this is a very speculative question ;-) For my master course, I am asked to develop a new research proposal in the field of synthetic biology. The topic I was interested in is the dilemma that Vitamin D synthesis in animal needs high energetic UV radiation which is harmful for DNA and RNA and yields are very sensitive to physiological aspects (melanin pigmentation) and sufficient amount of UV radiation (time exposure,latitude etc.). So my idea was to create an artificial system, which uses "normal" visible radiation to synthesize Vitamin D: The synthesis of Vitamin D occurs in the our skin tissue, where the molecule 7-dehydroch…

Hi! I have a problem with a fused protein. I want to know the optimal pH and temperature range (among other physical properties) of a fused protein, made of two mini-proteins. However, I'm still waiting for my DNA synthesis to arrive in order to produce it and I only have a few properties of the mini-proteins. I do have my fused protein modelling and structure, but I cannot predict any of these parameters. Does anyone know about how can I predict (in the most accurate possible way) pH, temperature and isoelectric point of my fused protein without experimenting? Thank you!

I'm looking for the right term to Google with. What is the term used to describe the minimum number of copies of a pathogen required to cause infection?

Hi everyone..how do we make a lipase standard curve of reaction rate vs time? I have 4-nitrophenyl decanoate as my substrate and other buffer and solution such as Tris-HCl, sodium taurocholate and NaCl.

Hello Why the plant genomic DNA migrate as a single band in agarose gel and what type of DNA is? (circular, linear, supercoiled,...) Thanks very much

Hello all! I am an undergraduate working on a project where we are trying to extract collagen from the fat pad of a human (in the heel). We attempted to extract collagen from fresh tissue samples of a human heel pad using the protocol from Pacak, MacKay, and Cowan (2014, Jove). Then we attempted to electrophoresis the sample in a 7.5% SDS-PAGE gel along with a protein ladder and a sample of standard collagen from Sigma Aldrich (5 mg/mL). When we developed the gel (staining overnight and destaining overnight), all that we saw run was the ladder and the standard from Sigma Aldrich. I am very confused on what might have happened since we followed the procedure exactly an…

Hey. I'm looking for an inexpensive process to synthesize a sturdy material from bacteria, plants or fungi. What species do I need? How do I extract the compound from the rest of the organic matter? Does the compound need to be heated or treated in any way? Thanks

Hey guys so im looking into buying my first microscope and looking to spend between £100 - £200 i know its not much but for someone who wants a nice hobby looking at pond life, taking swabs of the cheek, blood samples i understand a compound microscope to be best for these i have come across 2 names in the microscope world but i don't know whos better apex microscopes amscope microscope. i know there is also olyompous but addons are very expensive

Ive gold panned a few times and the reason I thought gold was so hard to find because it could only be made in an extremely hot burning star that is even hotter than our sun. Now I search on google and a few years ago reaserchers at Michigan state figure out another way gold is made. The say there is a bacteria that lives in extreme conditions and this bacteria produces gold from the ions it eats. Now to me this seems bogus. Because how can you create a basic element from just a biological organism. Perhaps these bacteria just live in an high gold concentrate area and since gold is a very durable metal it is the only thing these bacteria do not digest. Wha…

I just wonder, does anyone know of a handbook similar to Marri, Grenner (auth.) Human biochemistry. I admire its clear and concise style, but it seems to be a bit outdated, since it was out in 1993. To put it otherwise, I look for a comprehensive but not redundant general handbook on human biochemistry with a particular emphasise on lucid and clear style. Can anyone help?

Hey everyone. I found that some people here are pretty familiar with the surface plasmon resonance concept so I thought of sharing one of my favourite tips - preconcentration! This technique basically allows researchers to use even less ligand when conducting SPR experiments. Since expressing and purifying proteins is time-consuming and purchasing proteins can get expensive, saving as much ligand as you can is always ideal. Even a lot of our own users were asking us to explain preconcentration, so we mput together a blog post to help out. Hope you enjoy You can visit the blog at: wedon'tallowadvertising.com For those of you who'd like to know more about surf…

16/07/2015 by NovoPro Introduction: One kind of the venom from Queensland cone snail hidden thousands of peptide toxins, which were potentially contain the value of the precious drugs, possibly provide new leads of pain and cancer drugs. In recent PNAS study, the researchers described a new method to analyze venom toxin structure. Paul Alewood is the University of Queensland professor of molecular biology, he said the study is the first to give a present in a single cone snail venom toxins. This cone snail research called conus episcopatus, living on Australia's east coast, it is one of the 700 kinds of different cone snail. Cone snail venom mixed with many complex c…

We know that living creatures are biochemical machines. Well I want to question you something: Could it be that many diseases are very complex in their origine. I mean there has not to be a genetic cause for a certain disease. It's more complex? This also means that if you take the diseased tissue and make epigenetic, transcriptome and proteome analysis, you would see that biological the tissue is out of order? But on the same time there is no direct genetic cause, no evironmental cause? The cause it not biochemically understable for humans, because it just happen that organs run out of order biochemically? Biochemistry is too complex?

Bachelor degree. Can't upload word doc. with results and intervention/set up? But graphs shows mRNA content in the liver from muscle specific PGC1-a knockout mice (MKO) and littermate lox/lox control mice (LOX/LOX) in the fed (FED) and 24h fasted (FAST) state. I need suggestings/help with: 1) Upregulating of PEPCK mRNA after fasting Possibilities?: - The observed increase in PEPCK mRNA content in both lox/lox and PGC-1a MKO mice after 24h of fasting demonstrates, that PGC-1a is not required for this response. - The higher PEPCK mRNA content in PGC-1a MKO mice in the fasting study, may indicate that lack of PGC-1a leads to compensatory increase in t…

Hello, I cannot find any information/ solid article whether enzymes contained in the food and pills that have been taken perorally can pass through the stomach or intestine cells into the blood circulation. I am mostly interested in transport of hydrolases contained in food/ pills. Can they pass intact through the cells lining digestive system to blood? What are the mechanisms? Thanks!