Biochemistry and Molecular Biology

Hi, I have been trying to find away of detecting if knot weed actually dead or just dormant. I have considered detecting for the presence or absence of mRNA (using the poly(A) tail) the idea being that mRNA will only be transcribed if the cells are still alive and functional. What do you think, do you think this is plausible or should I try to come up with something different Thanks, Erin

By this pill I mean creating a chemical solution which would accelerate the processes our body undergoes while we are sleeping 30min of speed sleep would equal lets say 8 hours of normal sleep time is money, so if we could reduce our sleeping time without losing the benefits, it can be a life changing invention.. is it possible? and if it is, why can't we find it on the market?

Bioinformatics is giving me a headache! Lots of authors mention the terms 'protein fold', 'protein superfold', 'protein family', 'protein superfamily' and 'protein domain' interchangeably. How do you guys use these terms? What's standard in the protein community?

I recently googled "AIDS" and "Cancer" and stumbled across a site that was dated 2005 which stated that a common ancestor billions of year ago allowed the DNA in viruses to evolve and that it uses the the same basic protein "fold" to construct the critical outer shell. I question this common ancestor theory insofar that billions of years ago the existing forces at that time are the same kind of forces we experience today. A sphere is formed because of these forces . Would it be more acceptable to saythat geometry is the language of these forces?

Could the insertion of genes to cause cells to make certain chemicals{that kill viruses and dont harm cells} kill viruses? Also, what is it in soap that kills viruses and bacteria but doesnt harm skin cells? thanks.

Are his ideas taken seriously in the Biology field? What is the concensus in the scientific community regarding SENS?

how are viruses slowed down? i know that fevers do this to colds, but then why not do this to other viruses too? is there a way to slow down virus mutations? if so, couldnt you cure hiv? thanks.

Hi everybody i am a new member and this is my first post i am a student 2nd year faculty of medicine in Egypt i need some animations for glucose transport Thanks

I am working with murine cel lines and compare their responsivenes to alpha radiation. Will be happy to share knowledge and ideas.

Dear all, does anybody know how to convert an enzyme activity of e.g. 5.1 IU/mg to a reaction rate, let's say in micromol/s ? I read that the IU unit for biological activity and it's definition differs from substance to substance. Does anybody have a guess how I can get information of how to convert this for my specific enzyme (creatine kinase)? Thanks in advance, cheers Hans

Hi! I am currently trying to perform an LR recombination reaction between the positive control of the LR reaction kit pEntr-Gus(donor vector) and the entry vector pDest32. I am trying to perform this reaction successfully and then try to do it with the actual cDNA library, which is already put in a donor vector. For some reason I cannot make my positive control work. I believe that it is a problem with the LR reaction, and not the transformation of the reaction into DH10B cells, because my transformation of the cells with the positive control pUC 19 works perfectly. It is just that they cannot be transformed by the DNA in my LR reaction. Could it be that all the cells ar…

what are the types of microscopes used to look at things like viruses or cells? can you use an optical microscope? i read somewhere that optical microscopes magnify things up to 1500x. is that enough? or do they use electron and scan tunneling microscopes for this? how much would it be for a telescope capable of this?

First, its nice to return to these forums after a few months and still see so many familiar faces. So my friend the other day told me his girl friend told him she "loved him but was not in love with him." This got me to thinking... what are the chemical and physical properties of love? What chemical process occurs when one sees someone and instantly feels a "crush" while he can look at a super model and be like, eh? How can someone feel attached, "love you," but not be "in love?" I remember reading some time ago about a chemical called oxytocin that causes one to feel attached through experiences, but im not sure if that all that is to it.

i know that there are severall types of pathogens, but is one or more of them actually considered to be alive? if so, how? there so small, i thought that they can only be inconveniant shapes that get in the way. they have no mightocondria, and surely there too small to have any thought process that we know about{let alone any organs, or blood for that matter i beleive} so how could they be considered alive?

Hi, I'd like to know how DNA polymerase moves along a strand of DNA? To be more precise, what mechanism physically pulls it along, and how it keeps it's speed in sync with the process of adding nucleotides?

Hi everyone I am a biochemistry student in USA. I need to sequence the newly synthesized 47nt RNA by using the enzymatic digestion method. Does anybody know where i can find the enzymatic RNA sequencing kit? Thank you very much

what will be the role of wd40repeats or leucine rich repeats in dengue diseased person as proteins containing wd40repeats are up regulated in the dengue and Parkinson diseased persons ... .. how can we co relate working of these domains with the diseased condition. intense conditions of these disease have to deal with environmental condition and hope fully these will be deciphered .. any ideas about this please reply

While on another site reading an article on photographic memory. A simple question came to me. It was "can one improve one's memory without using some sort of trick to it?". For example, without using some sort of mnemonic. So, if one were to constantly memorise things...let's say definitions from a dictionary, will one's ability to remember in that specific way improve as a result of repetition? Or is our ability to memorise things based purely on genetics? Or is it a bit of both? BTW, I have heard that many people do not believe in the so-called photographic memory. Any theories, or ideas behind it? Soz, if the first question seems naive, anyway I'd love to hea…

Hello, I ran a electrophoresis for a intro bio university class. Each group was given two ladder, and two wells, one to use with ECOR1+DNA and one with just the DNA. One group came up with a question that stumbled me; they forgot to put their ECOR1 in their DNA (or just didnt give it enough time to do its magic), but now they have two strands with the same length. The reason I feel the need to help them is because the ladder I filled leaked into their wells, and they ended having three lines show up, two perfectly matching the ladder, and one perfectly matching the uncut. This resulted in them having a rather hard time figuring out how they could …

Hi. I have two questions about lipoprotein metabolism in rats and having trouble approaching them. Any help would be appreciated. 1. Rats are nocturnal feeders. How does this effect on the study of lipoprotein metabolism if the samples are collected during the normal feeding period of humans? 2. If the rats are given less chow to consume, how will this effect the VLDL metabolism of the experimental group (on liquid sucrose diet) and the control group (normal diet)?

I was wondering........DNA is touted as being the master substance to identify idividuals but it has a variance of only FOUR different components, C G A and T. How does this get arranged so that every individual organism has a different arrangement. Secondly the genetic code is degenerate, yes? But is there any other advantage of this besides that any effect of a mutation is ameliorated? Thanks people.

We're planning on expressing a sequence that contains an insertion of two copies of a gene in a tandem repeat. Should we be concerned with recombination or 'slippage' and loss of a copy during cloning and expression in XL2-Blue/BL21 DE3 E. coli systems? We have the option of using it from another organism, but it would be a eukaryote and we are concerned about codon optimization and poor expression.

I hope this question will not be too stupid. I am just very interesting in this subject and I am a beginner about biochemistry. Well. I found a new health supplement. It said people need to supplement extra 8 acid (Citrate, Isocitrate, Oxalosuccinate....) to make TCA cycle work perfect. What I know is TCA cycle produce these acid step by step and what will happen if add extra acid in it? I just think it may break down the TCA cycle....or I am wrong???

Is there an evolutionary purpose/advantage of having uracil in RNA rather than thymine (as seen in DNA)? Why does only thymine get replaced in RNA? -cheers

I've to give a presentation on transcription so i want to know about all the detais for the process of tanscription in both prokaryotes and eukaryotes.