Biochemistry and Molecular Biology

I have a question, How come if we immunoprecipitate with monoclonal antibody, then we should blot with polyclonal antibody, or the other way around? Thx ~

Hey everyone. I'm starting an internship in a few weeks and I'm a little confused by one aspect of what I'll be doing. I don't know how to prepare (stock) solutions, such as 28% NaCl. (Actually I'm not even sure that's the correct way to note that.) Can anyone tell me how to prepare solutions or direct me to a website? Thanks. Helix

Found some oriental poppies (Papaver orientale) in my garden, i wonder, how is their alkaloid content compared to the opium poppy? Do they still contain morphine and codeine in lesser amounts or do they lack of it completely? No worries, im not gonna make opium, i have botanical interests and plants secondary metabolites as means of defence interest me.

Hello everyone. I'm reading a published scientific paper (trying to start interning at the biodesign institute at my college and this is some "homework" i got), and have no idea what those 2 terms mean. It's used in many places throughout. EDIT: okay, I think more info is needed. The paper can be read here. It's not long. It's a study about human rhodopsin (visual pigment) mutations which lead to retinal diseases. Here's a couple examples of sentences using the phrase: "For a given amino acid site, the average chemical severity of interspecific substitutions is a simple average of the severity of all ancestor-descendent amino acid differences throughout the tree." …

Hello!! Right we have a leetle problem in our lab and I was wondering if any wise folks out there may have an idea or solution...any thoughts at all most welcome.... Something is killing our E.coli...about 2 hours after inoculation the E.coli start to lyse. Initially this happened infrequently so it wasn't really a cause for concern, but now it happens almost every prep. Sometimes 1 or 2 flasks will survive, so I think can safely rule out problems with reagents. We have tried using new flasks, new stocks of antibiotic, new medium, transformants etc etc. I know that this sounds like a phage infection, serial dilutions of the lysed culture onto plates of confluent E…

Can any one tell me what is death? According to my knowledge a person is called dead when all his brain cells(neurons) are dead. If that is the case then if we are able to bring the neurons to the previous(alive) state then that person can be raised from death? Someone please clarify...

Someone please define (in Undergraduate-level terminology) what "Synthetic Biology" is. How does it differ from regular Molecular Biology? Mike

I'm new here and I have no idea if this is the correct forum to post this thread on or not, but here it is. The first question: How do I clone a novel gene that encodes a protein that has sufficient evidence to support its existence? Since I don’t want to screen libraries, my first thought is this: 1) Compile sequence alignment blocks of known homologous proteins. 2) BLAST against whole genome of chosen animal or trace archive. 3) Build a contig and use it to design GSPs. 4) Test the GSPs with genomic template for animal of choice. 5) Go into 5’ and 3’ RACE. The second question: If I decide to go with my first thought, how do I do parts 1 and 2? I suc…

Hi all, I'm currently writing up one of the experiments for my course but there is something that has me stumped where to find the info on it. Part of it requires me to find out the molecular weights of myoglobin, carbonic anhydrase, BSA, ovalbumin and gamma globulin. I have some really rough figures for those, but the problem is that I can't find anything reliable on the internet (most of it seems to have been written by someone off of the top of their head, no two figures seem to add up) and the resources I have at my college are..limited at best. I was hoping someone might be able to point me in the direction of a book or a website that has information on…

I read these two things in the book "Molecular Biology of THE CELL" (fourth edition): 1. (Page 540) "In mammals, for example, linear DNA fragments introduced into cells are rapidly ligated end-to-end by intracellular enzymes to form long tandem arrays, which usually become integrated into a chromosome at an apparently random site". 2. (Page 541) "If a DNA molecule carrying a mutated mouse gene is transferred into a mouse cell, it usually inserts into the chromosomes at random, but about once in a thousand times, it replaces one of the two copies of the normal gene by homologous recombination". Now, I would like to know if what said in #2, that the DNA molecule…

Does anyone know the composition of the TM-1 medium by Bagga et al. 1992? This is usually used for alfalfa (Medicago sativa) regeneration. Tks

Apparently, for dna synthesis between circular dna and linear dna, T4 phage gp43 Pol allows for dna synthesis to commence WITHOUT prior addition of a primer, whereas the primer is required for circular dna. As a comparison, a test run with DNA Pol III demonstrated that the primer is required in both cases for that enzyme. In a kinetic plot of linear dna synthesis with and without a primer, it is evident that synthesis with primer is faster, while synthesis without the primer has an initial retarded period, followed by a slower rate of synthesis. Is there some kind of physiological/structural difference going from circular to linear dna that allows gp-43 Pol. to synth…

Is germline gene therapy the same as gene therapy? What are advantages and disadvantages? And what is the history of germline gene therapy? Could you please give us website Thankyou

For proteins, living systems are specific for L-AAs but for carbohydrates that degree of specificity is not observed...how do we explain it in the context of evolution..? Also please tell me what is the degree of specificity for D-sugars & L-AAs...i mean when the specificity can be violated naturally, i'm interested in knowing that... I got that D-Alanine is a bacterial marker [ i guess due to its role in murein formation of bacterial cell wall] but i don't know if any 'polypeps' contain D-AAs , & if yes ...upto what extent & all... Similar querries for L glucose...& alph & beta sugars... Please help me... i'm searching the web...sugge…

Need serious help: Can DNA adducts be measured quantitatively by HPLC-UV alone? (Aflatoxin-DNA adducts in particular) What are other methods that I could use in measuring Aflatoxin-DNA adducts that are not so expensive yet will yield reliable results? Thanks:-)

How can I explain the genetic basis of a disorder that occurs with equal frequency among chicks and dudes and that doesn't follow basic patterns of Mendel inheritance??????

My 11 month old daughter has now got her 1`st Tooth, naturaly she`ll need to start brushing her teeth soon and will need a brush And toothpaste. I said to my wife I don`t want anything with Fluoride in it, now I`m not entirely sure Why I said that, I just don`t like the idea of her being exposed to such things just yet (some inevitably gets swallowed). is my "Gut reaction" justified or am I being too over protective? we have a free sample of baby toothpaste here and it contains 500ppm of Fluoride, I want to throw it away and get some Without Fluorine. anyone had experience with this sort of thing or have any evidence eitherway? Appreciated

hi everyone am currently wrighting my bacholorate dissertation, and was wondering if i could pick your brains on a subject? basiccally, i was wondering if anyone knows whether the prokaryotic genes encoding restriction enzymes will be properly expressed by eukaryotes, in particular humans i cannot find any published data on this subject, so if anyone has any idea -- anecdotal or otherwize -- i would very much appreciate it thanx all

http://www.ess.ucla.edu/huge/report.html#s

From: http://math.ucr.edu/home/baez/subcellular.html Ok this is some spooky shat. This means that retroviruses are basically organisms that can survive by integrating themselves into a hosts DNA. This means that the virus can basically be passed down to your ancestors. So if your great great grandfather was exposed to “human immunodeficiency virus 1” some point in his life, you or your kids could also be infected with the same virus (which I say again is basically a parasite and a separate organism then yourself).

John Baez has an interesting webpage about them http://math.ucr.edu/home/baez/subcellular.html This includes things much smaller than usual virus including Spiegelman's Monster which is only a couple of hundred base units long. At the other extreme there is this virus which is bigger than a lot of bacteria. So big that for a while it was not even recognized as a virus. http://www.livescience.com/animalworld/041111_giant_virus.html

i havent been on these forums since 04, i was just wondering if theres any new info on this subject last i heard they could freeze but not thaw, did we make any progress?? is it impossible?? sorry if this question was asked recently and if you guys dont feel like talking about it just give me some links and i can read up on it, thanks

I am having problems understanding this subject and i wanted to know why plants need to be able to produce ATP by respiration AS well as by photosynthesis. I know its got something to do with using the co2 produced in light reaction ie photosynthesis to create Atp in dark reactions respiration....... i think. Help!!

What is it made out of on a molecular level. I know it's not a muscle so what is it?

When you exercise, you lose weight. Which is why fat people are often encouraged to go running to lose the flab. But what is the biochemical process that occurs here? My guess, and please correct me if I'm wrong, is that when you exercise your muscles turn Adenosine triphosphate (ATP) into Adensosine diphosphate (ADP) in turn releasing energy to propel you along. To complete this "krebs cycle", as I believe it is called, the body metabolises sugar in the bloodstream to convert ADP back into ATP so that the proportions of these two chemicals don't become too uneven. Then when there is a lack of sugar, the body starts using fat to create ATP, thus burning it. So the q…