Bluenoise

Hi

 

I have a query about ordering DNA substrates for NMR...According to literature for a 1:1 ratio DNA:protein I need to titrate 10mM DNA substrate per NMR reaction. Most of the facilities that offer synthesis of oligos produce uM concentrations. Where can I order these quantites of DNA (in the UK), for NMR (I am also assuming that they need to be HPLC purified?)?

 

Any help would be muchly appreciated

I'm confused. First of all what matters is the molar ratio of DNA to protein... So just use an equal molar concentration of DNA that you're using for your protein.

Also usually when you order oligos they come as a lyophilized powder so you can disolve them yourself and select your concentration.

The type of purification you get usually depends on the length of your oligo. As there is a small error associated with every step in the synthesis these accumulate thus longer oligos will have more erros. I don't do alot of nmr so I'm usure what the tolerance of the procedure is.

However if you don't mind paying the extra bucks I say pay for PAGE purification. Or you could just do it yourself, that's what i do.

(Also the girls next door who do x-ray cystollography do PAGE purification of DNA so it should be good for your purposes.)

Typically if I start out with with a 50 - 100nmoles order (ie enough substrate to synthesis 100nm of oligios). I get ~50nmoles back after purification, redisolve that in 100uL and get a final concentration of 5mM. So you can see how getting 10mM isn't really a challange.

This is still typically far to concentrated for my purposes and has to be diluted far more.

Well not for me.

I get a "this video you have requested is not available... blah blah blah" message.

Link doesn't work.

Anyways, now that I got that out of my system… Sorry about that maybe was a tad out of line...

I'd say that we are all born with a varying degree of "predisposition for intelligence". (Sorry for lack of a better term); Also that our experiences/actions as we live develop this intelligence.

I think most people would agree with this statement.

However I think where the disagreement comes in is to the degree that genes and our upbringing affect this intelligence. Also whether there are different forms of genetic potential for intelligence. Say one person being musically brilliant with little effort but extremely inept at Math with massive effort.

Personally I think that genetically the vast majority of people are on a fairly equal playing field. Some within that majority having a slightly easier time at excelling at certain things than others, but I’d say that being profoundly innately better at something than someone else is relatively rare.

I think effort, frame of mind, and background knowledge will determine how well you build your intelligence in an area far more than genetic predisposition. Though I think that this predisposition definitely exists…

I guess what I mean to say is the “nurture forces” that determine or intelligence in certain areas tend to vary to a far greater degree than any predisposition for intelligence that we get from our genes. Thus the nurture seems to be the limiting factor far more often than nature. Though I can imagine instances where this would reverse; say when the top people in a field are compared. (As both have likely worked their asses off to get there, and likely maxed out their potential)

I would like to have a discussion about whether or not intelligence is significantly influenced by genes. This is something I'm doing for a school project and I haven't really done any research on it yet.

Well then of course you not doing any research and not knowing anything about the topic would make anyone who disagrees with you "dumb". Like anyone not "dumb" could figure that out.

Some people have told me that intelligence is in the genes. However, I think it's quite a silly notion. I mean, both of my parents are pretty dumb--my dad can hardly figure out how to turn his computer on!

Oh yet another brilliant post. Everyone know that not having computer skills automatically qualifies someone as "dumb".

And yet, I'm very intelligent. Children get their genes from their parents. So if intelligence is in the genes like my dumb friend says, why/how could I be so much smarter than my parents?

 

I told her to answer that for me and she paused and got visibly upset. Haha... got her pretty good. What a dumb girl.

Oh definatley! Alienating your friends and family is an absolutley brilliant way to live through life. Ha those "dumb" suckers!!

Geeze why hadn't I thought of that. Guess I must be "dumb"

faster?? It's pretty damn fast enough.

Umm well you can make sure the flower is fresh, the cut on the bottom is fresh diagonal cut. I think you can add certain plant hormones to keep them fresh longer. Anti wilting ones.

Alternatively you can alter genetic pathways in antrocyanin production and make them naturally that colour.

Paul http://uk.geocities.com/paulbryhanson

Oooooooo lightning bolts. Must resist urg to buy boook. arrrrghhhhhhh

I love how the first link on the page is the "buy now" link lol

and.. no one has really told me how to hide a body.. is burning the body a good solution?

Oh oh... Looks like it's no long a body. But has become the body now...

I think bluenoise was right.

I sure hope not:-(

I'm just guessing but likely there already have a hole there from previous piercings that is already healed.

Think about it. If these people have already peirced themselves to such a degree that the pain no long bothers them they're probably so full of holes that they don't even need to open up new wounds.

I'm not sure this would apply to surface piercings but it would to cheeks and tonges etc...

Also if the hole is small enough it wont bleed much in the first place.

Personally I don't bleed much from anything. It's a little wierd actually...

Hmmmm no response or any comments from anyone else??

Since Grignard reagents are metal organics, I figured this would be appropriate place to ask: Will Grignard reagents react with ethers? I know that they do with epoxides, but that reaction is favorable because of ring strain, but just your typical R-O-R' ethers.

 

Thanks!

Well no they don't.

Remember ethers are the common solvent used in a grignards reaction.

No I understand exactley what you mean. But isn't it usually the other way around? That zero's infront of a number are meaningless not the other way around.... Like 0001000 is really 1000 NOT 0001.

like i said above binary is 8, however if you take out the 0's after the last 1 in the sequence will it really matter. It ios just a shorter way, though i didnt do it with all of them

Do you mean remove the 0's infront of the first 1?

does anyone know if a hermaphrodite can reproduce with themself...and if they can, would the child be an exact clone of the parent?

I think he's talking about people. And the answer is no. (as far as I'm aware of)

Plus in response to the hydra. Alhough it can reproduce asexualy producing close of itself, as it has both sexual organs I think it can also produce sexually with itself.

And no they wouldn't be an exact clone. Although the offspring would obviously only contain genes only from parent, due to genetic recombination the offspring would contain less genetic diversity than the parent.

so its forensic evidence that would catch this hypotheical person i have described (whom is not in association with me at all). because im talking about australia... an we dont have cameras in many places... an im more talking about a situation with no witness'... because numerous times i have been in 'town' clubbing and been in a situation where i could say with 95% certainty there are no persons anywhere... but yeah.. so

 

 

what is a really good way to hide/get rid of a dead body? [if this is illegal to say then im sorry no one answer it... but im just wondering ]

You're really starting to scare me.

You sure you wrote it correctly? All the terms are of 8 except the second one which is only 6 characters.

I agree with the above post on most thing.

However I'm not going to say to stop you're hight proteins diet. If it works for you, then stick with it. I just want you to know that you can still eat fruit and have a high protein diet. What you really need to cut out from such a diet are things like white bread, rice, pasta, potatoes etc... Fruit is so low in calories that it wouldn't make much of a difference to any diet. Except that it would add tonnes of excellent nutrition.

You should probably just start eating fruit again.

Many fruit is so low in calories that eventhough it's all from carbs it wont add that much to your diet.

Vitamin C might help. It gives a pretty instand effect to mood, though may not be so long lasting.

Lol Seeing as your other posts was 9 hours ago, and thinking it was in the afternoon, maybe you should lol.

I just want to point out that you're on the other side of the world from me.....

So it was only 1am here.

this comes from

 

(1 + 10 + 100 + ... + 10^m)(1 + 10 + 100 + ...+ 10^m)

 

=(1 + 10 + 100 + ...+ 10^m) + 10(1 + 10 + 100 + ...+ 100^m) + 100(1 + 10 + 100 + ...+ 10^m) + ... + 10^m(1 + 10 + 100 + ...+ 10^m)

 

111

+ 1110

+ 11100 = 12321

 

Any it gives a sumation like that above with the number of ones in the first term being equal to m and the number of terms being m.

But it's getting late so I got to goto sleep now

repost of my edit.

It's a bit of a cop-out at the end but I really should goto sleep lol

opppsss I miss placed that first bracket lol.

so it's just 1245678901234567890.....09876543210987654321

Which comes from

(1 + 10 + 100 + ... + 10^m)(1 + 10 + 100 + ...+ 10^m)

=(1 + 10 + 100 + ...+ 10^m) + 10(1 + 10 + 100 + ...+ 100^m) + 100(1 + 10 + 100 + ...+ 10^m) + ... + 10^m(1 + 10 + 100 + ...+ 10^m)

111

+ 1110

+ 11100 = 12321

Any it gives a sumation like that above with the number of ones in the first term being equal to m and the number of terms being m.

But it's getting late so I got to goto sleep now

the "grey goo" scenario is way off

Actually I take that back it's already begun!!

It's started taking the potatoes under my kitchen counter. At the rate it's doubling we'll likely loose the east coast in about a month....

On a more serious point I'm pretty sure before we can makes something like that from scratch we'll figure out that it'd have to be just about as complicated as a living cell and we'll already be manipulating those to do the same thing...

I have to disagree with you on that one, Klaynos. Electrical forces are generated by electron flow, but atoms are held together (and apart) individually by intramolecular strong and weak forces. My guess is that the correct answer is "nuclear forces"

The answer they're looking for is electric force. Or the electromagnetic repulsion between the electron shells.

Why send more troops?

Give him a gun and send Bush instead. If he's so big on this war why not let him fight it instead.

I gaurentee you this solution will probably end about 50% of american problems.

You can do a DNA extraction from some veggies. All with stuff from the local drugstore or supermarket.