Biochemistry and Molecular Biology

Hey, I'm having trouble finding out the ways in which Vitamin B12 affects the production of hydrochloric acid in the parietal cells. If anyone can explain/point me in the direction of an explaination, I would be greatly appreciative. I know that B12 plays a role in the formation of Red Blood Cells and I know that HCl production requires ions from the blood stream, but is there a direct connection between b12 and HCl? thanks! update: since I wrote the above I did some more research and came across the nuerotransmitter Acetylcholine, which is required in HCl synthesis. Does this ring any bells for anyone? Am I on the right track?

can cells absorb extracellular atp?

Hi All, Does anyone know anything about changing growth temperature for yeast two hybrid analysis to ensure temperature is appropriate for protein conformation? I'm looking at interactions between full length plant flowering proteins that are orthologous to proteins shown to interact in 3 other diverse angiosperms (Monocots + Dicots) - so I would be surprised if they didn't interact in my species, but at standard yeast growth conditions of 30'C (manufacturer's instructions) I get no evidence of interaction from -HIS+3AT, Xgal assay, -URA. I've read articles where people have done yeast two hybrid tests at 25'C or 22'C to ensure appropriate temperature for protein co…

What do you think about the relationship between cell phone utilization and the possibility to develop cancer (particularly in brain...)? I have heard many different opinions, ones claiming that there is indeed a higher risk, while others stated that the radiation levels are insignificant.

We have been using SDS PAGE for quite some time for our recombinant protein Mw 20 kdA USING 15% Resolving and 4% stacking and tris glycine SDS Running buffer i.e laemelli system. is there any new methodology for SDS page that will increase resolution and help in getting clear sharp bands, any different buffer system or any such method. we do not want to use precast gels and cast our own gels. Also we use discontinuous Mode of buffer system. Any suggestions are welcome. regards

I'm just curious if anyone has worked with phage display before. It's a completely new technique to me, so I'd appreciate any insight you could provide on your own projects, or what you've heard/seen done with phage display. Specifically regarding phage types you've used, proteins you've worked with, and library construction/screening. I know it's been around for a while, and there are a variety of iterations of it, so discussion of any aspect of it is welcome. Opinions regarding the technique would also be interesting...especially your thoughts as to why it's fallen out of favour in recent years. A bit of background on my own project: - just starting work o…

respected members i am too confused these days.................cant decide which one is a better subject --microbiology or biochemistry??????according to u people which subject i shud go for in msc as this is my last year in bsc honours of microbiology................i have a great interest in biochem than microbio ...........but i heard microbio is more flourishing n demanding field so wt shall i choose

Do all cellular membranes have transport proteins? I ask this question in an attempt to identify some of the evolutionary difficulties the "first cell" would've experienced.

the magnesium ion gets into the cell via the magnesium/ATPase pathway, if we wanted to get magnesium in through another pathway, if we bound magnesium to something say an amino acid, would it be possible for the magnnesium+amino acid compound to get into the cell via the amino acid pathway?

Simple biochem question: how do you determine if a growth factor/protein is activated? Just if it is phosphorylated? If that is right, then is PCR the best way to probe for that, or are there better techniques? Thanks!

check this out

friends my knowledge is quite weak regarding the competitive exams taking place in india after bsc honours microbio to go for msc in microbio ,,,,,,,,,so kindly inform me about all the exams

What do "gp"and"120" in "gp 120 coat protein in HIV" mean?

Hi i need your help by suggesting me a topic on which i can do a research on in biochemistry area to get a bachelor' degree in Applied Chemistry (Biochemistry option) look forward more and helpful suggestion Alvin

I am trying to get kinetics of one nucleotide incorporation;I used a 32P-5end primer (29-mer), hybridized with a template as substrate; differentdNTP concentration, at fixed time and DNA polymerase concentration. The reactionwas stopped adding the same volume of the Loading buffer (90% Formamide, 50mM EDTA,0.01% Bromophenol Blue). Samples were heated at 95 degrees for 3 min and thenput them on ice. Samples were loaded to Polyacrylamide Gel (7.5%Acrylamide:Bisacrylamide (19:1), Urea 8M, 1X TBE, 0.05% APS and 0.03% TEMED),the gel was resolved at 2400 V until the Bromophenol bye reached the bottom ofthe gel (45cm). Gel was dried. To quantify the primers and the products, one…

i'm planning to do a relative quantification and from what i know, i have to perform standard curve for each genes that i used in qPCR. actually, i have about 25 genes of interest. so, do i really need to perform the standard curve for 25 genes??

Hi All, May I know what are techniques and concepts involved in locating the sulphide bonds in proteins? Regards Alfredo

what are the pros and cons of using an enzyme assay kit over immunoblot experiments?

Hi everybody, I'm new to this forum so I briefly introduce myself. I am a researcher at the Department of Biochemistry of the School of Medicine at Buenos Aires, working in the area of electrolyte transport in renal proximal tubule cells. My project deals mainly with the effect of dopamine (DA) that acts as a physiological regulator of Na,K-ATPase activity in the proximal tubule. Now, I have confirmed that DA increases the concentration of reactive oxygen species (ROS) even at minute concentrations, a result that moved me to explore both catalase (CAT) and superoxide dismutase (SOD) enzyme activities. I have measured CAT and SOD in cells exposed to DA and fo…

Hi i have an essay title which goes like this : Using named examples of pathogens and the mycoses they cause, discuss the importance of fungi as agents of human disease. And I was wondering is it talking about fungi only ? or other organisms that cause disease as well ? what type of things should I include in this if anyone has any ideas ? thanks

Just wondering has anyone induced beating in H9c2 cells? Dont know if it is possible, cant find anything about it on the web. would really appreciate some help!!!!

Hi All, Im trying to prepare a standard curve to estimate the concentration of amphotericin (much like a protein or lipid assay), does anyone know of a kit that allows this? I've been googling away without much success. Thanks

Okay so I have a school assignment on the morality of the whole genetic engineering scene, Its the good old debate, is it moral? Who out there is willing to give us some opinions, I do ask though that you post whether or not your happy to be quoted on your opinions, Best regards The Corporal

Super-Saturated solution of CO2, at high Temperature & Pressure (e.g., deep-sea volcanic vents), could "inject" CO2 into Reverse Citric-Acid Cycle, "driving" it around ?? The reverse Citric Acid Cycle incorporates CO2, from the surrounding aqueous medium. So, if that solution was super-saturated, with CO2 -- such as could have occurred, 4.5 Gya, in the earth's early oceans, pressed down by a then-still-super-dense-and-CO2-rich sky -- could CO2, "seeking out" of the super-saturated solution, "flee into" the reverse CAC, and "drive" that anabolic pathway through successive cycles ?

I am a PhD student testing the bioactive effect of various polysaccharides on colon cancer cells. I am using the wst-1 cell proliferation colorimetric assay to assess whether the pectins are killing my cells. I add the pectins in medium onto adhered cells, incubate for 48hrs, then add 10ul of wst-1. WST-1 is a tetrazolium salt that when in contact with metabolically active cells gets cleaved to formazan by mitochondrial dehydrogenases. I then mix the plate for 5-10mins and read on a plate reader. I have been taking readings every 2mins from 10mins to 1hr, then every hour after that My main question is this: How do you know when the best time is to read th…