Biochemistry and Molecular Biology

In the science community, often regarded as the predesesor of DNA and proteins, and often overlooked. Recent finding suggest that is more ncRNA than protein making RNA suggesting a bigger role for RNA. Has anyone got any opinions or more infomation on the subject. I myself beleive that RNA deserves more recognition and shouldn't be veiwed as a subserviant messager.

I think I just had a brilliant idea, and since I am feeling generous I am gonna share it with the world. Since ribosomes can catalyse the formation of peptides using amino acids from information encoded in strands of RNA, why shouldn't they be able to catalyse the formation of RNA strands from nucleotides using the information encoded in proteins? I think this may be on its way to a new hypothesis on abiogenesis..... Protein first hypothesis, I am sure there are some already out there, do they use a similar mechanism?

anyone with good links of Microarrays? thanks.

Anyone know where to find lab protocols involving transgenic animals? Specificially pigs designed to simulate human organs?

Hi all, For qualifying loading equal protein in a western blot... Is it absolutely necessary to also probe a 'house keeping' portein (i.e. GAPDH, actin etc) even though I spect all of my proteins?

hi, I´ve had some problems with getting my E.coli expressed proteins endotoxin-free. There are commercial Kits with polymyxin- colums around, but when I use them not only the LPS, but the protein as well, gets bound to the column and I loose all my proteins. HAs anybody done this? greets, kix ________________________ http://www.biologia.fi

hi guys! i am new in this field, so i hope that "mature" people can show me the way. thanks in advance! with fusion in frame does it mean that the nucleotide sequences of both DNA fragments i fuse with each other have to act as independent triplet unit? in this way we can maintain the polypeptide of both DNA fragments? in other words that their nucleotides don't come together to make the triplet codons from the start to the end no matter if the fusion is a C, N or randomly (please see example 1, below). for instance if the nucleotides of DNA fragment 1 is k's and it is a plasmid and nucleotides of DNA fragment 2 is x's. i insert 2 into 1. should i before the …

dear everybody! i am very confused about this area and hope that you can help me out of this. for example this protocol: 1. Add sufficient bovine gamma globulin to each of eight test tubes to give you a calibration curve. Suggested values are 0, 10, 20, 30, 60, 90, 120 and 150 µg/ul protein. 2. Prepare 1:10 and 1:100 dilutions of the crude TDH prior to assay.Place 20, 40 and 60 µL of each TDH solution in separate test tubes. 3. Add water to a total volume of 150 µL in all tubes. 4. Add 5 mL of diluted dye reagent to each tube and vortex (carefully). 5. Incubate at room temperature for 5 minutes. 6. Transfer 200 µL from each sample and calibrator to duplica…

does anyone know what role adenosine methylation plays in eukaryotes? iv looked through text books, papers, and asked my lecturers at uni, and as far as i can tell it seems that their is some evidence to suggest that adenosine plays some sort of role in dna replication and/or transcription, but no one seems to be sure would appresiate any thoughts/nuggets of wisdom thanx in advance

anyone know exactly where these things are located (like in what fruits, vegetables, etc?) and how they affect anti-oxidation?

is peptide bond formation a spontaneous process or enzyme requiring one. virtually in translation it is spontaneous one. but, is there a case where the enzymes can be employed for peptide bond formation?can anybody give the name of the enzyme?

hey im doing a lab report and i need to calculate the total enzyme activity for different marker enzymes. i need the molar extinction coefficient for the calculation of total enzyme activity for the following enzymes, - 5' nucleotidase - glucose 6 phosphatase jus wondering whether if u guys can help.. thank you

WIthin the next half hour i need to find a peer reviewed article on genetic engineering and its benefits, it doesn't matter what it's about as long as it's interesting and part of a respectable citable site. I don't care if it's about transgenic ducks or disease resistant beans, but it must be proffesional and peer reviewed, HELLLLPPPPP MEEEE!!!!

hello everybody! what does it mean when a gel you use in gel filtration has a "cut off 2.000 daltons" ? i have looked for the answer, but can't find it. hopes for replies! many thanks!

Can anyone tell me how porphyrins like chlorophyll and haeme are synthesized in nature. Is there a difference for plants and mammals since plants don't have haeme (i think).

What is the FDA guide line on protein A leaching ? What should be the minimum detection quantity of leachate ? Thanks in advance for replies.

Is it theoretically possible to deliver iRNA by a viral vector to suppress a particular gene? (iRNA, or interference RNA is an exciting development whereby short pieces of double stranded RNA are used to very specifically target and switch off gene expression.) If so, then would the virus continue to reproduce, thus maintaining gene suppression? Also, if the virus was infectious the gene suppression could feasibly spread between a number of hosts or whole communities. I'd better tell you upfront that I'm a science fiction writer brainstorming ideas for a novel. I know that viruses are used as vectors and presumably this would have to be a double stran…

I'm doing a huge project on the pro's of genetic engineering for "super immunity". One of the points we are arguing is that eventually the human race will be immune to various diseases due to natural selection/survival of the fittest, and that we are simply speeding up the process. However I've been unable to find even an estimate as to how long genetic therapy or even directly splicing the DNA and changing the components around takes before it is considered sucessful or complete. This is probably due to the lack of actual experimentation, but i would like to at least have a time range to place in my argument. Also if you happen to know anybody with experience in this fi…

Kind of a silly question, but would it ever be possible to create something that would make the the acidic concentration in all of the lysosomes, in an organism, so powerful that they would liquefy that organism from the inside out? Could you genetically engineer something that could do this? It would be a great biological weapon.

Could anyone suggest a substrate that is easily available for me to bind E. coli cells onto 96 well plates. I am using a recombinant protein from Bacteriophage Lambda to detect E. coli. Thanks alot for any help given.

this is more for the bio-chem side. I`m making my own cheeses, I have most all the equipment and a few good recipes 1`stly I`de like to know if rennet is 100% necesary? as the method I use (and I cant find anywhere on the net about it) doesn`t use it, although I DO have a 125ml bottle of it. here`s another question, the proccess of turning sugars and proteins into lactic acid shouldn`t give off any gasses, and yet stage one (leaving a big plastic bottle of unpasturised whole milk at room temp) will actualy expand the bottle to breaking point? enough so that the removing the lid will go BANG!!! and graze fingers so what is this gas? the Curds and whey sepera…

Hi everyone! I have a lab assignment due on Monday and I am lost with it completely, if you can, just tell me what it wants from me: Assignment: Write an SOP for the production of 100 ml of the following buffer: Recipe: 10mM Tris-HCL at pH 7.5 150 mM NaCl 2 mM EDTA 1% SDS What do I need to calculate? does it want equal grams of each in 100 ml? Ehm.. I'm looking into information I have, trying to figure out how to use it, but the thing is that I don't have the final molarity, and how can I get the grams of each component, if I have only Molarity in recipe and formula weights? it's gonna give me gms/L, not grams, coz I don't have the volumes. SOP is standa…

Hi, Im doing a A Level Project on the activity of these two different proteases when exposed to different temperatures, using photographic film as a detection method. I was going to do effect of pH but decided to do temp instead coz thot it wuld have more theory. Can someone please give me sum onfo about thse two enzymes (savinase and alcalase), structure of theirs etc to help me along...I'm goin to b usin a colourometer afta usin da photographic film..is der a gd method ne1 culd suggest? Seriously, any help at Allll appreciated please, dis is a reali importnt piece of c/w.

I'm experiencing quite some substantial problems with the BCA protein assay. I wish to determine the concentration of a his-tagged protein which have been eluted from a resin coloumn with TBS containing 5 mM EDTA in four fractions. When i meassure OD600 after incubation with working reagent the signal is rather high in the first fraction and absent in the latter three. However, when I run a SDS-PAGE and comassie stain it i see absolutely no protein in fraction 1, a nice band in fraction 2, and nice but weaker bands in fractions 3 & 4. Can anyone help me? Is it possible that stripping the resin with EDTA washes some compund, such as cobalt ions, which screws up the ass…

I need good brainstorming for this and I know you all out there may help me one more time: Is there possible to obtein proteins from chalcopyrite via microbes?what kind of microbes would they be? and how biotecholgy would be implied?