snowysummer

thx a lot~

I have a question, How come if we immunoprecipitate with monoclonal antibody, then we should blot with polyclonal antibody, or the other way around? Thx ~

thank u for ur reply~ DNA was isolated from salmon testes. TES was added when the testes was ground up into a paste, and centrifuged. after pronase digestion and addition of sds, dna was precipitated in ethanol in the presence of sodium acetate. Then DNA was redissolved in TE buffer.

can anyone help me with the following question? i don't understand how come before DNA is isolated, TES buffer is used, but after DNA is isolated, TE buffer is used. The only difference between the two buffers is the presence of 0.15 M NaCl. How come NaCl is required before DNA is isolated? Thanks !!

As periodate may cut glucose into formic acids, how many cuts take place on a ring structure of glucose, and how many on the linear form of glucose? thank u for ur help!

thx a lot!! i thought it's a way to study proteins and nucleic acids. ....

know it's a really basic question...but are crystallography and x-ray diffraction the same thing?

primary standard is a substance that has high purity. Dissolve the standard into solution, then titrate it against a sample to determine the sample's concentration.

Can anybody tell me what primary standard is usually used to standardize NaCl?