shu zhou

I want to sequence a 47 nt RNA on a 40cm long gel plate. What is the percentage of the acrylamide should I use to resolve all the 47 bands on the single gel at the same time? And what is the difference between Acrylamide:bisacrylamide ratio 19:1 and 29:1? which ratio is appropriate for me? I tried 12% 19:1 Acrylamide : bisacrylamide gel. the bands between 35 to 47 were not separate. Thanks a lot

I purchased fluorescent labeled RNA. And I want to detect the RNA concentration using 260nm reading. But I need subtract the fluorophore effect at 260 nm using equation A260corre=A260-(Amax*C.F.260), where i can find the C.F.260 for different fluorophores? Especially TAMRA and ROX? Thanks a lot

Hi everyone I am a biochemistry student in USA. I need to sequence the newly synthesized 47nt RNA by using the enzymatic digestion method. Does anybody know where i can find the enzymatic RNA sequencing kit? Thank you very much