Gamewizard Posted December 6, 2011 Share Posted December 6, 2011 -------------------------------------------------------------------------------- Hi, Can someone please tell me if I am doing the right calculation here. So, the question is if had to make 50ml of a 2.0 % agarose gel, how much agarose would I need to weigh out? So I find 2% of 50ml:- 2.0x50ml = 100 g of agarose ? is this correct ? And I need to calculate how much of a 6 X loading buffer I need to add to my PCR sample, but I am not sure how to do that. Can someone please help? Many thanks Link to comment Share on other sites More sharing options...
CharonY Posted December 6, 2011 Share Posted December 6, 2011 For a 2% gel you need to know the weight of 50 ml water and take 2% of that weight in agarose. There are different types of loading buffers (though with overall similar to same composition). As a rule of thumb X-buffers are used in 1x final concentration (though in cases of some universal buffers, they are not). Link to comment Share on other sites More sharing options...
Greippi Posted December 8, 2011 Share Posted December 8, 2011 When making gels, I assume 1ml of water = 1g. So your answer would be correct. Link to comment Share on other sites More sharing options...
jimmydasaint Posted December 8, 2011 Share Posted December 8, 2011 Hang on, I thought the % method referred to weight per volume, e.g. 5% of agarose would represent 5 g dissolved in, and made up to 100 ml. I don't think I need to say more because this is homework help. Link to comment Share on other sites More sharing options...
CharonY Posted December 8, 2011 Share Posted December 8, 2011 Yes, Jimmy is right. Link to comment Share on other sites More sharing options...
Recommended Posts
Create an account or sign in to comment
You need to be a member in order to leave a comment
Create an account
Sign up for a new account in our community. It's easy!
Register a new accountSign in
Already have an account? Sign in here.
Sign In Now