ChazR Posted March 16, 2009 Share Posted March 16, 2009 We're planning on expressing a sequence that contains an insertion of two copies of a gene in a tandem repeat. Should we be concerned with recombination or 'slippage' and loss of a copy during cloning and expression in XL2-Blue/BL21 DE3 E. coli systems? We have the option of using it from another organism, but it would be a eukaryote and we are concerned about codon optimization and poor expression. Link to comment Share on other sites More sharing options...
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