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Posts posted by Bluenoise

  1. Well if the RNA is getting degraded I'd automatically think of RNase. The only explaination that I can offer for why your very young leaf is working is because it likely has a higher level of RNA in it. Faster growing cells need more RNA than stationary cells.

    So are you sure that you're redisolving in DEPC treated water, and everything is very RNase free?

    Another possibility is that the RNA is getting degraded before the Trisure is able to deactivate any RNase present. I'd recomend pulverising your leaf into a powder with liquid nitrogen prior to the addition of the trisure.

    However you may already be doing this. If so I don't really have an idea.

  2. My guess is that as the bag is heated it become more soft/flexible and collapses from both it's own weight and the weight of the condensation on the inside of it. Also condensation inside it would probably make it stick to the sausage better. Like a surface tension effect.

    You ever try to stick a wet hand into a latex glove? It's impossible. The same thing happens when you wear to big gloves and your hands get sweaty, they stick like the air has been sucked out.


    But your definition of shrink is confusing. Could you not take before and after pictures? That would help alot.

  3. I'm not sure what it is, but I don't think it is finely powdered Al or Mg. I have some of those powders and for sure, they usually don't ignite by simply blowing them in the air.


    I suspect that the persons, who did this trick still had some source of ignition in their hand. I know this kind of tricks from magicians shows and they use some flash-like powder, and they have a tiny sparking device in their hand.


    I agree if it's so volatile that simply blowing on it or some sunlight hitting it is going to set it off you probably aren't going to see anyone holding it in their hand.

  4. when you have a folder full of songs or videos microsoft has taken it upon itself to change your sorting options to those it has deemed relevent to music and videos (year, artist, title, etc.)


    i absolutely hate that because i want my list sorted by when it was modified. i cant for the life of me find a way to get the old sorting options to come back. ive tried changing the customization properties to a document folder.


    Okay when in details view when it sorts it my "artist, year, album, etc..."


    You rightclick on the tab (where it says artist, year etc...) and a menu will pop up allowing you to select which options to include in sorting. There is quite a large selection of them, with two of them being date modified, and date created.

    It seemed like a pretty obvious way to set it up for me...

  5. You'll need more information than that to answer this question.

    Like the starting pH of what your disolving your salt into. Plus there are many molecules that can donate or accept more than one proton/ (electrons)


    But assuming you're disolving in a non-buffered solution of pH 7. And the molecule only accepts one proton (because it's obviously a base).

    Then what you suggest would work for an acid.. But since the pH has raised you will need to find the concentration of the base.

  6. When talking about house plants sometimes trace elements such as iron can be usefull as well.

    But yeah nitrogen, phosphorous, and potassium mainly, esspecially for agriculture as state above.

  7. what food could i use to represent the smooth endoplasmic reticulum and it's functions


    You mean what food would work as a metaphor for the packaging and modification of proteins and lipids?


    I got to tell ya that's a strange question


    SPAM of course. Ham that's modified and packaged in a can.



    Or do you mean in physical appearance? It kinda looks like a stack of pancakes.

  8. According to this abstract


    "In length heterogeneity PCR (LH-PCR) a fluorescently labeled primer is used to determine the relative amounts of amplified sequences originating from different microorganisms. Labeled fragments are separated by gel electrophoresis and detected by laser-induced fluorescence with an automated gene sequencer. We used LH-PCR to evaluate the composition of the soil microbial community. Four soils, which differed in terms of soil type and/or crop management practice, were studied. Previous data for microbial biomass, nitrogen and carbon contents, and nitrogen mineralization rates suggested that the microbial characteristics of these soils were different. One site received two different treatments: no-till and conventional till perennial ryegrass. The other sites were no-till continuous grass plots at separate locations with different soil types. Community composition was characterized by assessing the natural length heterogeneity in eubacterial sequences amplified from the 5' domain of the 16S rRNA gene and by determining fatty acid methyl ester (FAME) profiles. We found that LH-PCR results were reproducible. Both methods distinguished the three sites. The most abundant bacterial community members, based on cloned LH-PCR products, were members of the beta subclass of the class Proteobacteria, the Cytophaga-Flexibacter-Bacteriodes group, and the high-G+C-content gram-positive bacterial group. Strong correlations were found between LH-PCR results and FAME results. We found that the LH-PCR method is an efficient, reliable, and highly reproducible method that should be a useful tool in future assessments of microbial community composition."


    Ritchie NJ, Schutter ME, Dick RP, Myrold DD, Use of length heterogeneity PCR and fatty acid methyl ester profiles to characterize microbial communities in soil. Appl Environ Microbiol. 2000 Apr;66(4):1668-75.



    The lengths varry between organism. So I see a few possibilites.


    1. You're using the same organism for all amplifications (ie one organism is over represented in your sample)

    2. The organism are very closely related

    3. The conditions of your pcr aren't right and your amplifying something else instead.


    Why don't you use the primers and compare it to sequence data (if available) from the organism your checking to determine the expected sizes.


    If you tell me the size of the band your getting I maybe able to help...

  9. Im doing a coursework, im at GCSE level. This coursework is late:-( and this doesnt really need to be included in it, but I was thinking about it. The investigation is simple: How does light intensity affect the rate of photosynthesis? We used the classic method, of pondweed in a beaker of water, with a lamp shining at it, from varied distances for a minute at each. To make sure no other factors affected the rate, we took soem measures. One was to add a spatula full of sodium hydrogen carbonate, to prevent carbon dioxide being a limiting factor to the rate of photosynthesis. what i wanted to include in my coursework was exactly how and what reacts, releasing the carbon dioxide. Sorry if this is a very low level question, its just that, I havent even finished secondary school yet, almost at college, jus need to sort these GCSE's. Id really appreciate some help. Thanks.


    This is definatley not a low level question. Much higher level than most of what's asked around here I'd say.


    Carbon dioxide actually has very low solubility in Water. However it can react with water to produce carbonic acid. Most of it is in an equalibrium between the two.


    Now if you replace one of the hydrogen atoms in carbonic acid with sodium you get Sodium hydrogen carbonate aka baking soda.

    This is why you have to store sodium hydroxide in an air tight container, CO2 will disolve in it convert to carbonic acid and react with the sodium hydroxide producing baking soda.


    Now to get the reverse to happen all you need to do is supply more hydrogen, by lets say an acid. So any acid in the solution will react with the baking soda, which gives carbonic acid. Now as carbonic acid goes up it will equilibrate with Carbon dioxide raising the CO2 concentration in the liquid. Which saturates the liquid most often as CO2 has very low solubility.


    Now the acid comes from metabolism of the plant (I believe).


    So acid from plant + baking soda = more CO2


    IE HNaCO3 + acid = CO2 H2O + salt.

  10. Well in all fairness there are some very general proteases. Protease K for example will digest just about any protein, and can hydrolyze after a few different residues. And not to mention proteosomes which will destroy pretty much any protein targeted to it.

    Most proteases are more specific though often only cleaving a specific peptide in a specific protein.


    However lipases tend to be far more specific hydrolyzing only specific residues from from fatty acid esters. there are likely some general ones too though.

  11. I can't really see any child abuse from that clip. She was yelling and very upset, but it wasn't directed at her children. Like her children were upset by it, but that doesn't make it child abuse.

    Like maybe forcing them to pray? Well how's that any different from all the other activities parents force children todo.


    It's definatley bad parenting. Maybe you could argue that she's neglecting her childrens emotional well being, and that neglect counts as abuse. But then I think you'd accuse all parents of abusing their children at some point by that arguement.


    I think your really stretching it to call it child abuse.


    Maybe I missed something I'm pretty hungover.

  12. I've certainly had (unwanted) fungus grow on LB agar before.


    It's a bit odd but the only time I had that happen to me was when I was using 3 different antibiotics in the media, and practicing extreem sterility. And I rarley work under very steril conditions.


    Ampicillin, Kanamycin and chloramphenicol.

    The fungus impressed me quite a bit so I let it grow for a long time before throwing it away. (Though I'm aware that most if not all of these antibiotics don't work against most fungi)

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