MatFG Posted February 19, 2016 Share Posted February 19, 2016 Quick question here, does anybody know of a way to dissolve polysaccharides that does not affect DNA. It must leave DNA completely intact. Link to comment Share on other sites More sharing options...
CharonY Posted February 19, 2016 Share Posted February 19, 2016 Do you really mean dissolve? If so, water would be the most commonly used solvent. Link to comment Share on other sites More sharing options...
MatFG Posted February 22, 2016 Author Share Posted February 22, 2016 I have already tried water and the result, when the DNA was quantified, was miserable. Link to comment Share on other sites More sharing options...
John Cuthber Posted February 22, 2016 Share Posted February 22, 2016 (edited) There are lots of different polysaccharides and to dissolve some of them- like cellulose- you need to get rather aggressive. To be sure of dissolving all of them will be a problem in its own right/. I doubt it's possible to do that without destroying DNA.What are you trying to do? Edited February 22, 2016 by John Cuthber Link to comment Share on other sites More sharing options...
MatFG Posted February 22, 2016 Author Share Posted February 22, 2016 Get rid of all kinds of polysaccharides present in the aerial part of a cactus plant. They are contaminating my sample and I was looking for a way to degrade them to something soluble in Trizol, chloroform and ethanol that I use to wash my samples. Link to comment Share on other sites More sharing options...
hypervalent_iodine Posted February 23, 2016 Share Posted February 23, 2016 Google scholar has plenty of papers with methods for doing this. You might just need to move away from Trizol and use another extraction method. For example. 1 Link to comment Share on other sites More sharing options...
MatFG Posted February 23, 2016 Author Share Posted February 23, 2016 The obvious answer eluded me. Thanks man! Link to comment Share on other sites More sharing options...
John Cuthber Posted February 23, 2016 Share Posted February 23, 2016 It may be possible to remove unwanted stuff with enzymes. Link to comment Share on other sites More sharing options...
MatFG Posted February 23, 2016 Author Share Posted February 23, 2016 Yeah, I thought about that but wouldn't the enzymes also chow down on my DNA? Link to comment Share on other sites More sharing options...
John Cuthber Posted February 23, 2016 Share Posted February 23, 2016 Without doing the experiment, it's impossible to say. However a lot of enzymes are remarkably specific in their actions. Link to comment Share on other sites More sharing options...
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