Silvia_84 Posted March 30, 2016 Share Posted March 30, 2016 Hi all, I extracted DNA and now I have a stock solution in TE buffer and two aliquots in water. How is it better to store all these solutions since I need to use it for long periods of time? Freezer or fridge? Thank you Link to comment Share on other sites More sharing options...
foxy john Posted March 30, 2016 Share Posted March 30, 2016 Put it in the freezer. Link to comment Share on other sites More sharing options...
Arete Posted March 30, 2016 Share Posted March 30, 2016 Because freeze-thaw cycles can shear DNA, if you're using it a lot, keep it in the fridge. If it's medium term storage, -20, and I keep my long term stored samples in the -80. Link to comment Share on other sites More sharing options...
CharonY Posted March 30, 2016 Share Posted March 30, 2016 Actually I think that for a number of reasons the shearing due to freeze-thaw itself is only a secondary concern, but rather minute impurities tend to do the damage during the warm-up phase. In practical terms, there is not difference, of course. For extremely long storage (years to decade) I found DNA to be quite stable (at least whole bacterial chromosomes as well as plasmids) as precipitated under ethanol at -80C or carefully freeze dried. I presume that this is not the scope being asked, though. Link to comment Share on other sites More sharing options...
BabcockHall Posted March 31, 2016 Share Posted March 31, 2016 As a general rule when I freeze a protein, I try to freeze it quickly. I would imagine that some of the same considerations apply to freezing DNA. Link to comment Share on other sites More sharing options...
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