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fredreload

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Everything posted by fredreload

  1. I still sounds kind of harsh, but you need to be baptized by science :D, jk. Chromosomes make us equal, jk
  2. Well, let me enlighten you, this is the type of work the geneticist are doing right now. Combining and manipulating the yeast chromosome. Well alright, I am not sure if they are doing this in the nucleus or plasmid, but it seems pretty likely to be in the nucleus concerning the use of crispr cas9. Size has never been a problem, for the DNA size, you could chop chromosome into 1000 1 million pieces DNA, the end result is you can still combine it inside the nucleus like a transformer, or with ligase. Here is how John Hopkins build a synthetic yeast genome from scratch. This is how an Australian University destroy one of the chromosome with crispr. Now if you've read all three of the articles from the links I posted you'd be wondering hmm, this is the time to do some serious work on chromosome manipulation. We could create an artificial chromosome, we could combine chromosomes together, and we could delete chromosome. Maybe we could smuggle chromosomes in and out of the cell(nucleus) when needed. And I haven't got into regeneration research, people are also getting close on that field, check Michael Levin's newest research on regeneration.
  3. My mistake, I think you need to become the matter wave for this to work. But at the subatomic core they are all the same. I can't even figure out unit space don't ask me on this one
  4. A subatomic molecule is a portal. Because there are similar subatomic moleules making all of them connected. One indistinguishable from the other. For instance if you got two identical cups on the table, you dunno which one is your cup, and which one is my cup. But you cannot go through a subatomic particle, because it is too small, and it has an electric field. So you can condense it into a matter wave like Bose Einstein condensate and drop something through it?
  5. I think I was being harsh, sorry John Cuthber. I'm not a chemistry expert =/, just a bypasser
  6. Well, alright, it's good that you got questions, because there is some that I like to "get" an answer myself if you happen to "know" it. 1. Yes 1 million base pairs are too large for a virus, so I suggested using a bateria instead, I dunno if 150 million base pairs(the size of the X chromosome) would fit inside a bacteria. 2. Yes there is currently no method of bacteria targeting the entire body, so I made a separate post hoping that some wound repair mechanism would target every cells in the body(still working on this). 3. Once the 150 million base pairs of DNA from the bacteria "enters" the cell. The DNA goes into the nucleus, that's what virus do, all DNA goes to the nucleus for processing if not integrated into the host genome. From there the proteins in the nucleus would assemble the DNA into a chromosome(if prebuilt correctly, I can't find any articles on chromosome "building" not "packaging"). 4. Once you get the X chromosome in the body. It's like having a bear chromosomes in the cell but you do not turn into a bear. You need to activate them through proliferation by regeneration(you can check my posts on regeneration). 5. Destroying the chomosome is an article I found done by Austrlia institution using crispr cas9. P.S. Please take the time to read my work before you completely reject everything and say that my idea is doomed to fail. I'd like to make some friends on this forum.
  7. It's alright Charon, Fred just needs a break from science.....forum......lates
  8. Hmm, I wouldn't know what I would look like with 2 X chromosomes. I might be one of the top female waitress
  9. Need some work on the whole body delivery system. I don't think that would be easy task
  10. 1. Hey Charon, what are the currently available methods for signaling molecules or crispr cas9 delivery into the entire body? What percentage of the body is covered using this method?
  11. Hmm, the inserted X chromosome may need to be set to the correct gene expression and epigenetic changes. For setting the gene expression on the X chromosome, a patterning signaling factor may be needed. As for epigenetic, I got no idea
  12. Hmm, you'll need a bacteria to smuggle the amount of DNA of this size. 150 million base pairs. Oh yeah
  13. Well, the DNA strand would get turned into a chromosome inside the nucleus, if it is made correctly = =, now this part is speculation. I know there are chromosome repair mechanism, but I dunno how it factor into this. The university of John Hopikins have successfully built an artificial chromosome on scratch, they know how to integrate it with histones and other thing for yeast chromosome
  14. I've prove that transdifferentiation is possible, check my other post on transdifferentiation. This is a scientific speculation of how it should work.
  15. 1. Pack a pre-built copy of your X chromosome's DNA into a virus. It is a strand of DNA with 155 million base pairs that hopefully would fit through the nucleus pore. A chromosome cannot fit through the nucleus pore, so we send it in as a strand of DNA and have it built into an X chromosome inside the nucleus. 2. Make sure each cell in your body receive a copy of the virus package. I dunno how to get this to work, each cell only needs one copy of the X chromosome, having more than one would be troublesome. The epigenetic tags on the X chromosome might also need to be different for each cell, that I have not consider. The packaged DNA would enter the nucleus through the nucleus pore and gets integrated into an X chromosome. 3. Now you have a XXY chromosomes in each cell, destroy the Y chromosome by sending in crispr cas9 enzyme to target the Y chromosome. The crispr cas9 enzyme would target the centromere of the Y chromosome and attempt to destroy it. 4. Now every cell has a XX chromosome, initiates regeneration on the entire body for patterning and structural formation. The regeneration technique is induced without scar formation. When the patterning signaling factor is added in it would attempt to restructure the whole body based on the chromosome.
  16. Alright, so based on idea of smuggling and inflating a chromosome into a nucleus. I will have to prove that transdifferentiation is possible. And I speculate that dedifferentiation is a form of transdifferentiation. The way that somatic cells becomes induced pluripotent stem cell is a form of cellular transdifferentiation, and therefore all method of transdifferentiation is possible. Let it be liver cell to neuronal cell or muscle cell. I speculate that it should be possible for direct conversion.
  17. Essentially all the chromosomes need to exist inside the nucleus, not outside. It's like football and you need to get it into the nucleus to touch down. So transformer wise, we smuggle DNA sequence in chunks(like messenger RNA) through the nucleus pore into the nucleus, and we assemble an entire chromosome inside the nucleus with DNA ligase. What I want to do with Crispr Cas9 is to destroy the original chromosome else having 3 copies of a chromosome could be a problem. P.S. Like 2 million pieces of DNA, assemble by hand/automatic
  18. According to Wikipedia on scar. "The scarring is created by fibroblast proliferation,[25] a process that begins with a reaction to the clot." Fibroblast is a biological cell according to Wikipedia. Liver also has immune pathogens from blood so I am ruling that out.
  19. Alright, improved version. 1. Stays 2. Forget about mounting a laser on a machine and put it into the body. This could work but not in the next one hundred years. God knows how it will move and cut DNA at the correct place. The answer, use the enzymes that is already in the body. Helicase, DNA polymerase, and other enzymes are working at, if I must say, 100% perfectly to keep our body function readily. So any improvements will be an add on to the already existing enzyme. And there is Crispr, I am not taking credits for this, and it is not working 100% of the time until it could be adapted for human use.
  20. Alright, forget bout crispr, there is never going to be a time where crispr can modify every single parts of the body and at a precise location. That gives two methods which is currently science fiction based. 1. Modify DNA with optogenetics. Simple as that, we got the light tweezer, we target a cell, and we use light to "cut" and "piece" together DNA to the desire strands we want. We either use one laser to modify every single cells in a fast pace, or have multiple beams that modify all cells at once. In other words it won't drift too far apart from magnetic field control. 2. Modify DNA with nanomachines Well, talk about advancement in machinery in the nanometers. This programmed robot is precise in such a way that it works 100% of the time and is error proofed. While having a wireless connection with the outside computer. Talk about coordinated controlled drones. I want you to tell me which method is more likely to be done, and in how many years.
  21. Apparently it's something that the adult stem cells lack and embryonic stem cell has. Summary: 1. Both adult stem cells and embryonic stem cells proliferate to differentiate new tissue. 2. Adult stem cells sometimes produce scar, also know as fibrous cells/fibrous tissue. 3. Adult stem cells create compensatory growth, embryonic stem cells completely regenerate.
  22. So you need another patterning molecule to formulate the liver as it is regenerating, even adding that molecule alone to the compensatory organ should cause it to reform given that it already has the regenerating ability. I've been searching for that molecule and as far as I know it is called Sonic Hedgehog from the hedgehog pathway. But with the liver case it seems to be something presented only in the embryonic state
  23. Alright so, both hydra and starfish are capable of complete regeneration. But what would happen if stitch half of hydra and half of starfish together. Here is the below speculation. 1. The hydra and starfish cells fight to the death, each trying to take over the other in cell signaling war and immune response war. 2. The hydra and starfish grows into separate beings without fighting each other, hydra and starfish. 3. Also if you put human cells next to it would it attempt to take over the human cells?
  24. After studying stem cells usage on heart tissue repair I found this message. The stem cells do not turn into heart cells themselves, but their growth factor help the heart cells to proliferate into new heart cells and clearing away scars. This means if I inject patient A's stem cells into patient B, I do not start growing patient A's cells in my body. Is this correct?
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