Biochemistry and Molecular Biology

I had carried out a lab session and I am facing a big problems which I cant get through, I hope somebody here can help me. While I was getting UV absorbance at 510 nm for glucose standard curve, I get a negative reading for one of the tube. Is this possible? Why? somehow, I get negative reading for [ethanol] produced and [glucose] catabolised. Is that means no ethanol being produced nor glucose being catabolised? I had attached my lab report with this thread, hope someone can answer my problems. Thanks a lot!!! Experiment 2.doc

Hello, I was wondering if anyone could help advise me. I am trying to do enzyme inhibition kinetics but according to protocols I need to do it using the substrate to zero the spec with first and then look for a decrease at a certain wavelength. When this happens there is a decrease but the absorbance goes negative, consequently lineweaver burk plots face downwards. I do not know enough about this as this is the first time I have ever tried an enzyme assay, Can anyone suggest how I could change things? Many thanks to anyone who replies

hello. i have been working on developing a new leptin and leptin receptor ELISA as part of my honours project, and after 8 months of development i have finally gotten them to work. i have been having a lot of trouble finding the exact amino acid sequence of the peptides i have been using. can anyone direct me to a good online database i can use? thanks Erynna

I was looking at various images of structure in regards to seeds such as cross section views and what not. I was thinking about the reason for the various arrangements seeds take in that regard and how much of it has to do with aspects such as molecular structure and physical chemistry in application of larger biological structures. I would like to find if the continuity of equilibrium regarding large scale systems comes to apply here. As easy example to look at of course I think is DNA, it has a particular physical structure which relates to its function and or interaction with its surrounding environment. I was wondering about how large a scale this comes to apply,…

Human Papillomavirus, more commonly known as HPV, is a viral infection spread through skin to skin sexual contact. HPV is a group of over 100 different viruses, with at least 30 strains known to cause different types of cancer. There is currently no cure for HPV. How Can You Get HPV HPV is transmitted by skin to skin contact through vaginal, anal and oral sex with a partner who already has HPV. If infected, signs and symptoms may take weeks, months and even years to appear. Symptoms may never appear. Source:http://healthnewsnetwork.blogspot.com/2007/05/human-papillomavirus-hpv.html

I am a chemistry lover, recently found this forum, but when I tell people that I want to go and study biochemistry next year they look at me like I'm some kinda loser.They think that everyone who goes there automatically becomes a teacher.I live in Romania so maybe it's true that many would become teachers. Do I have to have connection in order to get at a research job or is this just a scare devised so I wind up in front of a PC all day, writing C++ programs?(I study at an informatics high-school) They must really hate chemistry

hi i am here looking for role of singlet oxygen in plant signalingand how DCMU effect the photosystem II in plants ,if anybody can help me out with good material for this, i will be very thankful . thanks

Hi, I am planning to do bleaching of wood using Hydrogen peroxide. I just want to know the basics. What is the color that gives brown for plant parts & also which part of the plant is removed while bleaching. Can any one help me in this issue. Reagrds, Karthikeyan

Okay, lets say you isolated a bacterial mRNA through the binding of another RNA of much smaller size (say 70bp) how would you go about cloning and sequencing this bacterial mRNA you isolated? Rules: 1) Bacterial RNA does not have a poly A tail. 2) You're completely clueless about the sequence of this mRNA you've isolated. 3) Your sample is contaminated by this small RNA by thousands if not millions of times the copy number of the RNA you're trying to isolate. 4) There are thousands of possible binding partners for this small RNA so you can't just search for the anti-sense. So you basically have to selectively clone this mRNA and sequence it. I've thought of a…

Is these ideas going to work. As we all know that the life span of chromosomes depend on their telomere length. With the help of RNAinterference can we switch on the gene in humans which synthesises the enzyme telomerase and live forever. Or else You can overexpress the Klotho gene to produce more klotho protein to increase your life span.

It's a pretty amazing discovery. http://www.nature.com/nature/journal/v447/n7145/full/447618a.html I think they even took a bunch of mouse skin cells, reprogramed them into an ebryonic state placed them together impregnated a mouse with them and a chimeric mouse was born from the cells!! Pretty amazing breakthrough...

Does anyone have any suggestions for suspending and storing elastase in aliquots and the how long it can be stored like this for? Many thanks

I'm trying to get my head around lipoproteins and have hit a stumbling block. From what I am reading 'The exclusive apolipoprotein of LDLs is apo-B-100' So I take this to mean that they lack apo-E, which would therefore mean that the LDL receptors in the liver cells (and others?) wouldn't recognise the LDL. I feel like a dog chasing its tail. I'm also wondering what the mechanism is that determines if IDLs are taken up by the liver or continue to loose triacylglycerols (and apo-E to HDLs???) to become LDLs. Any help would be appreciated Thanks, Al.

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my supervisor said that when sequencing EST, we juz sequence it 1 time and juz 1 strand...if i'm not mistaken from 5'-end... can anyone tell me why???? i'm juz curious.... :confused:

have anybody here know about 454 pyrosequencing??? is it much better than Sanger method???? :confused:

I'm curious as to what ya'll think is the most effective way of taking genomic DNA and chopping it into random fragments of a defined size. Say around 500bp. I'd think ultrasonification but 500 bp is probably to small for that. So maybe a time calibrated Dnase treatment or even a restriction digest. There's also pcr with random primers. But which of these methods would be the best to represent the whole genome in Random 500bp fragments?

I have been fascinated by a theory of mine. Is there a way to split the human genetic structure, then split and fuse another genetic strand to it in order to create an entirely new genetic sequence? I have been theorizing that if it can be done with, say, animal D.N.A, then one could in theory create a mutant genetic structure for the purpose of solving genetic defects without a human donor.This way there would be a virtually unlimited supply that could help thousand, if not more, who suffer from such defects.

i juz finished extract my RNA ... but the problem is when im check the quality of my RNA, it shows that there are lot contamination of polysaccharides(A260/230 = below 1.0).. the A260/280 is ok, i think...around 1.7-1.9... actually i want to construct cDNA library.. so, how can i get a good quality RNA to synthesize cDNA??? my fren juz tell me that i can purify my RNA before synthesize cDNA.. is it right????? and 1 more thing, if my RNA not so good , what happen if i continue to synthesize cDNA????

Hello!! I got a problem and the whole thing is a mess. I want to induce UV mutations in a gene and see how this protein is gonna work. How is this thing done?? How should I effect only the gene I am interested in??? Thanks !!!

Some say that only God can create life from scratch, but it looks as if man is about to do the same -- maybe as soon as within the next few weeks. This article says that microscopic life is about to be created by humans. If this actually happens, are we playing God?

Hey all, I have been thinking about the evolutionary accumulation of introns. Firstly, they used to be referred to as "junk-DNA" but now we know that this is not exactly true. For example, intron splicing is responsible for the K+ channels in our ears that enable us to hear different frequencies. However, could they still have accumulated as junk and only later been shaped by natural selection for specific purporses? If they were once just junk, why are there no introns in prokaryotes? They seem as good DNA carries as eukaryotes. It seems logical to suppose that the advance transcription machinery in eukaryotes requires introns, while the prokaryotes do not. This once…

I am applying for a graduate school, and I am interested in microbe-engineering. Who can do me a favor, just tell me, which university has a microbe-engineering department? Thanks a lot:-)

Hi, I am doing an electrophoretic mobility shift analysis with the outer membrane protein of a bacteria and DNA molecules that potentially bind it. These DNA molecules are biotinylated and are detected by chemiluminiscence. The strange thing is I am visualizing a reduction in intensity of the band but do not really see a shift. If I increase the protein concentration, I do get a corresponding decrease in intensity, but have never been able to see a discernible shift. If I compete it with a non biotinylated DNA, the intensity remains the same as that similar to the unreacted DNA control. However I really would like to see a band 'shifted up". I have the following questio…

im working on bacterial proteins and one of the protein contain two ligands in its structure and i dont know what the process to run the two ligands programe. im using autodock as a docking progame and for modelling modeller dynamics gromacs