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how to determine amount of proteins in horse's blood ?


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#1 Batbilegt

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Posted 5 January 2017 - 06:32 AM

Hello. What kind of method fits to work on blood


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#2 Function

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Posted 5 January 2017 - 07:56 AM

Amount of protein? Same as humans, I think ... Electrophoresis of serum proteins


Edited by Function, 5 January 2017 - 08:20 AM.

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#3 CharonY

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Posted 5 January 2017 - 09:44 AM

Amount as in different types of proteins? Electrophoresis does not have the resolving power to do that (and even 2D would be too limited). Or are you talking about total abundance? In the latter case any protein quantification method (Lowry, BCA, Coomassie etc.) would work. Most of it is going to be albumin, anyway with a decent chung also being hemoglobin, IgGs and so on. 


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#4 Batbilegt

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Posted 5 January 2017 - 11:21 AM

Im trying to prepare soil fertilizer from horse's blood. So it needs total abundance and different types of proteins. 


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#5 CharonY

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Posted 5 January 2017 - 01:09 PM

Why is the composition relevant when used as fertilizer?


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#6 John Cuthber

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Posted 5 January 2017 - 04:56 PM

Total nitrogen is probably easier and at least as relevant

https://en.wikipedia...Kjeldahl_method

or

https://en.wikipedia...ki/Dumas_method

are commercially available.


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