Genetics

I am having a little trouble here. I am an undergrad attempting to revise and my lecture slides are awful. Can anyone take me through the process (each step) of identifying a causative gene in an autosomal dominant inherited disease. I would like to know how it was done say 15 years and how it is done now. Any help would be great. Thank you.

In a chromosome (i.e. 2 chromatids), is it correct that there are 2 separate (and identical) molecules of DNA? On Wikipedia, the definition of chromosome is stated as "a single piece of coiled DNA" (http://en.wikipedia.org/wiki/Chromosome), but I believe they mean a single chromatid is a single piece of coild DNA, correct?

Hi, I was wondering if someone could explain the genetics behind DNA genealogy tests. What are the actual genetic markers or fingerprints that allow companies like ancestry.com to define someone as Northern European, or Native American, or East Asian, etc. etc. Thank you

I made an IBS distance matrix using PLINK and would like to use this matrix to build a neighbor joining tree in 'Neighbor' of the PHYLIP package. When I read in the resulting matrix from PLINK in Neighbor and try to run then I get the following message: 'ERROR: diagonal element of row 1 of distance matrix is not zero' What goes wrong? Can I not directly use the PLINK output? How can I solve this problem? I'm not really familiar with these methods so I hope someone could help.

Hi everyone I just recently done genomw wide SNP analysis on 20 N2 animals (SJL/ C3H cross) with over 300 polymorphic SNP`s. By using an affect only mapping strategy I plotted the number of mice which are heterozygotes against the loci of the mutation and found linkage on chromosome 8. Now the problem I have is that I have to calculate the threshold at p=0.05 for the number of animals. From a similar study I got given the following paper they have based their calculation on (see following link to access paper). http://www.genetics.org/content/138/3/963.full.pdf However since I have no mathematical background I`m completely lost by reading the paper and tryin…

This is related to another topic I posted in the 'Other Sciences' category. Basically, I am seeking a sort of 'how-to' guide for bio-engineering. Specifically, my interest lies in the engineering of plants- especially medicinal plants- and how to engineer a plant to produce a predetermined chemical in its foliage, stems, roots, flowers, fruit, etc. Keep in mind that I am an amateur scientist and have almost no equipment to use for this particular venture, I just want a basis for the upcoming experiments that I am planning, all of which involve the creation of 'new' plant organisms. To clarify, by 'new' plants, I mean the genetic modification of existing plant ce…

I was just wondering if we had a powerful enough computer would it be possible to simulate the growth of a person using an individuals DNA?

Hello all, I've been thinking: Would it be possible to make a human immortal by cancelling/modifying genes? As far as I know, it's possible in theory but would be absurdly difficult in practice. Could anyone confirm this?

Can someone explain why I am getting negative d' values using DNAsp? The D, D' and R values are all negative and I have no idea why! Please can someone reply? Thank you x

geometrically is there any advantage of dna being formatted as a double helix. (other than being wrapped around itself). for eg) if it were a spiral would that confer any advantages? Any research on this?

I've recently discovered my blood type is B+, but both my mother and my father have blood type O. I double checked it so I'm sure it's B. My parents did their blood types when they got married (at least). However, it's impossible for me to have B type when my parents both have O, because I must have gotten the B from one of them. So I'm worried that my father might not be my father. I look completely like my mother and there is no obvious resemblance with my father, apart from a wider nose tip and maybe the way my feet look. I know my mother used to fool around back in the day (yes, around the time I was conceived) with her ex-husband, which DOES have the B type blood…

I am trying to develop a laboratory test for genotyping patients on a SNP site. The polymorphism does not cause any change in restriction site (using online restriction mapping tool). The sequence is very repetitive and poor in GC. Allele specific primers are being tried but, looking for alternative solutions. Any suggestion is appreciated ? Thank you ahmer11

Ok i have this idea. I remembered having this conversation with someone on the problems on genetic engineering in the usa. i think it was about how a big farming company sued a small faming company because there genetically engineered food what breed with there food ( and it was engineered to stop growth or something). and how the government is passing a law on genetic engineering. Ok it the point. cant we do the same thing with illegal drugs to make the addictive properties less addictive or have no addictive properties and stop the growth completely. Then drop the plant where people grow there drugs. E.g. drop the seeds or pod in Afghanistan where there is a group i for…

I am generating a new mouse model and want to determine which of my animals are homozygous by testcross with wild-type animals. How many offsprings do I need to be certain that the parent is homozygous?

I'm wondering if it is possible edit someone's genome (someone already living) to produce certain phenotypes, such as hair quality. Certainly, you would have to cancel out some existing genes. But I'm just wondering if its possible. Certain examples would be like manipulating hair thickness, the location of the hairline, hair color, straightness etc.

I've come up with an idea to make transgenic flowers. My inspiration came from Glo fish, my fascination in the beauty of flowers, and being a sappy romantic. My idea is to microinject the seeds of a rose with Red Fluorescent Protein (RFP) so that when under a black light, the rose glows a beautiful red. My idea comes with a question: would this work? I've read a little, but most research for RFP is focused on animals and most research for transgenic plants focus on favorable attributes of crops. I'm not sure if this would work the same way for a flower and it would be preferable that the protein was concentrated in the petals. Any advice, enlightenment, or constructive c…

Hello, I am working on a children's fiction novel and my genetics are a bit rusty, so I would like to receive help regarding inherited traits. In the narrative, I would like for two traits to produce three outcomes. Consider the traits as F or E. Then, children from an F and an E parent would have F, or E, and very rarely, FE. Taking away dominance and recession, how do I make this work? Punnett Squares arent helping but I may be doing something wrong. Get in touch!

Hello everyone, I read about this protozoan about a month ago and it's been interesting me since. In one book, I read that it has affected about 50% of the world's population and on wikipedia I read only 33%. Whatever the number it's still extremely significant, from my knowledge it has shown to affect behavior so that comes down to over 30% of the world at least under the effect of behavioral changes or any of the parasites plethora of defects. I don't know a lot about this subject, but does anyone know quite a bit about it?

If we are supposed to be the same result of the encrypted information our genes carries, how can we expect to yeild any better result through us.

Hello everyone! An idea of uncertain value... Full reproduction through seeds is random. For some species (cherry and others), individual trees must be carefully selected to obtain acceptable fruits, and then reproduction by seeds fails. For these, one good individual tree is reproduced identically, by grafting only its branches, or by cutting or layering to get a full tree http://en.wikipedia.org/wiki/Grafting http://en.wikipedia.org/wiki/Cutting_(plant) http://en.wikipedia.org/wiki/Layering though, the last two are impossible for some species, and grafting introduces weaknesses in the composed tree. In some cases - if any possible, which I ignore - cloning the g…

When DNA replicates the 5' end of the daughter strand is shorter than the parent strand as DNA polymerase can only work in the 5'-3' direction and RNA primers are eventually removed. 5'__________________________3' 3'_______________________5' If this divides, we SHOULD end up with: 5'__________________________3' 3'_______________________5' and 5'_______________________3' 5'____________________3' So we still have ONE cell with the full strand of DNA and one cell with missing telomeres. so far is this correct (meaning: is it correct to say that the telomeres are NOT removed from the parent strand, so in each generation of divisions, there will al…

Hello there, its hard for me to grasp what is the difference between genetic interaction networks and gene regulatory networks? I mean after all the interaction happens about the interaction via proteins? Another question Im having is the inference of genetic interaction networks. What are the statistical tools for it and more importantly what data is necessary for it? I mean genetical genomics is a prospering field, but is the inference possible with expression data alone? Thanks R

If athletes who compete at the highest level in their respective sports have children. Do these children have the greatest potential for reaching the same level. How guaranteed is it, I would assume its high but I am not aware of many cases of it happening. Why is this?

I am a High School student, and I need a careers interview. I would like to be a medical scientist studying human genetics and the microbes and microorganisms we come in contact with. So, if you fit this description, could you please answer the following questions? 1. What do you like most about your job? 2.What do you like least about your job? 3.How did you get your start? What did you do during/after high school to get to where you are? 4.What advice would you give to a student looking to eventually enter your career? 5.What is your favorite type of cell? Thank you to those who take me seriously! I appreciate your time.

Aside from the example of sickle cell genotypes, what are 3 other examples of 3 allele polymorphisms in which at least 2 of the heterozygotes have greater viability than the homozygotes? Thanks!