Biochemistry and Molecular Biology

If a -RNA virus were to infect eukaryotic cells, that virus would need to carry its own polymerase to first become a +RNA virus and then use the host cells machinery to undergo translation, correct? Under what circumstances would that virus choose to use reverse transcriptase and become DNA and what would the benefit of that be to the virus? Is it to replicate its own DNA by going Lysogenic and then excising itself?

.

Hello everyone, I just started as a research intern at a lab (at the undergraduate level), and I am reviewing a protocol I will be implementing, and I was just wondering what the term "batch bind" means. Does it have to do with allowing one substance to bind to another in a "batch" at a specified temperature? Thanks for your help, Robert

Hey everybody, can anybody recommend a good introduction (for a political scientist) into the biochemistry of emotions? I'm currently studying conflict situation in the Middle East and I would like to learn more about the "hard sciences" behind emotions such as hatred, fear, etc. e.g. What happens in the human brain when it experiences fear? How are those emotions triggered? (Sorry in advance for asking in such an ignorand way, but one has to start somewhere...I used the usual search-engines to find introductions, but the articles I found where all rather disappointing.) Thanks a lot in advance.

hello everybody! what does it mean when a gel you use in gel filtration has a "cut off 2.000 daltons" ? i have looked for the answer, but can't find it. hopes for replies! many thanks!

Arsenic is structurally similar to a phosphate group therefore many enzymes that require phosphate will also utilize arsenate. Predict the net reaction catalyzed by PGAL dehydrogenase if phosphate is replaced by arsenate. I dont know what i should write for this question however i did try: Arsenate structure is similar to the structure of a phosphate group therefore in Glycolysis, Arsenate can easily replace the inorganic phosphate that produces 1, 3 bisphosphoglycerate (BPG) therefore yielding 1-arseno-3-phosphoglycerate instead. The BPG molecule is expected to produce an ATP molecule by phosphorylating ADP to ATP (giving away phosphate to ADP) but because BPG i…

For some reason this seems like a lil ridiculous since I thought about it while I was driving but here goes... So the AIDS virus preferentially attacks T-cells and being a retrovirus has RNA that is translated to DNA via reverse transcriptase which in turn is incorporated into the host cell's genome which codes for the viral structure proteins. The fact that it's preferential for the T-cells means that it preferentially binds with a receptor?/glycoprotein?/sugar? that specifically on the outside of the T-cell. And now the question... Can you design a liposome with the receptors the virus recognizes incorporated into the membrane and thus will attack instead…

I thought the best place to ask my question is a Biochemistry forum :). So I have been forced to take Aripiprazole. A low dose of 5mg a day. I read that vitamin D3 is an inducer of the enzymes CYP3A4 and CYP2D6 both of which are the prime enzymes that breakdown Aripiprazole into Dehydro-Aripiprazole (an active metabolite) . Now what happens to the Dehydro-Aripiprazole? Is that broken down even more? Or is it turned into a form that can then be excreted from the body? I quote this : " Only after they are metabolized into more hydrophilic molecules, can they be excreted through the kidneys into the urine." My other question to all you biologists regards th…

Hypothesis #42 This hypothesis is designed to suggest how scanning tunneling microscope technology combined with the knowledge of the atomic structure of DNA could be used to create life from atoms in there free form. Note that this hypothesis is designed to suggest the possibility of such an experiment, not to explain the detailed procedures that would be involved. There are also many assumptions being made about the current limits and abilities of the technologies available to today’s scientists. In recent years the technology of scanning tunneling microscopes has been used to arrange various atoms into rings, characters, and even three dimensional structures. …

Hi all, I got stucked in my report of protein purification. My sample protein mixture (catalase + lysozyme in 5mM phosphate buffer, pH 7.5) is separated by DEAE-Sepharose chromatography. after separation, my fraction has enzyme activity was 217iu whereas crude sample is 135iu.That means %yield of enzyme > 100%. It doesn't make any sense 'cause %yield is supposed to be less than or equal to 100%, right? I'm quite sure that the whole experimental process there is no error or mistake at all. Does any one can suggest any solution for me in this case? Thank you so much!

I will be amplifying the variable regions for 16S rDNA and looking for Universal primers for bacteria only can some one help me with this please!!!!!!!!!!!!\ I looked up some articles but i got confused wether those primers are used for conserved regions that are the same among all bacteria or not. thanks

If the melting points of these 18-carbon fatty acids are like this, stearic acid, 69.6 °C, oleic acid, 13.4 °C, linoleic acid, -5 °C, and linolenic acid, -11 °C, then what structural aspect of these 18-carbon fatty acids is associated with their melting points? And also, what would be the molecular explanation for the trend in the melting points? So I'm thinking that the reason for this is because of their double bonds. How many double bonds they have would raise their melting points! This is my first post, I wonder if I got this right

Here's a question. Name 2 type of molecules that are found within the cell membrane ?

Hello again, I have extracted RNA and i am satisfied with the purity (260/280 ratio) and the yield. What is troubling me is the very low 260/230 ratio? Therefore, i wanted to know how important is the 260/230 ratio in RNA extraction. I will use the RNA for qPCR. Yield 26-35 ng/ul and 260/280 ratio is between 1.9-2.2 If i want to re-precipitate the RNA should i use sodium acetate or Lithium chloride to do this? Thanks

Hey, Having an issue with 293T cells and FGF stiumaltion assays. I grow 293T cells, transfect them with expression vectors containing proteins that i know increase response to FGF stimulation compared to untransfected cells, and i know that 293T cells endogenously express fgf receptor. i know the expression vectors express protein, via western blot, but the response to FGF stimulation after incubation in minimal media (krebs HEPES buffer) does not follow the expected outcome. when comparing even untransfected, non-fgf treated cells with fgf treated cells, no increase in pERK is seen (considered the measurable output for fgf stimulation), so the issue seems to be with…

hello guys! I need to prepare a 2 mg/mL DNAase solution. the DNAase stock has a concentration of 2U/ml. how can i make a 2mg/ml from a 2U/ml solution? this is quite hard to figure out hope very much for replies! thanks for helping!

Dear Colleagues, We are proud to announce the 2nd Horizons-Meeting entitled "Decoding Nature: Hierarchy of Interactions". The meeting will be held at the Max-Planck Institute for Biophysical Chemistry in Göttingen, Germany on 17th - 19th of March 2005. Our meeting aims to provide deep insights into contemporary life science research and to encourage exchange among people from different biological science branches. We invite distinguished scientists from fields as diverse as structural biology, developmental biology, cell biology and neurobiology. By including such a diverse spectrum of topics we hope to broaden horizons and to inspire interdisciplinary discussions. T…

Detailed answers would be appreciated: 1) What is the difference, in terms of data obtained, between Coomassie Blue staining and Western blotting. 2) What kind of important information can be obtained from purifying a protein from the native species itself? 3) Why is it not a good idea to use too much of template DNA for a PCR reaction? Any help would be appreciated, thank you

Hi all! I have a new protein sequence and I would like to know if someone have tried or know some program that could predict theoritically the 3D structure from the aminoacid sequence. I found some stuff at google.. but those are freaking complicated... Thanks

Hi, I was lately trying to do a rescue of a gene knock out I did in yeast. I got a good phenotype from the knockout but could not restore the knockout by cloning the gene back in. Now I am trying to figure out why. Originally I cloned my gene into a yeast vector which uses a uracil marker, but was instructed to remove the uracil marker from my knock out first since it already had that one. So I cultured the yeast in medium with 5'-floroorotic acid which selects against ura3 producing cells. This was supposed to remove the marker, so I cultured the colonies in rich media and then did a transformation using the LiAc method. At that point I believed I had my knock out wi…

Quick fire question, what does each do....5-HT/1....5-HT/2-a/b/c et cetera Theres triptans that block migraines that work on the 5-HT/1's but purpose built to not act on 2-a/b/c?'s is it that 1's work in different particular neurological pathways to the 2's or such?? My biochem and neurology are in infancy so analogies work well Regards. UPDATE: Do the selective 5-HT,2/b agonist's give a non-psychedelic feeling? And what does blood brain barrier mean?

anybody have an image of the 5HT1 receptor? i need to know the structure of it ASAP. if you find it, i would be most grateful. thank you.

I need to make a series of 6M and 8M guanidinium HCl buffers for a protein purification, but I can't seem to get the GdnHCl into solution. I'm able to make a clear 8M solution, but when I make the 6M solution, theres some sort of cloudy precipitate that eventually settles. I've tried using the 8M solution as a stock and diluting it down to 6M, but I've noticed that it starts to crash out at around 7.3M. Heating it to 35C doesn't make a difference, nor does stirring it for several days. Is there some trick to it or has my GdnHCL probably gone bad?

Dear scientists, I need a Complete List of the Molecules that build SARS-CoV2 (not only its spikes', but ALL of its molecules). I am not sure if that list is called molecular structure. The molecular structure of HIV or HSV or any other human viruses are also desirable, but my first priority is SARS-CoV 2. I would really appreciate if someone can help me find those lists. Your answers may save lives! Best regards and stay safe

Okay so I have a school assignment on the morality of the whole genetic engineering scene, Its the good old debate, is it moral? Who out there is willing to give us some opinions, I do ask though that you post whether or not your happy to be quoted on your opinions, Best regards The Corporal