Nashyboyo

I'm writting my dissertation and i've come accross a mental block and its driving me mad. I did some work in the lab and now i'm writting it up and i've totally forgotten how I come to one conclusion I made. I decided on an appropriate ligation teperature using two simple factors 1) the optimum temperature of the ligase was 40 degrees C 2) the laws of thermodynamics I can't remember which law I thought of and its soooooo simple its driving me mad. I'm getting confused over something so trivial can anyone help me out. By decreasing the temperature of a system you lower the random motion of particles which therefore lowers the probability that two reactants come together in the correct orientation for reaction...right ? Therefore increasing the temperature of that system would be favorable for ligation between two DNA molecules right ? I have written down in my notebook "Rondom motion doesn't favour two molecules coming together" WHY DID I WRITE THAT. WAS I RIGHT ? P.S. i'm hungover

THe official meter is marked on a platinum pole and stored in Paris

I share your amazment. The retina is what interests me the most. The biochemical events that occur there are phenominal!

Yeah that's right. You have to construct a calibration curve using the ladder to estimate the Mr of the protein(s).

7. Co-dominance is all i can think of 6 letters ? i give up

The mass/charge ratio is what separates proteins of different sizes. I can't think of the formula. The bigger the protein the more SDS associates with it thus more charge. More charge gives more mobility towards the anode and away from the cathode.

An iron reducing gene? huh? What does that mean? Am I being stupid?

I know nothing about memory of events being passed on through the generations. Interesting topic though. DNA is suprising us every day. I'm willing to keep an open mind to the possibility.

Does this mean that if all the silver chloride is used up then all that remains is a solutoin of chlorine? Isn't chlorine a gas? What's left behind must be a salt of chlorine or bound to some non-metal ? If true what would that be? Cheers

haha i should have checked the date. Damn it I thought he was gonna talk on the 17th of this month. I'll have a look around for what he said about that stuff now.

http://www.newscientist.com/article.ns?id=dn6151 Has he gone over the hill? Or do you still have faith in him still?

HAHAHAHAHAHAHAHAHAHAHAHAHAHAHAHAHAHAHAHAHAHAHAHAHAHAHAHAHAHAHAHAHAHAHAHAHAHAHAHAHAHAHAHAHAHAHAHAHAHAHAHAHAHAHAHAHAHAHAHAHAHAHAHAHAHAHAHAHAHAHAHAHAHAHAHAHAHA HAHAHAHAHAHAHAHAHAHAHAHAHAHAHAHAHAHAHAHAHAHAHAHAHAHAHAHAHAHAHAHAHAHAHAHAHAHAHAHAHAHAHAHAHAHAHAHAHAHAHAHAHAHAHAHAHAHAHAHAHAHAHAHAHAHA HAHAHAHAHAHAHAHAHAHAHAHAHAHAHAHAHAHAHAHAHAHAHAHAHAHAHAHAHAHAHAHAHAHAHAHAHAHAHAHAHAHAHAHAHAHAHAHAHAHAHAHAHAHAHAHAHAHAHAHA HAHAHAHAHAHAHAHAHAHAHAHAHAHAHAHAHAHAHAHAHAHAHAHAHAHAHAHAHAHAHAHAHAHAHAHAHAHAHAHAHAHAHAHAHAHAHAHA HAHAHAHAHAHAHAHAHAHAHAHAHAHAHAHAHAHAHAHAHAHAHAHAHAHAHAHAHAHAHAHAHAHAHAHAHAHAHAHAHAHAHAHAHAHAHAHAHAHAHAHAHAHAHAHAHAHAHAHAHAHAHAHAHAHAHAHAHAHAHAHA HAHAHAHAHAHAHAHAHAHAHAHAHAHAHAHAHAHAHAHAHAHAHAHAHAHAHAHAHAHAHAHAHAHAHAHAHAHAHAHAHAHAHAHAHAHAHAHAHAHAHAHAHAHAHAHAHAHAHAHA HAHAHAHAHAHAHAHAHAHAHAHAHAHAHAHAHAHAHAHAHAHAHAHAHAHAHAHAHAHAHAHAHAHAHAHAHAHAHAHAHAHAHAHAHAHAHAHAHAHAHAHAHAHAHAHAHAHAHAHAHAHAHAHAHAHA HAHAHAHAHAHAHAHAHAHAHAHAHAHAHAHAHAHAHAHAHAHAHAHAHAHAHAHAHAHAHAHAHAHAHAHAHAHAHAHAHAHAHAHAHAHAHAHAHAHAHAHAHAHAHAHAHAHAHAHA HAHAHAHAHAHAHAHAHAHAHAHAHAHAHAHAHAHAHAHAHAHAHAHAHAHAHAHAHAHAHAHAHAHAHAHAHAHAHAHAHAHAHAHAHAHAHAHAHAHAHAHAHAHAHAHAHAHAHAHA OH...oh i can't breath

Could you expand on this please I don't understand how this is so ? If the organellar DNA is required for co-localisation then it will remain. How is this a measure of time? http://www.journals.royalsoc.ac.uk/media/pe6ptnwrwl0f73yyrqau/contributions/9/f/l/d/9fldlqblxbxg0y5u.pdf The chloroplast retains more DNA for redox controlled expression (co-locally) for the principles described in the above journal.

Increasing the partial pressure of atmospheric oxygen by approximately 1% would cause the whole atmosphere to be flammable. The world would explode.

I remember studying that in school. I now study it at Cardiff University and I can tell you that its a lot more complicated at this level. It wouldn't be practical.

Yes its true. That is the reason why water bought from the shop in plastic containers has an expiary date.

Ever watched 24? Awsome program. This page explains what a dirty bomb is. I hope i'm not fueling some sort of terrorist attack here guys ??? http://science.howstuffworks.com/dirty-bomb1.htm

In my personal oppionion I think Duesberg is full of shit. He's saying things like Why is the increasing crime rate in bangladesh correlating with the number of baked beans I spill on my shirt every meal time! I just think the guy wanted some attention. He got it!

Urine is merely a constituent of piss. The concentration of the contribution varies with each person and each piss. The volume of the S-bend will remain constant due to the design of a toilet, thus taking a piss effectively replaces a bladders worth from the s-bend with the new contribution. The concentration of each constituent of piss will thus alter constantly. The colour and the sent will change by each day due to each person probably having a different diet or maybe a disease. Correct me if i'm wrong but i'm sure 'local' bacteria (i.e. not bacteria from the salt lakes of canada or some random underwater volcano) would survive in piss, which is why it's sterile in the first place. Therefore a solution of piss in your toilet bowl can remain there but is unsightly waste and smells. This is just an educated guess.

The effect of alcohol (ligand) on the nervous system is proportional to blood alcohol concentration because the inhibitory affect on enzymes (macromolecules) involved in neural cell precursors is cumulative. Different target enzymes bind to alcohol with different binding constants, hence the cumulative effect. This paper is a bit complex so maybe just skip to the conclusion. http://www.jbc.org/cgi/reprint/250/8/3001

i think you've answered the question now guys, lets not reiterate. How about a cup of tea ?

It depends on the neuron. 1)thicknes 2)myelinated

The link Sorry I got tired and just read the title at the top of the page and assumed the text would be sufficient. Chimps/humans vs bacteria Whether there is a 40% difference or 2% difference between genomes there is still a sufficient difference to give rise to the phenotype in question. This is an attempt to answer the first post in this thread.