Jump to content

pfluffernuugen

Members
  • Posts

    2
  • Joined

  • Last visited

Retained

  • Lepton

pfluffernuugen's Achievements

Lepton

Lepton (1/13)

10

Reputation

  1. The water should be fine. It's from a milliQ system with a measured resistance of around 18MOhm
  2. I need to make a series of 6M and 8M guanidinium HCl buffers for a protein purification, but I can't seem to get the GdnHCl into solution. I'm able to make a clear 8M solution, but when I make the 6M solution, theres some sort of cloudy precipitate that eventually settles. I've tried using the 8M solution as a stock and diluting it down to 6M, but I've noticed that it starts to crash out at around 7.3M. Heating it to 35C doesn't make a difference, nor does stirring it for several days. Is there some trick to it or has my GdnHCL probably gone bad?
×
×
  • Create New...

Important Information

We have placed cookies on your device to help make this website better. You can adjust your cookie settings, otherwise we'll assume you're okay to continue.