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How to improve DNA gel extraction process?


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Hey guys! ūüĎ謆

Does anybody know any tip to increase DNA yield from agarose gel? I use Promega Kit and short fragments clean up properly but when it comes to linear plasmid DNA (>5000 bp), yields are poor.

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1 hour ago, Tim S said:

Thank you for the answer! Looks very comprehensive. But the point is that, it doesn't work as the describe it unfortunately 

"Looks comprehensive" is an admission that you didn't read it, which seems odd if you really want an answer. Should we assume you haven't contacted Promega about this problem with their product?

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I actually have read it very carefully. Thank you! By saying "very comprehensive" I meant I have never seen this long and meticulous article through I have read a lot of Promega protocols on this topic. 

Answering to your question- no, I have not contacted to Promega. I have read the text. And I mean their protocol specifics like "Persent Recovery" do not match to my experience of working with their Kits

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37 minutes ago, Tim S said:

I actually have read it very carefully. Thank you! By saying "very comprehensive" I meant I have never seen this long and meticulous article through I have read a lot of Promega protocols on this topic. 

You're welcome! Perhaps we have different definitions of "very comprehensive", but I wouldn't describe an article that way if it didn't address my concerns over poor yields when using their product.

41 minutes ago, Tim S said:

Answering to your question- no, I have not contacted to Promega. I have read the text. And I mean their protocol specifics like "Persent Recovery" do not match to my experience of working with their Kits

Are you looking to add other voices to your complaint about their product before contacting them directly?

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  • 2 months later...

I believe the silica spin columns for gel extraction are optimised for a certain DNA fragment size range. I can't remember whether the gel extraction kit columns are better for small or large fragments, but your could try using miniprep spin columns instead because these will be optimised for larger fragments.

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  • 1 month later...

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