Biochemistry and Molecular Biology

Can anyone tell me, or give me a link where I can find some information about the influence of diferent types of sugar (lactose, dextrose, frutose, arabiose and sucrose) in the growth of bean cells? tks

Hello! I got a question. I can not understand what is the exact function of the alpha subunits in the proteasome. It is written that it maintains the structure of the proteasome and is regulated by "regulatory particles" what are these regulatory particles and isn;t the 19S subunit that is responsible for the recognition of the polyubiquitin and so on?Thank you.

Hello again. Well I got a problem... I am looking at the reaction which transforms succinyl CoA into succinate... catalyzed by succinate thiokinase. There is a molecule H20 that is not included in the scheme at the book. My professor said that there is a lot of water and hydrogen in the cell and that is why it is not included. If it is so, why do the cell need NAD.H as a special transporter of hydrogen. Well id u can enlighten the matter a little bit I would be greatful.

Hello, I got a problem. I don't know the difference between glycerol and glycerate, "ate" worries me. If someone can explain it i would be very grateful. Sorry bout the dumb question, i am learning the basics.

hello, PhospholipaseA2 is a enzyme that cleaves fatty acids (principally arachidonic acid) from the second position (F2) of phospholipids, and arachidonic acid is the precursor of Prostaglandins(PG) and Thromboxane (TX). We know DAG(diacylglycerol) is important in the G-protein linked intracellular signal transduction ( in the Proteinlipase C (PLC) pathway, PIP2 is breaked into DAG and IP3 with the catalyse of PLC), 1- Why it was said that DAG (diacylglycerol) is the precursor of PG ?(http://www.answers.com/diacylglycerol?gwp=12&method=2) 2- is there any relation between Proteinlipase A2 and the synthesis/dissociate of DAG. 3- What (if there is ) role…

Fill in the blanks. The first workable fraction in the enzyme purification is the __________. Each subsequent successful purification step should show an increase in ____________ and very little decrease in __________________. To keep track of all proteins during each step, we would typically run a general protein assay, such as the ________, which utilizes the dye Coomassie Blue, which binds to ___________ groups in proteins. What I have so far: 1. Crude 2. Not sure if it's specific activity or fold purification. 3. 4. Bradford assay 5. Hydrophobic

I'm studying for my biochemistry final and encountered a pH problem that I don't know how to approach. A compound is known to have a free amino group with a pKa of 8.8, and one other ionizable group with a pKa between 5 and 7. To 100 mL of a 0.2 M solution of this compound at pH 8.2 was added 40 mL of a solution of 0.2 M hydrochloric acid. The pH changed to 6.2 . Find the pKa of the second ionizable group. The only way I know how to attack this is with Henderson Hasselbach but I haven't been able to solve it yet.

Hi guys, I was wondering about the feasibility of combining two domains from different proteins together. What sort of results can i expect? How often would I end up with a fully functioning protein? I'm thinking about giving a non-specific enzyme with a domain that allows endonucleases that cut specifically

http://www.studiodaily.com/main/searchlist/6850.html click on [low] for the low resolution version, still sharp

A student wants to purchase some trypsin to use in a protein sequencing. Company A offers 200 I.U.'s of the enzyme with a specific activity of 405 I.U./mg at a price of $200. Company B offers 150 I.U.'s of the enzyme with a specific activity of 450 I.U./mg at the same price. Company C offers 405 I.U.'s of the enzyme with a specific activity of 200 I.U./mg at the same price. Which company(s) should you buy from? I get Company A and/or Company C. Is that correct?

Hello Guys, I'm in to coin collecting and i don't know which specific microscope i could use for my hobby. All suggestions and tips are most welcome. Thanks in advance.

I'm doing a project on the purification of sheep platelet cyclooxygenase and have read a paper. But there are some steps of the protocol which I don't understand. First, hydrophobic chromatography using ibuprofen-sepharose affinity column was used. Next, fractions were subjected to 2 metal-chelate chromatographies - IDA/Zn column then TED/Zn column. Cyclooxygenase binded to IDA/Zn which was eluted with imidazole. The cyclooxygenase-containing fractions were put through the TED/Zn column but cyclooxygenase did not bind and was collected in the unbound effluent. The unbound effluent was then put through a Haemin-Sepharose affinity chromatography column and purified …

Are we still debating on if GM foods are good or not? Cause the pro side is winning I think. All the cons ideas are mostly theories like questions with IF with them.

I've been wondering something: How does limiting the amount of carbohydrates in the human body change the metabolism, chemistry, and other physical processes of the human body? For what I understand, the body breaks down sugars and eventually turns them into ATP. However, I've been wondering, if the body doesn't have as much sugar, how does it create ATP? If it doesn't use sugar to create ATP, doesn't it use fat or protein to create ATP? Are other biochemical structures activated in order to render fat or protein to be transformed into ATP? I'm trying to understand why the Atkin's nutritional approach, or "Atkin's Diet," works for some people. Why does it work? Wh…

Hey everyone! Just a question out of curiosity. Humans (other animals too) appear to be very sensitive to the smell of any thiol compound (some can be detected in parts per billion). I was wondering if there is a biological reason why this is so? Is it intended for something such as a defence mechanism or is it a by product of being able to smell other aromatic compounds and such? Thanks for the information. -- Ryan Jones

Hello again, does anybody have (or knows where i can find it) the whole sequence of the pJIT 60 plasmid? Thanks

Hi everybody! Has anyone ever tried to clone the sequence SEKDEL (for ER directioning) by PCR? I was wondering if I could get some help with the primer design and some other technical issues (a link or a paper where i can find some decent description of the procedure would also be very very helpful). Thanks a lot

I've been trying hard to remember a lecture I had about 1.5 years ago. My lecturer displayed a page on NCBI about pan troglodyte chromosomes and a certain chromosome which possessed almost complete identity to the homosapien kind. The page specifically compared both chromosomes but I can't find it for the life of me. Can anyone help ? http://www.ncbi.nlm.nih.gov/

I happened along this article, and I don't know whether to be shocked or amazed: http://www.avionnewspaper.com/media/paper798/news/2005/11/15/ScienceTech/BioPaper.Breakthrough.Enables.Printable.Organs-1058600.shtml?norewrite&sourcedomain=www.avionnewspaper.com This seems to be quite an achievement for both biology and engineering, but will it work? Can organs be structured using cellular/bio-paper scaffolding? Would the "paper" provide sufficient support for the organ? And what about the various cells within an organ, how will the protocol know where to place each cell? I believe that simple organs, such as skin, can be manufactured. Skin is complex,…

I tried to find out on line a succinct explanation what mediates foetal stem cell ( presumably in a blastocyst ) differentiation into specific organs ( presumably following gastrulation ) during development but just got totally confused . Can some kind person tell me ?

This is not at all what I have understood yet it appears to be an authoritative source ----http://americanthinker.com/articles.php?article_id=5977

What happens in the stomach to trigger the nausea sensations we feel when we consume things that we shouldn't be consuming. There must be a layer of something coating the stomach walls, something that protects it from stomach acid, and when we consume things like alcohol or any kind of foriegn substance we're not used to, it somehow eats away at this layer, and once the actual stomach walls are exposed, the stomac acid starts eating away at this layer, triggering the nausea reaction. Am I right... or way off?

glucose - 1 phosphate to udp glucose. is the enzyme udp glucose phosphorylase or is it udp glucose pyrophosphorylase? Discrepancy between lecture and and online sources. Wikipedia says its the latter, my notes say it is the former.

I am trying to start up an ELISA to detect malariaparasites in the malaria mosquito (Protocol by Wirtz et al. 1987). I am trying to use the 0,5% boiled casein blocking buffer (so without using BSA). In the protocol that I have it simply states: suspend 2,5 g casein in 50 ml 0,1 M (0,1 N) NaOH, and bring to a boil. After casein has dissolved, slowly add PBS and allow to cool. Problem is, the casein wont dissolve, I tried boiling it for over 2 hours, but still without succes. Has anyone done this ELISA? I would appreciate it if someone can help me solve this problem. ELISA Sporozoite Detection.doc ELISA sporozoite Solutions.doc

typically , prokaryotes do not undergo mitosis !!!! is it true ???? and how are them goin to reproduce ????