Biochemistry and Molecular Biology

Anybody have experience or know how to grow/ferment these bacteria? Bacillus subtilis and bacillus amyoliquifaciens. They are used to break down organic fats in sewage works. What sort of substrate is best to grow them in a vat? Thanks.

Why do some people who have heart transplants also develop personality changes?

Hi everyone! First time poster! I am an undergraduate student studying molecular biology. I am certain you have all heard NASA's recent announcement wherein they discovered lifeforms which can utilize arsenic in lieu of phosphorous as a fundamental component of their genome. I've always wondered what possible elements life systems can consist of, and when I say life systems, I don't just mean the ones that exist on earth, but POTENTIAL life systems anywhere in the cosmos. I have never felt that life is limited to the compounds of us earth-based creatures, and that somewhere out there, elsewhere in the universe, are creatures with a TOTALLY UNIQUE biochemistry…

Hi guys! I hope this is the right place to post this! I am posting because over the last three weeks I have failed to get any data from microscopy. My problem is that the BrdU incorporation/immunostaining is not working. I have been doing this protocol for the last 4 months without any problems using U2OS Cell line. Recently, every time I have been takeing the slides I made down to the microscope, the BrdU staining is all over the field of view and it looks the nucleus has "burst" and the DNA is spread all over the coverslip. I repeated it all again today using fresh reagents and this time I couldn't see anything at all. I should also note that I have failed to …

Once a fertilized egg begins dividing, at what point do the new (daughter) cells start specializing ? For example, do all nerve cells originate, from one of the 4 cells in the embryo, after 2 divisions ? By what means, do identical cells "sort out", the "who does what" ? What is the "ok, my line will produce the nervous system", "hey, ok, I'll do the digestive track", ..., etc., "sorting out" procedure, amongst developing embryo cells ?? (Thanks in advance, for any answers, to my questions.)

ca you do it if a person is dead can you give life back can you give/create a new life?

Hi everyone, Another question. In writing about ninhydrin and amino acids that form the subunits of proteins i was wondering what it is that makes proline produce a brownish colour? From what i've read i think the purple that it usually gives with primary amino groups is due to the ninhydrin reacting with the N in the amine. I'm guessing, cos proline has a ring structure that makes the anime a secondary the N isn't available for it to reaction and form the purple... Is the above correct and what makes it go brown? Thanks for any help

I had an impurity in my sample after a BglII digestion followed by CIP. To attempt to assess the problem with a quick gel, I ran in the first lane the original plasmid straight from the miniprep, a sample containing the plasmid cut by itself, and in the last lane the sample cut with BglII and CIPed, showing the impurity at around 2kb. the marker is a 1kb ladder with bands at 10, 8, 6, 5, 4, 3, 2, 1.5, and 1k and 500 bp. Here is a picture of the gel im talking about: Now I know that when you run an uncut plasmid you typically get 3 bands. Nicked/open, linear, and supercoiled. The sample however contains those 3 bands, and a 4th unknown band. Note that when I…

Hi folks, I have some diatoms in culture and I am looking for literature about lysis and subsequent separation of cell components (membrane from cytoplasmatic constituents). I was wondering if anyone here has worked on that topic, or even if you have any tips. cheers

Consider Leitz Wetzlar 10/0.25 170/- objective lens. I guess 10 is the magnification? But what are the 0.25, 170 and "/-" things? Eg., for Leitz Wetzlar 40/0.65 170/0.17 we have "170/0.17" instead of "170/-" as above. Can't find any info/FAQ via googling.

Why is the homologous recombination event rare in plants as compared to animals or bacteria?

I've recently found out that i've developed lactose intolerance during college and I have finally brought myself to buy some lactase tablets. Anyway, they work great but I was wondering if they are functional not only at 37C but at 1-3C (ave fridge temp). I know they are probably engineered to be stable at low pH and at body temp, but what about in cold temps like the fridge? I figured, I could prolly just crush and drop a lactase tablet into my milk carton and wont have to worry about lactose effects. Plus I would like to save money. Hah. Anyone ever tried to do that themselves?

Hi, I am aware of basic synthesis of lipids, I would like some one to help me with the following answer: What is the difference between prokaryotic versus eukaryotic fatty acid synthesis/ lipid synthesis? You could help me with some key differences or direct me to a good paper which compares and contrasts both. Thanks !

I was wondering whether the presence of the methyl group on thymine vs uracil has any bearing on the relative stability of DNA vs RNA. Any thoughts?

I want to prepare chemically competent cells for BL21DE3 ( PLySs) cell line, can someone please email me or upload the protocol for the same, my Id is rasing02@gmail.com, I also wanted to know the protocol for preparing competent cells varies for different cell lines of Ecoli? or remains the same, meaning will it be different for Dh5alpha versus BL21DE3 ( PLySs) , I would really appreciate a quick response.

Please define chemical hazards and microbiology and provide examples. I wanted to learn about hazard analysis and food safety but I think it requires strong knowledge on chemical hazards and microbiology. Any inputs are welcome.

Hello everyone I am going to attach an SiRNA to a functionalized biotin. Then i need a way to quantify the efficiency of the reaction and i tough that a fluorescet Streptavidin could be usefull The problem is that i don't know exactly how to run the essay I tought that i can attach the SiRNA/Biotin somehwere and then reveal it by streptavidin Any suggestion? thanks a lot Francesco

i know that cell dies due to lack of energy,when it doesn't get enough oxygen,bot exactly HOW does it gets killed?does it get temporarily shutdown,or get damaged?plz tell me

Hi, can anyone help me with this, i basically dont know how to create an array of data (rank data). Does anyone know a good website which explains this well ? thanks

I wanted to perform some experiments on enzyme rates and chose amylase as it is easy to obtain. However, I wanted it in pure form so that I can perform mass/concentration-based calculations as well. Is there any way to extract alpha-amylase from human saliva? Thank you.

I am a Research Student and Need some information on Eye. How can i find the decay of the eye lens? Which fluid is used inside the eye and the eye-lens? Which proteins are used inside the eye lens that are responsible for its decay? What's the chemical structure of those protein? Hope to hear! Thanks in advance.

I've been reading 'Mathematical Biology' by J.D. Murray (Springer, 1989, 1993). Particuarly chapter 15 'Animal Coat Patterns and Other Practical Applications of Reaction Diffusion Equations'. But my supervisor tells me that the Reaction Diffisusion model, whilst useful isn't considered to be accurate by contemporary biologists and the existence of morphogens is looking more and more doubtful. I am trying to use Reaction Diffusion Equations to look at the formation of patterns on butterfly wings, but would appreciate any knowledge on how much this model is thought to relate to the real world.

Hi there! I need to know that what is the absorption level of rhodospin in the Human eye in different age. Where can i find the Graph of absorption of light by the rhodospin in different human age? Can anyone help me? I shall be very thankful to you. Hope to hear!

in molluscs, what gives chonchiolin the ability to make aragonite crystals form?

i need point wise difference Between these two except their definitions . 3 to 4 points will do it for me. Thanks for reading.