Hi there,

Was hoping someone could offer some assistance. I need to measure deferoxamine mesylate concentration in solution. Is there any way that this can be produced spectrometrically?

What else is present in the solution?

Is it in clean water or urine or blood serum or something?

I strongly suspect that, in the presence of excess iron, you can measure the stuff spectrophotometrically.

If memory serves it is a siderophore? In that case you could consider a simple CAS (chrome azurol S) assay. Provided as John mentioned that you do not have too much in terms of interfering substances.

Hi thanks so much for your replies. it's just in PBS.So If I added FeSO4, I'd be able to measure it fine? Does it matter if I add a large excess?

I haven't looked into any of the details, but a quick google search shows that people have done HPLC analysis on the compound. HPLC would be a fantastic and pretty reliable way to do it. Interfering proteins or not. The free form absorbs at 226 nm, so it may be a little bit of a challenge with regards to your mobile phase to make sure something wouldn't absorb there too and to get a good signal since it is a low wavelength and noise may be an issue. I have however done simple UV absorbance of carbohydrates that have poor UV absorbance and had to use 210 nm. It still worked. If you chelate it with Fe(3+), the iron bound form can absorb at 430 nm which may make it easier if you have to use an organic solvent system.

Edited December 8, 201312 yr by sialic acid