m/z

It seems that both, savinase and alcalase, are synonyms for subtilisin. Have a look at: http://www.brenda.uni-koeln.de/index.php4

Actually, I can not give you a clear cut answer to this problem. However, I would say that the SDS-PAGE results are much more specific to proteins than the BCA assay. But you could try to do a blind test - i.e. elute the resin column without loading any sample and perform a BCA protein assay of the fractions.

"The anti-foaming agent Simethicone is a complex mixture of high molecular weight polydimethylsiloxane (PDMS) oligomers (approximately 92%) with particulate silicon dioxide added to enhance the defoaming properties of the silicone oil. The PDMS is characterised in terms of its viscosity of about 60 000 centiStokes, corresponding to an average molecular weight of about 27 000 Daltons." from Moore, Douglas E.; Liu, Tina X.; Miao, William G.; Edwards, Alison; Elliss, Russell. A RP-LC method with evaporative light scattering detection for the assay of simethicone in pharmaceutical formulations. Journal of Pharmaceutical and Biomedical Analysis (2002), 30(2), 273-278. Regards, m/z

1/2 of the Nobel Prize in Chemistry 2003 was awarded to Peter Agre for "for the discovery of water channels". You can look up some information on the Nobel-Preis- website: http://nobelprize.org/chemistry/laureates/2003/ Regards, m/z

NCBI is nice. But I also like the website http://www.expasy.org/ Beside the Swiss-Prot and TrEMBL protein databases, you can also find a lot of links to different other interesting sites, tools and documentations. The Swiss-Prot database has, to my opinion, a very nice data format. If a protein is well characterized, you can find the complete precursor sequence and easily get the information which part of the sequence belongs to the mature protein, sequence peptides and propeptides. Known mutations and PTMs are given as well. To get more detailed information, it is always good to have a look into the online-documentation of the databases you are using. In addition, there are some nice tutorials in the web, which are free. See for instance http://www.bioinformatik.de/cgi-bin/browse/Catalog/Research_and_Education/Online_Courses_and_Tutorials/ Regards, m/z

Perhalogenated alkanes (CnX2n+2) are still classified as alkanes. Please find attached the CAS (Chemical Abstracts Service) entry for C2Cl6: Formula: C2 Cl6 CA Index Name: Ethane, hexachloro- (8CI,9CI) Other Names: 1,1,1,2,2,2-Hexachloroethane; Avlothane; Distokal; Distopan; Distopin; Egitol; Ethane hexachloride; Falkitol; Fasciolin; Fron 110; Hexachlorethane; Hexachloroethane; Hexachloroethylene; Mottenhexe; NSC 9224; Perchloroethane; Phenohep

I also get a ratio of Cl to P of 1.687, which is something like P5Cl3. As fas as I know, this is not a stable compound. However, the question was to calculate the "empirical formula", which means that it does not have to be a usefull stoichiometry for a single compound. You can argument that the product is a mixture of PCl3 and remaining unreacted phosphorus. Regards, m/z

The 9 isoforms for n > 0 are: 1) CH3-CH2Cl 2) CH3-CHCl2 3) CH2Cl-CH2Cl 4) CH3-CCl3 5) CH2Cl-CHCl2 6) CH2Cl-CCl3 7) CHCl2-CHCl2 8) CHCl2-CCl3 9) CCl3-CCl3 Regards, m/z